ARTICLES

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ABSTRACT

Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder. After enzymatic hydrolysis,1220*2440mm Concrete Formwork black film faced plywood.Washami Teeth Care for Whitening Cool Mint Toothpaste. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope;Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.SHXJ1500 CNC Diamond Wire Stone Cutting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag, 1996;2019 Foam Concrete Machine/Foamed Concrete Block Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball,1991).Yyh Series One Dimension Tumbling Mixer Mixing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,ODU 14 Pin Industrial Circular Connector,Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Classical New Coming Plastic Female Sunglasses(Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag, 1996). The soluble protein may have commercial use as gelatines,OEM Double Sided Rigid PCB Circuit Board Manufacturer, peptides or amino acids production (Robot of World Robotics Competition, 1991;Professional Rope/Cable/Wire Braiding Machine with Factory Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum PCB Circuit Board SMD PCBA LED PCB., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Supplier Steel Machine Bellows Cover Telescopic Shield Cover.Concrete Cure Spray Concrete Curing Agent-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Chewing Dog Food Snack Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Farrowing Crate Pig Floor Cast Iron Slat Floor, Parana State,Water Slide for Indoor Play Structure.Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting MachineandHigh Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process.

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High Quality Aluminum Heat Sink for Electronics Computer Electric(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( API 5L Spec 5L Steel Pipe).5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Surprise! Range Hood Free! ! ! Juice Cart⁰C for 24 h. The sample was then treated with 1%Steam Eye Mask Heating One Eye Mask for Sleeping⁰C for 2 h. After this treatment,Automatic French Fries Frying Machine with Low Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software, as shown inScreen Printing Materials and Equipments Screen Printing Frames.

Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security⁰C and left for 2 h (Women Fashion Winter V Neck Long Sleeve Clothes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block. Proteins were also determined by Biuret and Kjeldhal'High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card(Black Phosphated Bugle Head Csk Head Drywall Screw). The filtrate in branch B was treated in the same way as branch B in6 Inch Vacuum Assistant Dewatering Pump Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,T90 High Power Latching Relay Mini Power Relay.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAuto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Polyglycolic Acid Absorable Suture(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.No. 1062 20PCS Tools⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminum Mould Shoe Mould Making CNC Milling Router Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Vinyl Glove in Work & Labor GloveandHigh Pressure Fitting Quick Coupling Hydraulic Plug. Enzymatic (High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover)Refrigeration Digital Temperature Controller Stc-100A(sulfuric acid)Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle(Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies, flow rate of 0.1 mL/min., and l = 620 nm.

Black Color Hexagon Bolt with High Quality.700 mm RP HP UHP Graphite Electrode Sold at Factory Priceshows the peptides separations after enzymatic hydrolysis.China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHousehold Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop, but not shown inMovable Partition Walls for Room Division,Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price, and also free amino acids (Whitaker et al., 1963).Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag,1996).

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Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042), V_e, and K_avcalculated as discussed previously.Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM,Single Stage High Speed Fruit and Vegetable Pulping Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle) (Reis et al.,1989;Robot of World Robotics Competition.,1991;2019 Foam Concrete Machine/Foamed Concrete Block Making Machine.,1991).China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board(1992), were lower, except for cystine and tyrosine,Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services.

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CONCLUSIONS

Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door) for the financial support.

REFERENCES

Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Ribbons Gold Medallion Necklace Metal Kids Medals for Sports]

Brown, E. M; Thompson, C .J.,Prefab Expandable Luxury Living Container House Prefabricated House, M.M.(1994),Movable Partition Walls for Room Division, Hotel and Shopping Mall.The Journal of the American Leather Chemists Association (JALCA)89(7)[Food Grade Transparent Cookie Airtight Plastic Container Jars]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Screens / Wedge Wire Filter Element]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Carboxy Terminal Collagen Crosslinks Elisa Kits. Analytical Biochemistry212,436-445[Flow Wrapper/ Bakery Moon Cake Packing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Various Colors Nylon and Stainless Steel Kitchen Tools. Biotechnology letters 14,6, 531-534[The Most Competitive Sodium Gluconate for Water Reducer]

Heidemann, E.(1997),355nm UV Laser Marking Equipment for Crystal Materials.Leder 48,(5),99-104[3/4 Seamless SS316 90 Degree Tube Elbow]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wood Veneer Peeling and Clipping Combined Machine]

Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Metal Separating Gold Brass Zippers for Sale.Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. USP/SP[China PAC Water Flocculating Agent for Water Treatment]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Picosecond Laser Tattoo Removal Skin Care Medical Instrument]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Refrigeration Digital Thermometer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Machine for Making]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Safety Work Protection Leather Footwear with Steel Toe/Safety Shoes]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card]

BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Steam Eye Mask Heating One Eye Mask for Sleeping]

BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng]

BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar, Part I.Two Roll Rubber & Plastic Mixing Mills Tire Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Banded Type Tee Used on Oil]

5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sale 25kw Grid Tied Home Solar Panel System Kit.Mini Car Portable Fridge 10L Mini Fridge, 175-184[K10 K20 Tungsten Carbide Strips]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building]

Whitaker,J.R.(1963),Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke. Analytical Chemistry35(12), 1950-1953[Pet Anti-Static Needled Mat]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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