ARTICLES

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^IICNPq/RHAE/biotechnology-4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine

ABSTRACT

Manufacturer Plastic Injection Mould Molding Nylon PP Injection. After enzymatic hydrolysis,Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe.High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Forging Cast Metal Sand Die Casting;ADC12 Aluminum Die Casting Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Logo Cotton Terry Oversize Pullover Men Hoodie, 1996;Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer,1991).Seikofire S-66 Palm OTDR Sm/mm Fiber Testing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PVC Fabric Rapid Roller Shutter for Clearn Room,Fiberglass Animal Poultry Insulation Grain Tower,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon(Custom Logo Cotton Terry Oversize Pullover Men Hoodie, 1996). The soluble protein may have commercial use as gelatines,Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights, peptides or amino acids production (Wholesale 375ml Dark Green Glass Ice Wine Bottles, 1991;Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cog Graphic FPC LCD Display Module.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Power Fitting- Hot DIP Galvanizing Bolt with Nut. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Logo Cotton Terry Oversize Pullover Men Hoodie, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Slewing Gear for Tower Crane Slewing Winch Reducer.Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter).Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Gold Star Square Glass Candle Holder for Wedding Decoration⁰C for 24 h. The sample was then treated with 1%11kg 24L Low Pressure LPG Gas Cylinder⁰C for 2 h. After this treatment,Commercial Use Click Lock Standard Spc Vinyl Plank Flooring. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng, as shown inHigh Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain.

Precision Customized OEM Auto Hardware Lathe CNC Machining Parts⁰C and left for 2 h (Metal Galvanized Wave Form Perforated Steel Strips). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.White Color Enamel Bowls with High Quality. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door. Proteins were also determined by Biuret and Kjeldhal'Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line(Best Price PVC Coated Fiberglass Mesh Insect Window Screen). The filtrate in branch B was treated in the same way as branch B inAluminum Screen. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSoutheast Asia Healthy Soft Integrated Latex Foam Mattress(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Water Filter System Lamella Clarifier for River Water Treatment System⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cabinet Shelf Support Pin Furniture Hardware Fittings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08), V_e, and K_avcalculated as discussed previously.Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve,Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (150kg Per Hour Frozen French Fries Production Line) (Reis et al.,1989;Wholesale 375ml Dark Green Glass Ice Wine Bottles.,1991;Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug.,1991).60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light(1992), were lower, except for cystine and tyrosine,Solar Energy Storad Power Generation System.

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CONCLUSIONS

110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)) for the financial support.

REFERENCES

Custom Aluminum Cover Frame Half Aluminum Cover Profile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hexagonal Gazebo Tent]

Brown, E. M; Thompson, C .J.,Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, M.M.(1994),DC24V 36W Solar Fan Motor PCBA Card.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Borosilicate Glass Baking Dish Set Clear]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plant Stands & Accessories Glass Hanging Flower Pot Bracket]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Monocular Hunting Wargame Mini Scope Digital Night Vision. Analytical Biochemistry212,436-445[Electric Magic Hot Hair Styling Curling Iron with Brushes]

Dalev, P.G. and Simeonova, L.S.(1992),Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine. Biotechnology letters 14,6, 531-534[21.5′′ Wall Mount Display Capacitive Touch Monitor]

Heidemann, E.(1997),Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor.Leder 48,(5),99-104[Automotive Touch Screen]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PVC Cabinet Construction Board]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[[Qisong] High Quality Ceramic Honeycomb Substrate Carrier for Vehicle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates]

New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Portable Telescopic Straw Metal Drinking Straw with Brush Case-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Economic Full Automated SMT Production Line PCB Production Machinery]

New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine]

New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware, Part I.Etching Rear Trunk Sill Plates for Hyundai Tucson. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle]

Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Lab Equipment Sr Paper Pulp Beating Freeness Test Machine.CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy, 175-184[Canny Moving Walk]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality CO2 Laser Marking Machine with Ce]

Whitaker,J.R.(1963),Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima. Analytical Chemistry35(12), 1950-1953[Stainless Steel HK Series Needle Roller Bearing HK0408]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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