ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10

ABSTRACT

360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1). After enzymatic hydrolysis,Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW).Food Machine Cube Ice Machine for Commercial Use. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Christmas Decoration Rose Flower LED Light String;D Section Stainless Steel Tube; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mint Flavor 50ml Home Foam Toothpaste, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile, 1996;Dxz Limit Switch Tower Crane Spare Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock,1991).High-End Business Magnet Leather Ring Binder Notebook. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops,New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Eyes- Caring LED Night Light for Nursing Reading with Soft Light(125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile, 1996). The soluble protein may have commercial use as gelatines,China Bearing with Housing Bearing / Pillow Block Bearing, peptides or amino acids production (Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800, 1991;The Most Competitive Sodium Gluconate for Water Reducer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Electric Magic Hot Hair Styling Curling Iron with Brushes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Melt Pressure Transducer (PT133). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe.Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Durable Aluminum Flag Pole with Dome Top Tangle Free Finial, Parana State,Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10.Customized Marathon Medal for Marathon Medallion, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theVenlo Type Flower Multi Span Commercial Glass Greenhouses UsedandWhat′s Kind of Machine for Making Organic Fertilizer Granules.

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Price of a Retail Supermarket Push Cart for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Evaporator Finned Tube).Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Lady Kids Child Yoga Clothes Gym Fitness Sports Wear⁰C for 24 h. The sample was then treated with 1%Hotel Steel Digital Room Safes with Large LED Display⁰C for 2 h. After this treatment,Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Biogas/Methane Purification System/Desulfurization/Scrubber Tower's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line, as shown inMagnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive.

Popular Custom School Uniform Woven Badge Patch with Merrow Border⁰C and left for 2 h (60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter. Proteins were also determined by Biuret and Kjeldhal'Electrical Stationary Hoist Tower Crane 500kg Motor(Cog Graphic FPC LCD Display Module). The filtrate in branch B was treated in the same way as branch B inm.agapassworld.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Reusable Metal Stainless Steel Drinking Straws, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219bandDesigned Steel Frame Factory Building. Enzymatic (Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate)0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table(sulfuric acid)Scouring Pads Making Machine for Brush King(125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor, flow rate of 0.1 mL/min., and l = 620 nm.

Glass Mix Stone Mosaic Tiles.K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Maskshows the peptides separations after enzymatic hydrolysis.Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc, but not shown inEyes- Caring LED Night Light for Nursing Reading with Soft Light,3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, and also free amino acids (Whitaker et al., 1963).Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile,1996).

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YAMAHA Yv100II Nozzle Horder Square Head Short TypeandGlazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panelshow the gel chromatographic separation of soluble protein with Sephadex G-Used/Second Hand 120mpm Continous Galvanizing Line/Cgi.Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie, and another peak at 45 mL not shown inCorrugated Board Production Line, for peptides lower than 3000 Da.Racing Preformance PTFE Teflon Alloy Fuel Adapter(Condell et al.,1993) .

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Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170, V_e, and K_avcalculated as discussed previously.High Quality Stainless Steel Expanded Metal Mesh,Popular Lovely Animal Design Inflatable Slide for Kids. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe) (Reis et al.,1989;Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800.,1991;Dxz Limit Switch Tower Crane Spare Parts.,1991).Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware(1992), were lower, except for cystine and tyrosine,GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable.

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CONCLUSIONS

Hairs Comb for Pet Dog Cats. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord) for the financial support.

REFERENCES

Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Full Spectrum CBD Oil]

Brown, E. M; Thompson, C .J.,Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine, M.M.(1994),Australia C-Tick Testing and Certification Service for Electric Scooter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,. Analytical Biochemistry212,436-445[Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery]

Dalev, P.G. and Simeonova, L.S.(1992),35 Degree Escalator with Auto Start Function in Parallel. Biotechnology letters 14,6, 531-534[Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant]

Heidemann, E.(1997),Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings.Leder 48,(5),99-104[m.daftonsmt.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull]

Good Price Stand-up Zipper Doy Pouch Filling Packing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h.Roller Wedge Screen Cylinder. USP/SP[ADC12 Aluminum Die Casting Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Safest Baby Healthcare and Grooming Kit for Baby Gift]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Plastic PVC Fiber Water Hose Pipe Tube Packing Wrapping Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Original Testo 174t Mini Temperature Data Logger No. 0572 1560]

Push Pull Small Cable to Cable Connector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Seamless Pipe ASTM A312 TP304 316-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Vitamin D3 Fat-Solubility Water-Solubility Feed Additive]

Push Pull Small Cable to Cable Connector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card]

Push Pull Small Cable to Cable Connector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco, Part I.Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric]

Big Chocolate Storage Holding Tank, Chocolate Buffer Tank, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce.Asco 353 Series 3/4 Pulse Valve Solenoid Valve, 175-184[Adding Length with Nut Coupler]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine]

Whitaker,J.R.(1963),China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material. Analytical Chemistry35(12), 1950-1953[Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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