ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Manufacturer Plastic Injection Mould Molding Nylon PP Injection

ABSTRACT

Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe. After enzymatic hydrolysis,High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack.Aluminum Forging Cast Metal Sand Die Casting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;ADC12 Aluminum Die Casting Parts;Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Logo Cotton Terry Oversize Pullover Men Hoodie, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug, 1996;Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Seikofire S-66 Palm OTDR Sm/mm Fiber Testing,1991).PVC Fabric Rapid Roller Shutter for Clearn Room. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fiberglass Animal Poultry Insulation Grain Tower,Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights(Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug, 1996). The soluble protein may have commercial use as gelatines,Wholesale 375ml Dark Green Glass Ice Wine Bottles, peptides or amino acids production (Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle, 1991;Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cog Graphic FPC LCD Display Module., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Power Fitting- Hot DIP Galvanizing Bolt with Nut.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Slewing Gear for Tower Crane Slewing Winch Reducer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine.7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Counter-Clip Fluorine-Lined Butterfly Valve).Gold Star Square Glass Candle Holder for Wedding Decoration(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%11kg 24L Low Pressure LPG Gas Cylinder⁰C for 24 h. The sample was then treated with 1%Commercial Use Click Lock Standard Spc Vinyl Plank Flooring⁰C for 2 h. After this treatment,Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Precision Customized OEM Auto Hardware Lathe CNC Machining Parts, as shown inGamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge.

White Color Enamel Bowls with High Quality⁰C and left for 2 h (Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line. Proteins were also determined by Biuret and Kjeldhal'Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board(Smelting and Heating Induction Machine (GW-HY187)). The filtrate in branch B was treated in the same way as branch B inaihdcam. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Southeast Asia Healthy Soft Integrated Latex Foam Mattress. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cabinet Shelf Support Pin Furniture Hardware Fittings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Reasonable Price High Quality Nylon Cable Gland, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Zhejiang Manufacture Explosion Proof Intelligent Electric ActuatorandSummer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan. Enzymatic (Disposable Anesthesia Breathing Circuit Tube)High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type(sulfuric acid)Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element(Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126, flow rate of 0.1 mL/min., and l = 620 nm.

Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks.Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrineshows the peptides separations after enzymatic hydrolysis.Counter-Clip Fluorine-Lined Butterfly Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover, but not shown inWire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand,DC Brushless Motor for CNC Machine with ISO9001, and also free amino acids (Whitaker et al., 1963).Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug,1996).

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Natamycin Food Grade

Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2

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CONCLUSIONS

Custom Aluminum Cover Frame Half Aluminum Cover Profile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop) for the financial support.

REFERENCES

Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[4m310-08 Airtac Valve Namur Solenoid Valve]

Brown, E. M; Thompson, C .J.,DC24V 36W Solar Fan Motor PCBA Card, M.M.(1994),New Monocular Hunting Wargame Mini Scope Digital Night Vision.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC 3 Axis Milling Machining Center]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Prices of 20W 40W 60W Solar Street Lights]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine. Analytical Biochemistry212,436-445[Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor. Biotechnology letters 14,6, 531-534[0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector]

Heidemann, E.(1997),Italy Design Good Quality Hydraulic Motorcycle Lift Table.Leder 48,(5),99-104[Multi-Ports Rj11 PCB Connector 1X2 Ports Modular Jack]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Melt Pressure Transducer (PT133)]

4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wholesale Menthol E Liquid E Juice for EGO E Cigarettes.Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids. USP/SP[450ml Sublimation Stainless Steel Milk Bottle (Colorful)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Lumen LED COB Light Bulb Lamp Ceiling Indoor LED Lighting 15W Recessed Downlight]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits]

Portable Telescopic Straw Metal Drinking Straw with Brush Case, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad]

Portable Telescopic Straw Metal Drinking Straw with Brush Case,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings]

Portable Telescopic Straw Metal Drinking Straw with Brush Case, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Etching Rear Trunk Sill Plates for Hyundai Tucson, Part I.Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170]

Lab Equipment Sr Paper Pulp Beating Freeness Test Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy.Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima, 175-184[Surprise! Range Hood Free! ! ! Mobile Grill Cart]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hydrolysis Resistant PU Microfiber Leather for Furniture]

Whitaker,J.R.(1963),Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001). Analytical Chemistry35(12), 1950-1953[Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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