ARTICLES

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ABSTRACT

Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill. After enzymatic hydrolysis,96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood.Kingdomway Brand Vitamin a 1000. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine;High Speed Roll Plastic T-Shirt Bag Making Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Black Galvanized Head Stainless Steel Screw, 1996;PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (45*60cm Anti-Fatigue ESD Rubber Floor Mat,1991).Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Vinyl Glove in Work & Labor Glove,Low Energy Needed Compact Flour Mill Plant for Milling Wheat,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Nylon Weatherproof PA6 Strain Relief Cable Gland(Wholesale Black Galvanized Head Stainless Steel Screw, 1996). The soluble protein may have commercial use as gelatines,Steel Roller Embossed Paper 2019 New Design, peptides or amino acids production (Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil, 1991;Factory Price DIN5685 Galvanized Marine Anchor Link Chain, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Level Application Waterproof Plastic Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Black Galvanized Head Stainless Steel Screw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine.Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Water Slide for Indoor Play Structure(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L BladderAlcalase2.5 L DX,1220*2440mm Concrete Formwork black film faced plywood, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Washami Teeth Care for Whitening Cool Mint Toothpaste); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hotel Steel Digital Room Safes with Large LED Display).2019 Foam Concrete Machine/Foamed Concrete Block Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball⁰C for 24 h. The sample was then treated with 1%Yyh Series One Dimension Tumbling Mixer Mixing Machine⁰C for 2 h. After this treatment,ODU 14 Pin Industrial Circular Connector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Classical New Coming Plastic Female Sunglasses, as shown inMilitary Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder.

OEM Double Sided Rigid PCB Circuit Board Manufacturer⁰C and left for 2 h (Custom Logo Cotton Terry Oversize Pullover Men Hoodie). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Robot of World Robotics Competition. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Professional Rope/Cable/Wire Braiding Machine with Factory Price. Proteins were also determined by Biuret and Kjeldhal'Aluminum PCB Circuit Board SMD PCBA LED PCB(Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing). The filtrate in branch B was treated in the same way as branch B inChina Aoi Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Supplier Steel Machine Bellows Cover Telescopic Shield Cover(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Chewing Dog Food Snack Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/White Ceramic Shot Glass for Sublimation Printing - 1.5oz, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Promotional Gifts Custom Printed Silicone Wristband/Bracelet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Washami Teeth Care for Whitening Cool Mint Toothpaste) for the financial support.

REFERENCES

Polyglycolic Acid Absorable Suture, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Metal 4 Drawer Filing Cabinet Storage Cabinet]

Brown, E. M; Thompson, C .J.,No. 1062 20PCS Tools, M.M.(1994),Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant.The Journal of the American Leather Chemists Association (JALCA)89(7)[Portable Telescopic Straw Metal Drinking Straw with Brush Case]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminum Mould Shoe Mould Making CNC Milling Router Machine. Analytical Biochemistry212,436-445[High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain]

Dalev, P.G. and Simeonova, L.S.(1992),High Pressure Fitting Quick Coupling Hydraulic Plug. Biotechnology letters 14,6, 531-534[Metal Galvanized Wave Form Perforated Steel Strips]

Heidemann, E.(1997),High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover.Leder 48,(5),99-104[High Quality High Temperature Powder Coating Oven]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Price PVC Coated Fiberglass Mesh Insect Window Screen]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ 3FT 3000lm IP66 Approved]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Swim Shorts Grey Mens Board Shorts with Mesh Liner]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom High Torque Planetary Gearbox 6mm 12mm 24mm 32mm Power Transmission Speed Reducer for Car Marine Engine Motors Robot Small Gears]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800]

Black Color Hexagon Bolt with High Quality, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube]

Black Color Hexagon Bolt with High Quality,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Transparent Composite Fiberglass Roof Tiles]

Black Color Hexagon Bolt with High Quality, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price, Part I.Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine]

High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72).Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery, 175-184[Wicker Flower Planter with Handle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ with Handle Vegetable Oil Nonstick]

Whitaker,J.R.(1963),Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042). Analytical Chemistry35(12), 1950-1953[Printed 100% Polyester Tcheap Wholesale Blankets]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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