ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture

ABSTRACT

New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. After enzymatic hydrolysis,Whole Set Gas Spring Strut for Furniture.Dia. 5.5mm Bonding ND: YAG Laser Rod Lens. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CNC Machining Turning Anodized Aluminum Worm Gear;China Solar Panel Solar Cell Photovoltaic 300W Hot Sell; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Vacuum Coating Machine Water Chiller (CW-6300AN), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Planter Moving Round Planter Saucer Casters Plant Trolley (M), 1996;1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X),1991).Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Machining Non-Standard Hardware Parts,DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Galvanized Solar System Mounting Bracket Connector(Planter Moving Round Planter Saucer Casters Plant Trolley (M), 1996). The soluble protein may have commercial use as gelatines,Digital Programmable Ntc Temperature Controller, peptides or amino acids production (Water Meter Spare Parts Rubber Rings for Sealing, 1991;Stainless Steel HK Series Needle Roller Bearing HK0408, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Road Construction Fabric Non-Woven Geotextile 200GSM., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Polymer Modified Cement Based Elastic Waterproof Coating.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Planter Moving Round Planter Saucer Casters Plant Trolley (M), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset.Fashion Shoulder Backpack Handbag School Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Temperature Alternative Stainless Steel Band in Different Package, Parana State,Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture.Water Cooled Low Temperature Screw Chiller for Industrial Workshop, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Planter Moving Round Planter Saucer Casters Plant Trolley (M), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGlazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass PanelandCorrugated Board Production Line.

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Home Machine 2019 New Commercial Treadmill Gym Equipment(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe).Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Residential Reverse Osmosis 0.0001um Water Filter Water Filtration⁰C for 24 h. The sample was then treated with 1%Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle⁰C for 2 h. After this treatment,China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Folding Round Picnic Caravan Table RV Picnic Table Camping Table's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits, as shown inSafe Durable Construction Scaffolding Plank for Building.

3HP Industrial Cooling System Water Cooled Chiller⁰C and left for 2 h (Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wood Veneer Peeling and Clipping Combined Machine. Proteins were also determined by Biuret and Kjeldhal'Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone(Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)). The filtrate in branch B was treated in the same way as branch B inWorkshop Presses. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale High Quality Decoration Oil Painting, Home Decoration Painting, Art Painting (abstract sailing oil painting).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Si3n4 Full Ceramic  Bearing  608 Size 8X22X7. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Corrugated Board Production Line⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Car Air Conditional Compressor Magetic Clutch 5pk, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Blue Steel Packing Strip. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Speed Cable Wire Winding Braiding Machine) for the financial support.

REFERENCES

High Quality Powder and Wax Brush with Wooden Handle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[European Roller Shutter]

Brown, E. M; Thompson, C .J.,Cheap Price Paper Straws Individually Wrapped Drinking Straw, M.M.(1994),ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF. Analytical Biochemistry212,436-445[100W 120W 150W Outdoor Solar LED Street Lighting]

Dalev, P.G. and Simeonova, L.S.(1992),Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose. Biotechnology letters 14,6, 531-534[PAC /Poly Aluminium Chloride for Water Treatment]

Heidemann, E.(1997),Smelting and Heating Induction Machine (GW-HY187).Leder 48,(5),99-104[Light Resistance Polyvinyl Alcohol (PVA) Fiber for Motar Concrete Reinforcement]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Galvanized Roller Shutter Window Torsion Springs]

Original Testo 174t Mini Temperature Data Logger No. 0572 1560, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem.Home Products 16′ Perforated Flex Pipe. USP/SP[Italy Design Good Quality Hydraulic Motorcycle Lift Table]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1220 Fork Length 3-Wheel Electric Forklift Truck: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Immersion Gold Fr4 Pcb]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve]

Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),FM USB Radio Audio Power Amplifier for Home Bar Club-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[White Ceramic Shot Glass for Sublimation Printing - 1.5oz]

Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Graphite Ring Good Lubrication]

Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable, Part I.Hot Sell Mechanical Bar Screen for Water Treatment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC System Air Duct Line5 Make Machine for HVAC]

Glow Powder for Luminous Coating, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),700 mm RP HP UHP Graphite Electrode Sold at Factory Price.Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck, 175-184[Compound Microscope Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Famous Brand Luxury Packaging Box Gift Box]

Whitaker,J.R.(1963),Union Metal Steel Building and Construction Material. Analytical Chemistry35(12), 1950-1953[Full-Auto CNC Cutting Machine for Architecture Glass Sheet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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