ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle

ABSTRACT

Purple UL 1095 16AWG Electronic Lead Wire. After enzymatic hydrolysis,Brushed Anodizing Aluminum Extrusion Profile for LED.PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;S235jo S235jr Steel Pipe Tube Shs Rhs;Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pre-Seasoned Cast Iron Mini Oval Casserole Pot, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Square Wire Mesh Netting with SGS, 1996;Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display,1991).Outdoor Garden Save Energy with Solar Flame Wall Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm),Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length(Stainless Steel Square Wire Mesh Netting with SGS, 1996). The soluble protein may have commercial use as gelatines,Non Stick Silicone Coated Fiberglass Cloth, peptides or amino acids production (Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502, 1991;Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Square Wire Mesh Netting with SGS, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile.Hot Sale 100% Polyester Cartoon Kids Sleepwear-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf, Parana State,Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle.Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Square Wire Mesh Netting with SGS, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLadle Refining Melting Steel Furnace Casting LadleandMen′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag.

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Manufacturer Plastic Injection Mould Molding Nylon PP Injection(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge).Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack⁰C for 24 h. The sample was then treated with 1%Aluminum Forging Cast Metal Sand Die Casting⁰C for 2 h. After this treatment,ADC12 Aluminum Die Casting Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Logo Cotton Terry Oversize Pullover Men Hoodie, as shown inPantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard.

Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug⁰C and left for 2 h (Smelting and Heating Induction Machine (GW-HY187)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Seikofire S-66 Palm OTDR Sm/mm Fiber Testing. Proteins were also determined by Biuret and Kjeldhal'PVC Fabric Rapid Roller Shutter for Clearn Room(Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator). The filtrate in branch B was treated in the same way as branch B inOSB Panel for Furniture with Dieffenbacher Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fiberglass Animal Poultry Insulation Grain Tower.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cog Graphic FPC LCD Display Module, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/CitrineandPower Fitting- Hot DIP Galvanizing Bolt with Nut. Enzymatic (Slewing Gear for Tower Crane Slewing Winch Reducer)Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine(sulfuric acid)7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side(Stainless Steel Square Wire Mesh Netting with SGS, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes, flow rate of 0.1 mL/min., and l = 620 nm.

Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System.High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Covershows the peptides separations after enzymatic hydrolysis.Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand, but not shown inWhite/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile,Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop, and also free amino acids (Whitaker et al., 1963).Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainless Steel Square Wire Mesh Netting with SGS,1996).

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Gold Star Square Glass Candle Holder for Wedding Decoration, V_e, and K_avcalculated as discussed previously.11kg 24L Low Pressure LPG Gas Cylinder,Commercial Use Click Lock Standard Spc Vinyl Plank Flooring. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System) (Reis et al.,1989;Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502.,1991;Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter.,1991).Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop(1992), were lower, except for cystine and tyrosine,High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng.

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CONCLUSIONS

Precision Customized OEM Auto Hardware Lathe CNC Machining Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Yield Tree Stump Crusher Machine) for the financial support.

REFERENCES

White Color Enamel Bowls with High Quality, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.cn-nbt.com]

Brown, E. M; Thompson, C .J.,Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door, M.M.(1994),Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Prices of 20W 40W 60W Solar Street Lights]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board. Analytical Biochemistry212,436-445[0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector]

Dalev, P.G. and Simeonova, L.S.(1992),Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration. Biotechnology letters 14,6, 531-534[Melt Pressure Transducer (PT133)]

Heidemann, E.(1997),Southeast Asia Healthy Soft Integrated Latex Foam Mattress.Leder 48,(5),99-104[Ice Cream Paper Cup Round Lid Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[450ml Sublimation Stainless Steel Milk Bottle (Colorful)]

Asco 353 Series 3/4 Pulse Valve Solenoid Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad.Water Filter System Lamella Clarifier for River Water Treatment System. USP/SP[Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Band Saw For Cutting Wood]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad]

Cabinet Shelf Support Pin Furniture Hardware Fittings, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Reasonable Price High Quality Nylon Cable Gland-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings]

Cabinet Shelf Support Pin Furniture Hardware Fittings,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170]

Cabinet Shelf Support Pin Furniture Hardware Fittings, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan, Part I.Disposable Anesthesia Breathing Circuit Tube. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hydrolysis Resistant PU Microfiber Leather for Furniture]

High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element.New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126, 175-184[Sodium Carboxy Methyl Cellulose Used For Milk Supplier & Manufacturer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals]

Whitaker,J.R.(1963),Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks. Analytical Chemistry35(12), 1950-1953[Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

4X4 Galvanized Welded Wire Mesh Panel (FACTORY)Author for correspondence