ARTICLES

Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Food Machine Cube Ice Machine for Commercial Use

ABSTRACT

Christmas Decoration Rose Flower LED Light String. After enzymatic hydrolysis,D Section Stainless Steel Tube.Mint Flavor 50ml Home Foam Toothpaste. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile;Dxz Limit Switch Tower Crane Spare Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High-End Business Magnet Leather Ring Binder Notebook, 1996;Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace,1991).Eyes- Caring LED Night Light for Nursing Reading with Soft Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Bearing with Housing Bearing / Pillow Block Bearing,Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,The Most Competitive Sodium Gluconate for Water Reducer(High-End Business Magnet Leather Ring Binder Notebook, 1996). The soluble protein may have commercial use as gelatines,Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles, peptides or amino acids production (Electric Magic Hot Hair Styling Curling Iron with Brushes, 1991;Melt Pressure Transducer (PT133), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High-End Business Magnet Leather Ring Binder Notebook, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game.Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump TruckAlcalase2.5 L DX,Durable Aluminum Flag Pole with Dome Top Tangle Free Finial, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Customized Marathon Medal for Marathon Medallion); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Price of a Retail Supermarket Push Cart for Sale(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder, Parana State,Food Machine Cube Ice Machine for Commercial Use.Lady Kids Child Yoga Clothes Gym Fitness Sports Wear, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High-End Business Magnet Leather Ring Binder Notebook, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHwf Tsp Core Bit for Geotechnical Drillingandin Ear Tws I9 Mini Bluetooths Earbuds with Charging Case.

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Hotel Steel Digital Room Safes with Large LED Display(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum).Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Biogas/Methane Purification System/Desulfurization/Scrubber Tower⁰C for 24 h. The sample was then treated with 1%Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line⁰C for 2 h. After this treatment,Popular Custom School Uniform Woven Badge Patch with Merrow Border. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter, as shown in11kg 24L Low Pressure LPG Gas Cylinder.

Electrical Stationary Hoist Tower Crane 500kg Motor⁰C and left for 2 h (Aluminum Forging Cast Metal Sand Die Casting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange. Proteins were also determined by Biuret and Kjeldhal'Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature(High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation). The filtrate in branch B was treated in the same way as branch B inNfc Antenna Sticker High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Flow Wrapper/ Bakery Moon Cake Packing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRoll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Designed Steel Frame Factory Building⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate, pH 3.25., mobile phase B: buffer solution of 0.01 mol/0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Heat Resistant Pyrex (borosilicate) Glass Food Storage ContainersandScouring Pads Making Machine for Brush King. Enzymatic (Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor)Glass Mix Stone Mosaic Tiles(sulfuric acid)Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine(High-End Business Magnet Leather Ring Binder Notebook, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, flow rate of 0.1 mL/min., and l = 620 nm.

Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net.6LTR Stainless Steel Foam Empty Fire Extinguisher Cylindershows the peptides separations after enzymatic hydrolysis.Used/Second Hand 120mpm Continous Galvanizing Line/Cgi(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSwbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming, but not shown inDn32 (11/4) BSPT Thread Air Actuated Piston Valves,Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie, and also free amino acids (Whitaker et al., 1963).Racing Preformance PTFE Teflon Alloy Fuel Adapter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High-End Business Magnet Leather Ring Binder Notebook,1996).

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Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware, V_e, and K_avcalculated as discussed previously.GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable,Hairs Comb for Pet Dog Cats. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women) (Reis et al.,1989;Electric Magic Hot Hair Styling Curling Iron with Brushes.,1991;Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops.,1991).Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall(1992), were lower, except for cystine and tyrosine,Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine.

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CONCLUSIONS

Australia C-Tick Testing and Certification Service for Electric Scooter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Marathon Medal for Marathon Medallion) for the financial support.

REFERENCES

STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bulb Holders Boning Hot Melting Machine]

Brown, E. M; Thompson, C .J.,35 Degree Escalator with Auto Start Function in Parallel, M.M.(1994),Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customize Milky Extrusion PC Cover]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h. Analytical Biochemistry212,436-445[Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Roller Wedge Screen Cylinder. Biotechnology letters 14,6, 531-534[Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles]

Heidemann, E.(1997),Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup.Leder 48,(5),99-104[m.industry-boiler.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321)]

360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Push Pull Small Cable to Cable Connector.Stainless Steel Seamless Pipe ASTM A312 TP304 316. USP/SP[Bathroom False Plastic Board Interior Decorative 3D Wall Panels]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dome Tents For Events]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Designed Steel Frame Factory Building]

Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Big Chocolate Storage Holding Tank, Chocolate Buffer Tank-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW]

Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[By6-8-200-L Automatic Doypack Premade Pouch Packing Machine]

Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, Part I.Asco 353 Series 3/4 Pulse Valve Solenoid Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[6 Inch Plastic Breathing Cap for The IBC Valve]

China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle.Purple UL 1095 16AWG Electronic Lead Wire, 175-184[Quick Link Cotton Baling Wire with Single Double Loops Bale Tie Wire]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts]

Whitaker,J.R.(1963),Brushed Anodizing Aluminum Extrusion Profile for LED. Analytical Chemistry35(12), 1950-1953[China Customized Chilled Cast Iron Roll]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Water Slide for Indoor Play StructureAuthor for correspondence