ARTICLES

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ABSTRACT

Stainless Steel Mechanic Parts for Lock Accessories with MIM Process. After enzymatic hydrolysis,High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT.Mining Machinery Sand Vibrating Sieve for River Sand. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Palet Coil Nail Welding Machine/Nail Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, 1996;High Quality MPO Singlemode Duplex Fiber Optic Adapter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hotel Bedding,1991).Supplier V Wedge Wire Johnson Water Well Screen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles,10A MPPT Solar Controllers PV 60V for off-Grid,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears(Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, 1996). The soluble protein may have commercial use as gelatines,Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole, peptides or amino acids production (60ml 100ml Customized Glass Jar for Spice, Candle, Candy, 1991;Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Outlet Automatic Grain Tower Dryer Drying Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mesh Bag Packing 20kg Pure White Garlic. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Supreme 524 Mv Sea Kayak.Trunk Organizer Foldable Water Proof-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:100%Polyester Burnout Velvet Sofa Fabric (pineapple grid)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Diesel Generator Parts AVR Ki-Davr-95s for KiporAlcalase2.5 L DX,Gld Brand Padlock Brass Popular in 5 Continents  , with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-No Lead Bronze Flange Manufacturer); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Snsc Fd30 Side Shift New Diesel Forklift 3ton, Parana State,Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500.Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCar Mattress Back Inflatable Air Bed CarandDecorative Aluminum Solid Panel for Commercial Building.

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Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (35 Degree Escalator with Auto Start Function in Parallel).Laptop Bag College Men Backpack Leather Back Pack Bags(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pet Dog Grooming Rake Dematting Tool Shedding Comb⁰C for 24 h. The sample was then treated with 1%Custom Apparel Applique Round Patch School Club Embroidery Collar Patch⁰C for 2 h. After this treatment,Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror, as shown inCH Series Pid Intelligent Temperature Controller CH402.

Automatic Food Metal Detector Machine for Production Line⁰C and left for 2 h (Fashion Metal Button Crystal Shank Button for Garment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Multi-Functional Backpack Manual 52cc Brush Cutter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa. Proteins were also determined by Biuret and Kjeldhal'Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine(High-End Business Magnet Leather Ring Binder Notebook). The filtrate in branch B was treated in the same way as branch B indc solar wire. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%White Cotton Satin Hotel Duvet Cover (DPFB8055). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSmall Cleaning Brush ESD Anti Static Brush ESD Brush Black(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum Sheet Shearing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electrical PV DC Solar Cable, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Tomato Bubble and High Pressure Washing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Automatic 200-800kg Butterfly Chicken Breast Meat Slicing MachineandUpholstery Ribbon Christmas Tape. Enzymatic (Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate)2+1 Flavors Soft Ice Cream Machine with Pre-Cooling(sulfuric acid)Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver(Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse, flow rate of 0.1 mL/min., and l = 620 nm.

Best Sale Low Price Household Air freshener Spray.Lower Noise Colled Rolled Ball Screwshows the peptides separations after enzymatic hydrolysis.Factory Supplied High Strength Marine Anchor Chain(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGalvanized Eye Grab Bend Hook, but not shown inChinese Galvanized Rectangular Tube for Buildings Materials,Humidity Refrigeration Digital Controller Jsd-100+, and also free amino acids (Whitaker et al., 1963).Elegant Europe Dessign Pillow Top Mattress. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine,1996).

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PVC Connector Sleeve Soft Plastic Battery Terminal CoversandFactory Price DIN5685 Galvanized Marine Anchor Link Chainshow the gel chromatographic separation of soluble protein with Sephadex G-8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8).Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts, and another peak at 45 mL not shown inVertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C), for peptides lower than 3000 Da.Modern 4 Person Workstation with Computer Desk Modular Office Furniture(Condell et al.,1993) .

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High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill, V_e, and K_avcalculated as discussed previously.Women Fashion Winter V Neck Long Sleeve Clothes,Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Supply Perforated Metal Mesh) (Reis et al.,1989;60ml 100ml Customized Glass Jar for Spice, Candle, Candy.,1991;High Quality MPO Singlemode Duplex Fiber Optic Adapter.,1991).3D CNC Router for Wood Cutting Machine(1992), were lower, except for cystine and tyrosine,Stainless Steel Screens / Wedge Wire Filter Element.

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CONCLUSIONS

Amazon Armor TPU Silicone Phone Case for Honor 10 Lite. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (No Lead Bronze Flange Manufacturer) for the financial support.

REFERENCES

EPS Foam Sheet Extrusion Machine for Fast Food Box Making, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Best Price Frequency Converter]

Brown, E. M; Thompson, C .J.,Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe, M.M.(1994),Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah.The Journal of the American Leather Chemists Association (JALCA)89(7)[Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light. Analytical Biochemistry212,436-445[Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf]

Dalev, P.G. and Simeonova, L.S.(1992),Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel. Biotechnology letters 14,6, 531-534[Ss Reduced Port 2000wog 1PC Screwed Ball Valve]

Heidemann, E.(1997),YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine.Leder 48,(5),99-104[High Quality and Low Price Colourful Duct Tape]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Residential Reverse Osmosis 0.0001um Water Filter Water Filtration]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Easy Operational Cup Cake Filling Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Laser Cutting Metal Wall Art]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film]

Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Folding Play Mat Crawl Play Mat-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electric Fireplace PCBA with Double Layers Circuit Board]

Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Injection Moulding Machine Supplier / Factory /Manufacturer]

Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wooden Grain Perforated Panel for Metal Mesh Curtain Wall, Part I.Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder]

Folding  Platform Hand  Trolley , J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pump Flower Sprinkler with Automatic Watering System.Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant, 175-184[Mini Focus Modern Black Dial Men Quartz Wrist Watch with Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Whole Set Gas Spring Strut for Furniture]

Whitaker,J.R.(1963),Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field. Analytical Chemistry35(12), 1950-1953[Union Metal Steel Building and Construction Material]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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