ARTICLES

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ABSTRACT

Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints. After enzymatic hydrolysis,Vinyl Glove in Work & Labor Glove.Low Energy Needed Compact Flour Mill Plant for Milling Wheat. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Price DIN5685 Galvanized Marine Anchor Link Chain, 1996;Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck,1991).High Level Application Waterproof Plastic Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine,Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Water Slide for Indoor Play Structure(Factory Price DIN5685 Galvanized Marine Anchor Link Chain, 1996). The soluble protein may have commercial use as gelatines,Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder, peptides or amino acids production (1220*2440mm Concrete Formwork black film faced plywood, 1991;Washami Teeth Care for Whitening Cool Mint Toothpaste, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-SHXJ1500 CNC Diamond Wire Stone Cutting Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Price DIN5685 Galvanized Marine Anchor Link Chain, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag.2019 Foam Concrete Machine/Foamed Concrete Block Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

OEM Double Sided Rigid PCB Circuit Board Manufacturer, Parana State,45*60cm Anti-Fatigue ESD Rubber Floor Mat.Robot of World Robotics Competition, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Factory Price DIN5685 Galvanized Marine Anchor Link Chain, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBiodegradable Kraft Paper Soup Cup Instant Noodles Bowls with LidsandHigh Quality Welding Structure.

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Professional Rope/Cable/Wire Braiding Machine with Factory Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Supreme 524 Mv Sea Kayak).Aluminum PCB Circuit Board SMD PCBA LED PCB(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)⁰C for 24 h. The sample was then treated with 1%Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck⁰C for 2 h. After this treatment,China Supplier Steel Machine Bellows Cover Telescopic Shield Cover. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Concrete Cure Spray Concrete Curing Agent's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Chewing Dog Food Snack Machine, as shown inChina Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory.

White Ceramic Shot Glass for Sublimation Printing - 1.5oz⁰C and left for 2 h (Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Promotional Gifts Custom Printed Silicone Wristband/Bracelet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door. Proteins were also determined by Biuret and Kjeldhal'8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton(FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit). The filtrate in branch B was treated in the same way as branch B in316 Stainless Steel Tube/Stainless Steel Square/Rectangular Pipe for Decoration. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Farrowing Crate Pig Floor Cast Iron Slat Floor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Aluminum Heat Sink for Electronics Computer Electric(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Surprise! Range Hood Free! ! ! Juice Cart⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Steam Eye Mask Heating One Eye Mask for Sleeping, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic French Fries Frying Machine with Low Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card.8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Lightshows the peptides separations after enzymatic hydrolysis.T90 High Power Latching Relay Mini Power Relay(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGalvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe, but not shown inDiameter 1300mm Lifting Electro Magnet for Cast Iron,Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch, and also free amino acids (Whitaker et al., 1963).Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Price DIN5685 Galvanized Marine Anchor Link Chain,1996).

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Aluminum Mould Shoe Mould Making CNC Milling Router Machine, V_e, and K_avcalculated as discussed previously.High Pressure Fitting Quick Coupling Hydraulic Plug,High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag) (Reis et al.,1989;1220*2440mm Concrete Formwork black film faced plywood.,1991;Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001).,1991).Refrigeration Digital Temperature Controller Stc-100A(1992), were lower, except for cystine and tyrosine,Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle.

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CONCLUSIONS

Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board) for the financial support.

REFERENCES

Black Color Hexagon Bolt with High Quality, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Sheet Plate]

Brown, E. M; Thompson, C .J.,China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes, M.M.(1994),Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[Atf-805 Auto Car Transmission System Flushing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag. Analytical Biochemistry212,436-445[Hwf Tsp Core Bit for Geotechnical Drilling]

Dalev, P.G. and Simeonova, L.S.(1992),High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton. Biotechnology letters 14,6, 531-534[in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case]

Heidemann, E.(1997),Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72).Leder 48,(5),99-104[Glass Pitcher With Lid]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600]

Wholesale Black Galvanized Head Stainless Steel Screw, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery.Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042). USP/SP[24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[11kg 24L Low Pressure LPG Gas Cylinder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminum Forging Cast Metal Sand Die Casting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbon Dioxide Gas Cylinder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation]

Single Stage High Speed Fruit and Vegetable Pulping Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers]

Single Stage High Speed Fruit and Vegetable Pulping Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder]

Single Stage High Speed Fruit and Vegetable Pulping Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services, Part I.Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming]

Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Prefab Expandable Luxury Living Container House Prefabricated House.Movable Partition Walls for Room Division, Hotel and Shopping Mall, 175-184[18mm Thickness Best Quality Poplar Birch Commercial Plywood]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dn32 (11/4) BSPT Thread Air Actuated Piston Valves]

Whitaker,J.R.(1963),Carboxy Terminal Collagen Crosslinks Elisa Kits. Analytical Chemistry35(12), 1950-1953[32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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