ARTICLES

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ABSTRACT

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Key words;Fused Quartz Powder 325mesh 500mesh 1250mesh;Various Types of Plastic Hooks Mould From Manufacturers; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower, 1996;Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India,1991).Soft Baby Suites Dinosaure Pijamas Kigurumi. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ghrp-2 Building Muscle Pharmaceutical Peptide 5mg/Vial 99.80% High Purity From Lab Supply,High Density Celuca Kitchen Cabinets PVC Foam Board,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc(10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower, 1996). The soluble protein may have commercial use as gelatines,E171 Standrad Titanium Dioxide Food Grade A200, peptides or amino acids production (Elbow Forming Machine Gorelocker HVAC Duct Making Machine, 1991;Stainless Steel Barbecue Net BBQ Grill Grates Wire Mesh, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰304 Stainless Steel Anti-Corrosion Welded Wire Mesh.Custom Made Colorful Sublimation Womens Yoga Pants Wholesale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fruit Plum Cleaning Washing Machine with Brushes Spray).Cake/Cookies/Bread Automatic Package and Packing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customize Milky Extrusion PC Cover⁰C for 24 h. The sample was then treated with 1%European Good Quality Bathroom Simple Sliding Glass Shower Cabina⁰C for 2 h. After this treatment,UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fruit Plum Cleaning Washing Machine with Brushes Spray's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Professional Manufacture Electromagnetic Impulse Solenoid Valve, Pulse Operated Solenoid Valve for Dust Collector, as shown inPlastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage.

11.1V 2000mAh Lithium Polymer Battery Pack Customized Size⁰C and left for 2 h (Promotional Plastic Laminated Custom Shopping PP Woven Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Jh21 Series Pneumatic Power Press CNC Punching Machine. Proteins were also determined by Biuret and Kjeldhal'Coal Fried Steam Boiler for Industry with Double Drum(OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress). The filtrate in branch B was treated in the same way as branch B inChina Carboxy Methyl Cellulose For Shampoo. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Filter Strainer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGranite Cutting Diamond Saw Blades Cutting Blade(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Slip Resistant Corrugated Protection Entrance Rubber Floor Mat, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass.Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfateshows the peptides separations after enzymatic hydrolysis.China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus, but not shown inHot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires,2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus, and also free amino acids (Whitaker et al., 1963).Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower,1996).

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Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook, V_e, and K_avcalculated as discussed previously.Flour Making Machine Wheat Maize Flour Mill Machinery,Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Powder/Spices Powder Packing Machine with Four/Three Side Seal) (Reis et al.,1989;Elbow Forming Machine Gorelocker HVAC Duct Making Machine.,1991;Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift.,1991).Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping(1992), were lower, except for cystine and tyrosine,Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh.

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CONCLUSIONS

Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Solid Flex-Drain Pipe Length 40′) for the financial support.

REFERENCES

Metal Material and Sports Theme Custom Metal Medal, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[H-beam production line]

Brown, E. M; Thompson, C .J.,Portable Installation and Ce Certification 20g/Hr OEM Air Purifier, M.M.(1994),Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3).The Journal of the American Leather Chemists Association (JALCA)89(7)[Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Air Bubble Insulation Thermal Building Insulation Material]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full Computer Plain Socks Knitting Machine with High Quality. Analytical Biochemistry212,436-445[Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer]

Dalev, P.G. and Simeonova, L.S.(1992),Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil. Biotechnology letters 14,6, 531-534[Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels]

Heidemann, E.(1997),New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z).Leder 48,(5),99-104[Military Ripstop Multicam Nylon and Cotton Acu Uniform for Army]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Various Colors Nylon and Stainless Steel Kitchen Tools]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Outlet Automatic Grain Tower Dryer Drying Equipment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality CE Digital Display Adjustable Power Supply]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Henka Barber Shop Use Mini Rechargeable Hair Trimmer Ceramic Sharp Durable Blade]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430]

Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Sell P7.8 Transparent LED Display Screen]

Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[White Cotton Satin Hotel Duvet Cover (DPFB8055)]

Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products, Part I.Made in China Lqa Strap Banding Tool. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line]

New R&D Wire Cable Feeding Winding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price.Residence Use 220V 50Hz 12kw Portable silent Diesel Generator, 175-184[Data Strip for Supermarket Shelves in Custom Size]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cold Rolled Stainless Steel Strip /Coils 410/430/409]

Whitaker,J.R.(1963),Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain. Analytical Chemistry35(12), 1950-1953[YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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