ARTICLES

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ABSTRACT

Laptop Bag College Men Backpack Leather Back Pack Bags. After enzymatic hydrolysis,Pet Dog Grooming Rake Dematting Tool Shedding Comb.Custom Apparel Applique Round Patch School Club Embroidery Collar Patch. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Food Metal Detector Machine for Production Line, 1996;Multi-Functional Backpack Manual 52cc Brush Cutter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa,1991).Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm,White Cotton Satin Hotel Duvet Cover (DPFB8055),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Small Cleaning Brush ESD Anti Static Brush ESD Brush Black(Automatic Food Metal Detector Machine for Production Line, 1996). The soluble protein may have commercial use as gelatines,PAC /Poly Aluminium Chloride for Water Treatment, peptides or amino acids production (Aluminum Sheet Shearing Machine, 1991;Electrical PV DC Solar Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tomato Bubble and High Pressure Washing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Upholstery Ribbon Christmas Tape.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Food Metal Detector Machine for Production Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2+1 Flavors Soft Ice Cream Machine with Pre-Cooling.Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Modern 4 Person Workstation with Computer Desk Modular Office Furniture(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Medical Human Body Thermometer).High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Women Fashion Winter V Neck Long Sleeve Clothes⁰C for 24 h. The sample was then treated with 1%Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line⁰C for 2 h. After this treatment,3D CNC Router for Wood Cutting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Screens / Wedge Wire Filter Element's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Amazon Armor TPU Silicone Phone Case for Honor 10 Lite, as shown in35 Degree Escalator with Auto Start Function in Parallel.

EPS Foam Sheet Extrusion Machine for Fast Food Box Making⁰C and left for 2 h (CH Series Pid Intelligent Temperature Controller CH402). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah. Proteins were also determined by Biuret and Kjeldhal'30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light(Fashion Metal Button Crystal Shank Button for Garment). The filtrate in branch B was treated in the same way as branch B inOral Liquid Filling Production Line. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Stainless Steel Plate Flange(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Easy Operational Cup Cake Filling Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Folding Play Mat Crawl Play Mat, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Humidity Refrigeration Digital Controller Jsd-100+) for the financial support.

REFERENCES

Adhesive CAS 9003-35-4 Phenolic Resin, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stock Children Bikes]

Brown, E. M; Thompson, C .J.,Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop, M.M.(1994),Manufacture Best Choice Liquid Flocculant for Ink Removal.The Journal of the American Leather Chemists Association (JALCA)89(7)[Widely Application Latest Designed Coffee Capsule Filling Packing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Dipped Galvanized Oval Wire Brazil. Analytical Biochemistry212,436-445[Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem]

Dalev, P.G. and Simeonova, L.S.(1992),Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys. Biotechnology letters 14,6, 531-534[Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf]

Heidemann, E.(1997),Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film.Leder 48,(5),99-104[Multi Touch Interactive Smart Board Supplier & Factory]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ss Reduced Port 2000wog 1PC Screwed Ball Valve]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1jo4 Hydraulic Adaptors for Hose Fittings]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminum Foil PE Bottle Cap Induction Seal Liner: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Super Strong Neodymium Fishing Magnets]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low]

Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sophisticated Durable Membrane Panel/Panel with 3m Glue-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film]

Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electric Fireplace PCBA with Double Layers Circuit Board]

Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder, Part I.HDPE Polyethylene PE Pipe for Water Supply. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic Injection Moulding Machine Supplier / Factory /Manufacturer]

Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door.Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station, 175-184[Scissors Grass Hedge Trimmer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder]

Whitaker,J.R.(1963),Galvanized Pressure Locked Grating. Analytical Chemistry35(12), 1950-1953[Whole Set Gas Spring Strut for Furniture]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Union Metal Steel Building and Construction MaterialAuthor for correspondence