ARTICLES

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ABSTRACT

CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm). After enzymatic hydrolysis,Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag.Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction, 1996;DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr,1991).White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale 100% Polyester Cartoon Kids Sleepwear,Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater(ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction, 1996). The soluble protein may have commercial use as gelatines,Laparoscopy Clip Applicator for Abdominal Surgery Uses, peptides or amino acids production (High Yield Tree Stump Crusher Machine, 1991;Diaphragm Valve for Bag Filter Since 1992, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Manufacturer Plastic Injection Mould Molding Nylon PP Injection. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe.High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminum Forging Cast Metal Sand Die Casting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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PVC Fabric Rapid Roller Shutter for Clearn Room(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine).Fiberglass Animal Poultry Insulation Grain Tower(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon⁰C for 24 h. The sample was then treated with 1%Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights⁰C for 2 h. After this treatment,Wholesale 375ml Dark Green Glass Ice Wine Bottles. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine, as shown in 100p.

Cog Graphic FPC LCD Display Module⁰C and left for 2 h (Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Power Fitting- Hot DIP Galvanizing Bolt with Nut. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Slewing Gear for Tower Crane Slewing Winch Reducer. Proteins were also determined by Biuret and Kjeldhal'Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine(Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale). The filtrate in branch B was treated in the same way as branch B inSkytone 4 Channel Digital 1u Light Weight Power Amplifier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDouble Arm Motorized Multifunction Medical Equipment for Hospital Gas System(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Single Screw Extruder for Plastic PP/PE Pipe Production Line, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Cabinet Shelf Support Pin Furniture Hardware Fittings. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Logo Cotton Terry Oversize Pullover Men Hoodie) for the financial support.

REFERENCES

Reasonable Price High Quality Nylon Cable Gland, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chiller Unit 304 Stainless Steel Immersion Coil Heat Exchanger]

Brown, E. M; Thompson, C .J.,Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan, M.M.(1994),Disposable Anesthesia Breathing Circuit Tube.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type. Analytical Biochemistry212,436-445[Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse]

Dalev, P.G. and Simeonova, L.S.(1992),Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element. Biotechnology letters 14,6, 531-534[Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-DC Brushless Motor for CNC Machine with ISO9001: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Bearing with Housing Bearing / Pillow Block Bearing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sanitary Stainless Steel SS304/316L Manual Weld/Clamp/Thread Butterfly Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Milling Precision Stainless Steel CNC Machinery Parts]

Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Road Safety Warning Barricade LED Solar Powered Blinking Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter]

Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2 Round Sash Pulley for Chain - Brass (PVD)]

Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Manufacturer Customized Plastic Injection Molding, Part I.Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized 8 Layers Round LED Panel Aluminum PCB for LED Light]

Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine.60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light, 175-184[125kHz 13.56MHz UHF Chip Card Plastic RFID NFC Chip Card]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[33kv Dry Type Resin Casted Distribution Transformer]

Whitaker,J.R.(1963),Solar Energy Storad Power Generation System. Analytical Chemistry35(12), 1950-1953[Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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