ARTICLES

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ABSTRACT

Factory Outlet Automatic Grain Tower Dryer Drying Equipment. After enzymatic hydrolysis,Mesh Bag Packing 20kg Pure White Garlic.Supreme 524 Mv Sea Kayak. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Diesel Generator Parts AVR Ki-Davr-95s for Kipor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Gld Brand Padlock Brass Popular in 5 Continents  , 1996;No Lead Bronze Flange Manufacturer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube,1991).Snsc Fd30 Side Shift New Diesel Forklift 3ton. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117),Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Laptop Bag College Men Backpack Leather Back Pack Bags(Gld Brand Padlock Brass Popular in 5 Continents  , 1996). The soluble protein may have commercial use as gelatines,Pet Dog Grooming Rake Dematting Tool Shedding Comb, peptides or amino acids production (Custom Apparel Applique Round Patch School Club Embroidery Collar Patch, 1991;Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Food Metal Detector Machine for Production Line. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Gld Brand Padlock Brass Popular in 5 Continents  , 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Multi-Functional Backpack Manual 52cc Brush Cutter.Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Aluminum Sheet Shearing Machine, Parana State,Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line.Electrical PV DC Solar Cable, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Gld Brand Padlock Brass Popular in 5 Continents  , 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePVC Cabinet Construction BoardandHorizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates.

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Tomato Bubble and High Pressure Washing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine).Upholstery Ribbon Christmas Tape(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate⁰C for 24 h. The sample was then treated with 1%2+1 Flavors Soft Ice Cream Machine with Pre-Cooling⁰C for 2 h. After this treatment,Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best Sale Low Price Household Air freshener Spray, as shown inRg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle.

Factory Supplied High Strength Marine Anchor Chain⁰C and left for 2 h (High Quality CO2 Laser Marking Machine with Ce). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Humidity Refrigeration Digital Controller Jsd-100+. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Elegant Europe Dessign Pillow Top Mattress. Proteins were also determined by Biuret and Kjeldhal'8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8)(Stainless Steel HK Series Needle Roller Bearing HK0408). The filtrate in branch B was treated in the same way as branch B in24V 100ah BMS Protect Circuit Module for LiFePO4 Battery. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Modern 4 Person Workstation with Computer Desk Modular Office Furniture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Frequency Steel Straight Seam Pipe Welding Line/Tube Mill(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3D CNC Router for Wood Cutting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Screens / Wedge Wire Filter Element, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Small Cleaning Brush ESD Anti Static Brush ESD Brush Black) for the financial support.

REFERENCES

Low Carbon Heavy Galvanized Steel Wire, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Selling Rice Husk Wood Charcoal Carbonization Stove with Factory Price]

Brown, E. M; Thompson, C .J.,Long Distance Measurement Power Laser Digital Meter, M.M.(1994),Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets. Analytical Biochemistry212,436-445[Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors]

Dalev, P.G. and Simeonova, L.S.(1992),LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310. Biotechnology letters 14,6, 531-534[Scouring Pads Making Machine for Brush King]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ladle Refining Melting Steel Furnace Casting Ladle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Counter-Clip Fluorine-Lined Butterfly Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Design Inclined Plane 18W LED Wall Washer Lighting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fiber Laser Cutting Machine of Metal Sheet &Pipe for Metal Processing-3000W]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard]

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2ton R Type Electric Wire Rope Hoist with Single Speed,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator]

2ton R Type Electric Wire Rope Hoist with Single Speed, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier, Part I.Adhesive CAS 9003-35-4 Phenolic Resin. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover]

Whitaker,J.R.(1963),Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys. Analytical Chemistry35(12), 1950-1953[Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

White/Black/Green Color 600GSM Polyester/PP Non Woven GeotextileAuthor for correspondence