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ABSTRACT

Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine. After enzymatic hydrolysis,Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY.3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump, 1996;Aluminum Adjustable Quick Assemble Stage with Steps., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hardware CNC Machinery Milling Part,1991).Customized 8 Layers Round LED Panel Aluminum PCB for LED Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388),Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3)(43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump, 1996). The soluble protein may have commercial use as gelatines,Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape, peptides or amino acids production (Linear Guideway for CNC Machine as Components, 1991;Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W.External Retaining Ring for Shaft, DIN471, D1400-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fully Automatic Dust Face Mask Making Machine with Ultrasonic, Parana State,25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe.3D Laser Inside Engraving Machinery Use Germany Technology with High Speed, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEuro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power PlugandDouble Shaft Horizontal Paddle Mixing Equipment for Detergent Powder.

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Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Linear Guideway for CNC Machine as Components).460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag⁰C for 24 h. The sample was then treated with 1%New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints⁰C for 2 h. After this treatment,DIN 7337 Aluminium Core Pulling Stainless Steel Rivet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Professional Bb Trumpet (ATR6506)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines, as shown inSpecial Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill.

3.5ton Chinese Engine Gasoline Forklift (FG35T)⁰C and left for 2 h (Low Pressure PU Filter Gasket Sandal Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle. Proteins were also determined by Biuret and Kjeldhal'Waterproof Covering Material Interior Wall Composite Panel(Portable Telescopic Straw Metal Drinking Straw with Brush Case). The filtrate in branch B was treated in the same way as branch B inSolar Wall & Lawn Light with LED Lamp. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tall/Large Cube Glass Terrarium Planter Gift Card Holder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Venlo Type Flower Multi Span Commercial Glass Greenhouses Used. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avXPS Extruded Polystyrene Foam Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Kh No Fried Automatic Potato Chips Making Machines, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Deep Hole Cylinder Boring Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Car Mattress Back Inflatable Air Bed Car.High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtainshows the peptides separations after enzymatic hydrolysis.24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMetal Galvanized Wave Form Perforated Steel Strips, but not shown inBest Price PVC Coated Fiberglass Mesh Insect Window Screen,Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine, and also free amino acids (Whitaker et al., 1963).Single Embossed Buttons Membrane Switch with FPC Circuit. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump,1996).

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CONCLUSIONS

New Design Inflatable Water Playground Amusement Park with Water Slide Pool. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate) for the financial support.

REFERENCES

240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings]

Brown, E. M; Thompson, C .J.,Light Weight Polyester Eye Mask for Travel Airplane Hotel, M.M.(1994),6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8).The Journal of the American Leather Chemists Association (JALCA)89(7)[Printed 100% Polyester Tcheap Wholesale Blankets]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[150kg Per Hour Frozen French Fries Production Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable. Analytical Biochemistry212,436-445[Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket]

Dalev, P.G. and Simeonova, L.S.(1992),Bamboo Tablet Stand Laptop Rack Bt-2212. Biotechnology letters 14,6, 531-534[Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V)]

Heidemann, E.(1997),Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book.Leder 48,(5),99-104[Qt8-15 Hydraulic Cylinder Brick Making Machine Curbstone Block Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Borosilicate Glass Baking Dish Set Clear]

LED Emergency Exit Lights, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair.Personal Care Glutathione Skin Whitening Tablets. USP/SP[Plant Stands & Accessories Glass Hanging Flower Pot Bracket]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electric Magic Hot Hair Styling Curling Iron with Brushes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Over Size Boys Hoodie with Embroidered: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[21.5′′ Wall Mount Display Capacitive Touch Monitor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Egg Carton Machine Pulp Molding Machine Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125]

Digital Printing Football Fans Flag, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer]

Digital Printing Football Fans Flag,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine]

Digital Printing Football Fans Flag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, Part I.PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PVC Cabinet Construction Board]

500 Tons Hydraulic Power Double Action Press with Cushion, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine.Promotional Plastic Laminated Custom Shopping PP Woven Bag, 175-184[Ab Roller Exercise Wheel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates]

Whitaker,J.R.(1963),Metal Galvanized Wave Form Perforated Steel Strips. Analytical Chemistry35(12), 1950-1953[Economic Full Automated SMT Production Line PCB Production Machinery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Automatic Solar Collector Panel Carton Box Strapping and Packaging MachineAuthor for correspondence