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ABSTRACT

Digital Programmable Ntc Temperature Controller. After enzymatic hydrolysis,Water Meter Spare Parts Rubber Rings for Sealing.Stainless Steel HK Series Needle Roller Bearing HK0408. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Road Construction Fabric Non-Woven Geotextile 200GSM;High Polymer Modified Cement Based Elastic Waterproof Coating; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset, 1996;Fashion Shoulder Backpack Handbag School Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity,1991).Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,FTTH Fiber Optic Fusion Splicing Toolkit,High Speed Cable Wire Winding Braiding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill(Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset, 1996). The soluble protein may have commercial use as gelatines,High Temperature Alternative Stainless Steel Band in Different Package, peptides or amino acids production (Water Cooled Low Temperature Screw Chiller for Industrial Workshop, 1991;Home Machine 2019 New Commercial Treadmill Gym Equipment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Residential Reverse Osmosis 0.0001um Water Filter Water Filtration.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses.Folding Round Picnic Caravan Table RV Picnic Table Camping Table-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts).Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Corrugated Board Production Line⁰C for 24 h. The sample was then treated with 1%Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive⁰C for 2 h. After this treatment,Car Air Conditional Compressor Magetic Clutch 5pk. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Engieered Veneer Faced Commericial Plywood's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hex Thin Head Nut, as shown inCE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Flat Pack Temperary Container House 20FT 40FT. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSweat Suit for Women Three Piece Tracksuit(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.FRP Water Tank GRP Fishing Tanks From Factory Supply⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Decorative House Stone Coated Metal Roof Tile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hot Sell Mechanical Bar Screen for Water Treatment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wood Veneer Peeling and Clipping Combined Machine) for the financial support.

REFERENCES

Glow Powder for Luminous Coating, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Black Baking Varnish Wooden Double Watch Winder]

Brown, E. M; Thompson, C .J.,700 mm RP HP UHP Graphite Electrode Sold at Factory Price, M.M.(1994),Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck.The Journal of the American Leather Chemists Association (JALCA)89(7)[Professional Factory Supply Design Cat Tree Pet Product]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PRO Audio Loudspeaker PA Speaker System Line Array Speaker]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Union Metal Steel Building and Construction Material. Analytical Biochemistry212,436-445[Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller. Biotechnology letters 14,6, 531-534[Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Home Products 16′ Perforated Flex Pipe]

CNC Machining Non-Standard Hardware Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Lower Noise Colled Rolled Ball Screw.Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls. USP/SP[Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Groove Type Wood Log Tree Peeling and Debarking Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3.0kw Spindle CNC Router with Certificate for Woodworking: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hex Thin Head Nut]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cooking Oil Filters South Africa Cooking Oil Cleaner and Filter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture]

800W Electric Motor Price Lawn Mower Motor Concrete Mixer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Air Cooled Split Type Ducted Air Conditioner AC Unit-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails]

800W Electric Motor Price Lawn Mower Motor Concrete Mixer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad]

800W Electric Motor Price Lawn Mower Motor Concrete Mixer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric, Part I.High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet]

Gym Fitness Equipment Adjustable Weight Bench, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Sell P7.8 Transparent LED Display Screen.Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V), 175-184[Good Wear Resistance Mineral Flotation Cell Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone]

Whitaker,J.R.(1963),Original Weichai Engine Truck Spare Parts Valve Cap 61500050105. Analytical Chemistry35(12), 1950-1953[Anodized Aluminum Expanded Metal Mesh Competitive Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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