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ABSTRACT

Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine. After enzymatic hydrolysis,PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use.Blue Steel Packing Strip. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Powder and Wax Brush with Wooden Handle;Cheap Price Paper Straws Individually Wrapped Drinking Straw; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF, 1996;Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Smelting and Heating Induction Machine (GW-HY187),1991).Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Home Products 16′ Perforated Flex Pipe,1220 Fork Length 3-Wheel Electric Forklift Truck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits(Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF, 1996). The soluble protein may have commercial use as gelatines,FM USB Radio Audio Power Amplifier for Home Bar Club, peptides or amino acids production (Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable, 1991;Hot Sell Mechanical Bar Screen for Water Treatment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Glow Powder for Luminous Coating., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.700 mm RP HP UHP Graphite Electrode Sold at Factory Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Union Metal Steel Building and Construction Material.Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:450ml Sublimation Stainless Steel Milk Bottle (Colorful)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Air Cooled Split Type Ducted Air Conditioner AC Unit, Parana State,Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System.Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping MallandChicken Cage Mesh Hexagonal Wire Mesh Machine.

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High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Roller Wedge Screen Cylinder).Gym Fitness Equipment Adjustable Weight Bench(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Sell P7.8 Transparent LED Display Screen⁰C for 24 h. The sample was then treated with 1%Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V)⁰C for 2 h. After this treatment,Original Weichai Engine Truck Spare Parts Valve Cap 61500050105. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sliding Panel Saw Heavy Duty CNC Band Saw for Furniture Ban Resaw Machine for Lumber, Round Log Slinding Sawmill, Band Resaw for Lumber's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim, as shown inFreshwater Pearl Butterfly Flower Bride Hairpin.

Charms for Jewelry Making (Sold Per Bag 10PCS Inside)⁰C and left for 2 h (Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fused Quartz Powder 325mesh 500mesh 1250mesh. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Various Types of Plastic Hooks Mould From Manufacturers. Proteins were also determined by Biuret and Kjeldhal'New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle(Elbow Forming Machine Gorelocker HVAC Duct Making Machine). The filtrate in branch B was treated in the same way as branch B inwww.orist-fashion.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWaste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ghrp-2 Building Muscle Pharmaceutical Peptide 5mg/Vial 99.80% High Purity From Lab Supply⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Density Celuca Kitchen Cabinets PVC Foam Board, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric.Customize Milky Extrusion PC Covershows the peptides separations after enzymatic hydrolysis.304 Stainless Steel Anti-Corrosion Welded Wire Mesh(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAnti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair, but not shown inVertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine,Custom Made Colorful Sublimation Womens Yoga Pants Wholesale, and also free amino acids (Whitaker et al., 1963).Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF,1996).

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CONCLUSIONS

Customize Milky Extrusion PC Cover. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3.0kw Spindle CNC Router with Certificate for Woodworking) for the financial support.

REFERENCES

European Good Quality Bathroom Simple Sliding Glass Shower Cabina, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Car Air Outlet Perfume Bottle Empty Air Conditioner Perfume Holder Creative Car Air Outlet Bottle Car Hanging 8ml]

Brown, E. M; Thompson, C .J.,UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, M.M.(1994),Fruit Plum Cleaning Washing Machine with Brushes Spray.The Journal of the American Leather Chemists Association (JALCA)89(7)[6 Inch Plastic Breathing Cap for The IBC Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Professional Manufacture Electromagnetic Impulse Solenoid Valve, Pulse Operated Solenoid Valve for Dust Collector. Analytical Biochemistry212,436-445[China Customized Chilled Cast Iron Roll]

Dalev, P.G. and Simeonova, L.S.(1992),11.1V 2000mAh Lithium Polymer Battery Pack Customized Size. Biotechnology letters 14,6, 531-534[Water Slide for Indoor Play Structure]

Heidemann, E.(1997),Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677.Leder 48,(5),99-104[Oxygen Cylinder Filling Compressor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate]

Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Jh21 Series Pneumatic Power Press CNC Punching Machine.Coal Fried Steam Boiler for Industry with Double Drum. USP/SP[Tct Circular Saw Blade for Grass Cutting]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Friewood PP Leno Sack]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe]

Stainless Steel Filter Strainer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Granite Cutting Diamond Saw Blades Cutting Blade-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Metal Gold Marathon Running Sport Medal for Promotion Gift]

Stainless Steel Filter Strainer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator]

Stainless Steel Filter Strainer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Steel Forged Automobile Spare Parts with Customized Drawing, Part I.Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management]

Slip Resistant Corrugated Protection Entrance Rubber Floor Mat, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp.T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm), 175-184[Powder Mixing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mesh Bag Packing 20kg Pure White Garlic]

Whitaker,J.R.(1963),High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine. Analytical Chemistry35(12), 1950-1953[New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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