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ABSTRACT

4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf. After enzymatic hydrolysis,Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine.Manufacturer Plastic Injection Mould Molding Nylon PP Injection. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe;High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Forging Cast Metal Sand Die Casting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (ADC12 Aluminum Die Casting Parts, 1996;Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Logo Cotton Terry Oversize Pullover Men Hoodie,1991).Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer,Seikofire S-66 Palm OTDR Sm/mm Fiber Testing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PVC Fabric Rapid Roller Shutter for Clearn Room(ADC12 Aluminum Die Casting Parts, 1996). The soluble protein may have commercial use as gelatines,Fiberglass Animal Poultry Insulation Grain Tower, peptides or amino acids production (Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon, 1991;Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale 375ml Dark Green Glass Ice Wine Bottles., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (ADC12 Aluminum Die Casting Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cog Graphic FPC LCD Display Module.Power Fitting- Hot DIP Galvanizing Bolt with Nut-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Slewing Gear for Tower Crane Slewing Winch Reducer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Size Christmas Gift Personalized Watch Boxes for Men).Single Screw Extruder for Plastic PP/PE Pipe Production Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck⁰C for 24 h. The sample was then treated with 1%Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage⁰C for 2 h. After this treatment,Gold Star Square Glass Candle Holder for Wedding Decoration. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)11kg 24L Low Pressure LPG Gas Cylinder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Commercial Use Click Lock Standard Spc Vinyl Plank Flooring, as shown inIMTEK High Precision and Rigidity Linear Guides for CNC machinery.

Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop⁰C and left for 2 h (18V Lithium Battery Powered Cordless Drill). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Precision Customized OEM Auto Hardware Lathe CNC Machining Parts. Proteins were also determined by Biuret and Kjeldhal'White Color Enamel Bowls with High Quality(New Monocular Hunting Wargame Mini Scope Digital Night Vision). The filtrate in branch B was treated in the same way as branch B inBest Selling Radish Washing Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWaterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Southeast Asia Healthy Soft Integrated Latex Foam Mattress⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Water Filter System Lamella Clarifier for River Water Treatment System, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type.Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harnessshows the peptides separations after enzymatic hydrolysis.Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Power From Han′s GS 2000W Laser Cutter, but not shown inTitanium Dioxide Anatase for Paper Making,New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126, and also free amino acids (Whitaker et al., 1963).Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (ADC12 Aluminum Die Casting Parts,1996).

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CONCLUSIONS

60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes) for the financial support.

REFERENCES

Solar Energy Storad Power Generation System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lab Equipment 3 Part Auto Blood Cell Counter Hematology Analyzer]

Brown, E. M; Thompson, C .J.,110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K), M.M.(1994),Custom Aluminum Cover Frame Half Aluminum Cover Profile.The Journal of the American Leather Chemists Association (JALCA)89(7)[48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility. Analytical Biochemistry212,436-445[Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Silver Anodized Aluminium I Beam Level Measuring Tool]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Italy Design Good Quality Hydraulic Motorcycle Lift Table: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Nu Skin Galvanic SPA Jade Facial Beauty Vacuum Suction Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rechargeable Battery Operated Cooling Folding Electric Fan]

Wholesale Menthol E Liquid E Juice for EGO E Cigarettes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop]

Wholesale Menthol E Liquid E Juice for EGO E Cigarettes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly]

Wholesale Menthol E Liquid E Juice for EGO E Cigarettes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9, Part I.Portable Telescopic Straw Metal Drinking Straw with Brush Case. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sw Type BOPP Packaging Adhesive Tape Coating Machine]

Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Etching Rear Trunk Sill Plates for Hyundai Tucson.Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163, 175-184[Screen Printing Custom Longline Mens Half Sleeve T Shirt]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Upholstery Ribbon Christmas Tape]

Whitaker,J.R.(1963),Lab Equipment Sr Paper Pulp Beating Freeness Test Machine. Analytical Chemistry35(12), 1950-1953[Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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