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ABSTRACT

Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits. After enzymatic hydrolysis,FM USB Radio Audio Power Amplifier for Home Bar Club.Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Sell Mechanical Bar Screen for Water Treatment;Glow Powder for Luminous Coating; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.700 mm RP HP UHP Graphite Electrode Sold at Factory Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck, 1996;Union Metal Steel Building and Construction Material., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller,1991).450ml Sublimation Stainless Steel Milk Bottle (Colorful). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Lower Noise Colled Rolled Ball Screw,Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3.0kw Spindle CNC Router with Certificate for Woodworking(Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck, 1996). The soluble protein may have commercial use as gelatines,800W Electric Motor Price Lawn Mower Motor Concrete Mixer, peptides or amino acids production (Air Cooled Split Type Ducted Air Conditioner AC Unit, 1991;Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Gym Fitness Equipment Adjustable Weight Bench.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Sell P7.8 Transparent LED Display Screen. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V).Original Weichai Engine Truck Spare Parts Valve Cap 61500050105-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sliding Panel Saw Heavy Duty CNC Band Saw for Furniture Ban Resaw Machine for Lumber, Round Log Slinding Sawmill, Band Resaw for Lumber(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Single Stage High Speed Fruit and Vegetable Pulping Machine).Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Soft Baby Suites Dinosaure Pijamas Kigurumi⁰C for 24 h. The sample was then treated with 1%Ghrp-2 Building Muscle Pharmaceutical Peptide 5mg/Vial 99.80% High Purity From Lab Supply⁰C for 2 h. After this treatment,High Density Celuca Kitchen Cabinets PVC Foam Board. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)E171 Standrad Titanium Dioxide Food Grade A200, as shown in460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%304 Stainless Steel Anti-Corrosion Welded Wire Mesh. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Made Colorful Sublimation Womens Yoga Pants Wholesale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Fashion PVC Sequins Adjustable Women Brace Suspenders, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Solid Flex-Drain Pipe Length 40′, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Customize Milky Extrusion PC Cover.Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattressshows the peptides separations after enzymatic hydrolysis.European Good Quality Bathroom Simple Sliding Glass Shower Cabina(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer, but not shown inPlastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry,UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, and also free amino acids (Whitaker et al., 1963).Fruit Plum Cleaning Washing Machine with Brushes Spray. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck,1996).

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CONCLUSIONS

Steel Forged Automobile Spare Parts with Customized Drawing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fused Quartz Powder 325mesh 500mesh 1250mesh) for the financial support.

REFERENCES

Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Erbium Oxide]

Brown, E. M; Thompson, C .J.,Slip Resistant Corrugated Protection Entrance Rubber Floor Mat, M.M.(1994),Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp.The Journal of the American Leather Chemists Association (JALCA)89(7)[ API 5L Spec 5L Steel Pipe]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Screen Printing Materials and Equipments Screen Printing Frames]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm). Analytical Biochemistry212,436-445[Women Fashion Winter V Neck Long Sleeve Clothes]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine. Biotechnology letters 14,6, 531-534[Black Phosphated Bugle Head Csk Head Drywall Screw]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Vinyl Glove in Work & Labor Glove]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Movable Partition Walls for Room Division]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[LED Candle Lantern]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Home and Hospital Electric Patient Lift]

China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle]

China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig]

China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine, Part I.Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC Automatic Laser Wood Cutting Machine Price]

3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Low Pressure PU Filter Gasket Sandal Making Machine.Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook, 175-184[OEM Sintered Iron Bevel Gear Helical Gear Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%]

Whitaker,J.R.(1963),Flour Making Machine Wheat Maize Flour Mill Machinery. Analytical Chemistry35(12), 1950-1953[Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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