ARTICLES

Titanium Dioxide Anatase for Paper Making

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Pipe Thermostat with Sensor Ce Certificate. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Walking Long Distance Surveying Instrument Meter Measuring Wheel

ABSTRACT

Chinese Supplier of PVC Profile. After enzymatic hydrolysis,Tianjin Manufacturer PTFE Coated Butterfly Valve.Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag, 1996;VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ALS Stage 5W RGB Animation Laser Light,1991).Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light,Pet Anti-Static Needled Mat,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine(Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag, 1996). The soluble protein may have commercial use as gelatines,OEM Sheet Metal Fabrication for Aluminum Parts, peptides or amino acids production (Hwf Tsp Core Bit for Geotechnical Drilling, 1991;Price of Automatic Sludge Dewatering Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Worm Gear Operation Wafer Lined F3 Butterfly Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag.Positive Drive Turn Belt for Spiral Freezing Equipment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Chinese Herb Medicine Angelica Root Extract(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Whole Set Gas Spring Strut for Furniture(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM).Dia. 5.5mm Bonding ND: YAG Laser Rod Lens(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CNC Machining Turning Anodized Aluminum Worm Gear⁰C for 24 h. The sample was then treated with 1%China Solar Panel Solar Cell Photovoltaic 300W Hot Sell⁰C for 2 h. After this treatment,Vacuum Coating Machine Water Chiller (CW-6300AN). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Planter Moving Round Planter Saucer Casters Plant Trolley (M)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China, as shown inCustomized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift.

Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)⁰C and left for 2 h (Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CNC Machining Non-Standard Hardware Parts. Proteins were also determined by Biuret and Kjeldhal'DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb(Spare Parts for Caterpillar Construction Machinery). The filtrate in branch B was treated in the same way as branch B inCctv Rj11 Telephone Patch Panel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Galvanized Solar System Mounting Bracket Connector.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Digital Programmable Ntc Temperature Controller. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWater Meter Spare Parts Rubber Rings for Sealing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Road Construction Fabric Non-Woven Geotextile 200GSM⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Polymer Modified Cement Based Elastic Waterproof Coating, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO TrucksandBiodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lidsshow the gel chromatographic separation of soluble protein with Sephadex G-Home Machine 2019 New Commercial Treadmill Gym Equipment.Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card, and another peak at 45 mL not shown inHigh Quality Welding Structure, for peptides lower than 3000 Da.Residential Reverse Osmosis 0.0001um Water Filter Water Filtration(Condell et al.,1993) .

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CONCLUSIONS

OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Original Testo 174t Mini Temperature Data Logger No. 0572 1560) for the financial support.

REFERENCES

Wood Veneer Peeling and Clipping Combined Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Amber Glass Storage Jar]

Brown, E. M; Thompson, C .J.,Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone, M.M.(1994),Wholesale High Quality Decoration Oil Painting, Home Decoration Painting, Art Painting (abstract sailing oil painting).The Journal of the American Leather Chemists Association (JALCA)89(7)[FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Si3n4 Full Ceramic  Bearing  608 Size 8X22X7. Analytical Biochemistry212,436-445[8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light]

Dalev, P.G. and Simeonova, L.S.(1992),32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall. Biotechnology letters 14,6, 531-534[Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe]

Heidemann, E.(1997),Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker.Leder 48,(5),99-104[Bronzing Roller]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Diameter 1300mm Lifting Electro Magnet for Cast Iron]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Car Air Conditional Compressor Magetic Clutch 5pk: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[43 Glass Beads Cat Eye Aluminum Road Stud]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Metal Police Name School Button Military Pin Badge]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Water Cooled Low Temperature Screw Chiller for Industrial Workshop]

High Quality Engieered Veneer Faced Commericial Plywood, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hex Thin Head Nut-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Prefab Steel Building for Garment Factory]

High Quality Engieered Veneer Faced Commericial Plywood,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag]

High Quality Engieered Veneer Faced Commericial Plywood, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2, Part I.Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Glass Lifting Machine with Sucker for Glass Movements]

Spare Parts for Caterpillar Construction Machinery, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank.50 Grams Ozone Producing Device for Makeup Toner Producing Plant, 175-184[Luxury Pvdf Building Roof]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Flat Car Sun Roof Tent Awning]

Whitaker,J.R.(1963),Flat Pack Temperary Container House 20FT 40FT. Analytical Chemistry35(12), 1950-1953[Atf-805 Auto Car Transmission System Flushing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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