ARTICLES

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ABSTRACT

Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve. After enzymatic hydrolysis,Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine.60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Aluminum Cover Frame Half Aluminum Cover Profile, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, 1996;DC24V 36W Solar Fan Motor PCBA Card., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Monocular Hunting Wargame Mini Scope Digital Night Vision,1991).Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor,Italy Design Good Quality Hydraulic Motorcycle Lift Table,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Menthol E Liquid E Juice for EGO E Cigarettes(Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, 1996). The soluble protein may have commercial use as gelatines,Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids, peptides or amino acids production (New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9, 1991;Portable Telescopic Straw Metal Drinking Straw with Brush Case, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Etching Rear Trunk Sill Plates for Hyundai Tucson.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Lab Equipment Sr Paper Pulp Beating Freeness Test Machine.CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070), Parana State,Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08).Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAluminum Can Automatic Juice Beer Soda Filling Packing Canning MachineandLow Cost Safety Guarantee Storage Silo with High-Efficient Conveyors.

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Ladle Refining Melting Steel Furnace Casting Ladle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ladle Refining Melting Steel Furnace Casting Ladle).Lishui Factory High Quality Metal Rolling Ball Screw(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Y-Spring Threaded Check Valve, 800 Wog⁰C for 24 h. The sample was then treated with 1%OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part⁰C for 2 h. After this treatment,Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Galvanized Eye Grab Bend Hook's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Transparent LED Display P3.91 Glass Wall LED Screen, as shown inMen′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag.

Surgical Power Tool Medical Care Orthopedic Mini Bone Saw⁰C and left for 2 h (Counter-Clip Fluorine-Lined Butterfly Valve). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Micro Small Plastic Product Injection Molding Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Glass Lifting Machine with Sucker for Glass Movements. Proteins were also determined by Biuret and Kjeldhal'Anodized Gold Brush Finish Aluminum Sheet Coil(Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge). The filtrate in branch B was treated in the same way as branch B inWater Pouch Filling Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Popular Industrial Sewing Quilting Machine With CO.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAlloy 6061 T4 T6 T651 Aluminum / Aluminium Plate(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Colorful 100% Cotton Hotel Face Towels, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Black Red Open Net Spandex Nylon Sexy Lingerie.Smelting and Heating Induction Machine (GW-HY187)shows the peptides separations after enzymatic hydrolysis.7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inZhejiang Manufacture Explosion Proof Intelligent Electric Actuator, but not shown inFashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine,Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor, and also free amino acids (Whitaker et al., 1963).LED Emergency Exit Lights. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility,1996).

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Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY, V_e, and K_avcalculated as discussed previously.3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope,Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (94V0 Circuit Board Mobile Phone PCB Motherboard) (Reis et al.,1989;New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9.,1991;DC24V 36W Solar Fan Motor PCBA Card.,1991).Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag(1992), were lower, except for cystine and tyrosine,Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels.

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CONCLUSIONS

43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Double Roller Rotary Wood Peeling Machine with CE for Sale) for the financial support.

REFERENCES

Aluminum Adjustable Quick Assemble Stage with Steps, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Diecast Aluminium Part]

Brown, E. M; Thompson, C .J.,Hardware CNC Machinery Milling Part, M.M.(1994),Customized 8 Layers Round LED Panel Aluminum PCB for LED Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Really Microfiber PVC Leather for Sofa Furniture]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388). Analytical Biochemistry212,436-445[CNC 3 Axis Milling Machining Center]

Dalev, P.G. and Simeonova, L.S.(1992),Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge. Biotechnology letters 14,6, 531-534[Wholesale Prices of 20W 40W 60W Solar Street Lights]

Heidemann, E.(1997),Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3).Leder 48,(5),99-104[Up Acting Hydraulic Press]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine]

Road Safety Warning Barricade LED Solar Powered Blinking Light, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape.Linear Guideway for CNC Machine as Components. USP/SP[0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Melt Pressure Transducer (PT133)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[450ml Sublimation Stainless Steel Milk Bottle (Colorful)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Iposter P25]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field]

40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard]

40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits]

40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch, Part I.LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad]

External Retaining Ring for Shaft, DIN471, D1400, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber.Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand, 175-184[Cutting Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings]

Whitaker,J.R.(1963),Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine. Analytical Chemistry35(12), 1950-1953[Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hydrolysis Resistant PU Microfiber Leather for FurnitureAuthor for correspondence