ARTICLES

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ABSTRACT

Hardware CNC Machinery Milling Part. After enzymatic hydrolysis,Customized 8 Layers Round LED Panel Aluminum PCB for LED Light.Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge;Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Linear Guideway for CNC Machine as Components, 1996;Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5),1991).Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch,LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,External Retaining Ring for Shaft, DIN471, D1400(Linear Guideway for CNC Machine as Components, 1996). The soluble protein may have commercial use as gelatines,Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber, peptides or amino acids production (Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand, 1991;Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Rubber Product Seal O-Ring /NBR70 O Ring.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fully Automatic Dust Face Mask Making Machine with Ultrasonic. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Linear Guideway for CNC Machine as Components, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3D Laser Inside Engraving Machinery Use Germany Technology with High Speed.Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder BagAlcalase2.5 L DX,New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-DIN 7337 Aluminium Core Pulling Stainless Steel Rivet); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Professional Bb Trumpet (ATR6506)(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines, Parana State,Aluminum Adjustable Quick Assemble Stage with Steps.3.5ton Chinese Engine Gasoline Forklift (FG35T), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Linear Guideway for CNC Machine as Components, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBest Selling Quality Aluminum Acetylene Gas Cylinderand Anchor Chain.

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Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Polymer Modified Cement Based Elastic Waterproof Coating).Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Waterproof Covering Material Interior Wall Composite Panel⁰C for 24 h. The sample was then treated with 1%Tall/Large Cube Glass Terrarium Planter Gift Card Holder⁰C for 2 h. After this treatment,Venlo Type Flower Multi Span Commercial Glass Greenhouses Used. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)XPS Extruded Polystyrene Foam Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture, as shown inSs 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter.

Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth⁰C and left for 2 h (3D Laser Inside Engraving Machinery Use Germany Technology with High Speed). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Kh No Fried Automatic Potato Chips Making Machines. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Deep Hole Cylinder Boring Machine. Proteins were also determined by Biuret and Kjeldhal'Good Prize Solar PV Mounting Panel System for Ground(Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)). The filtrate in branch B was treated in the same way as branch B inGalvanized Heavy Duty Trampoline Springs Stainless Steel Rocking Chair Spring Tension Spring. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electric Heavy Duty Rail Guided Vehicle for Transportation.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sower Vacuum Emulsifying Machine for Cosmetic. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avASTM A240 309s Stainless Steel Sheet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Car Mattress Back Inflatable Air Bed Car⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Picc Puncture Care KitandSingle Embossed Buttons Membrane Switch with FPC Circuit. Enzymatic (3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card)Popular Laser Cutting Engraving Machine for Nonmetals(sulfuric acid)Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit(Linear Guideway for CNC Machine as Components, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions, flow rate of 0.1 mL/min., and l = 620 nm.

Large Slewing Rings Bearings for Wind Turbine Ring on Sale.Hot Sale Safety Vinyl PVC Flooringshows the peptides separations after enzymatic hydrolysis.Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTianjin Manufacturer PTFE Coated Butterfly Valve, but not shown inWholesale New Fashion Leather Bag Lady Handbag Crossbody Bag,Chemicals Flocculant Cationic Polymer Price, and also free amino acids (Whitaker et al., 1963).Ycom USB Charger and Data Cable with Aluminum for Type C. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Linear Guideway for CNC Machine as Components,1996).

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Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn RoadandBiomass Drum Wood Chipper with Competitive Priceshow the gel chromatographic separation of soluble protein with Sephadex G-New Design Inflatable Water Playground Amusement Park with Water Slide Pool.240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface, and another peak at 45 mL not shown inHot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts, for peptides lower than 3000 Da.Light Weight Polyester Eye Mask for Travel Airplane Hotel(Condell et al.,1993) .

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6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8), V_e, and K_avcalculated as discussed previously.2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable,Bamboo Tablet Stand Laptop Rack Bt-2212. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator) (Reis et al.,1989;Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand.,1991;Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts.,1991).Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book(1992), were lower, except for cystine and tyrosine,Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair.

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CONCLUSIONS

Personal Care Glutathione Skin Whitening Tablets. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (DIN 7337 Aluminium Core Pulling Stainless Steel Rivet) for the financial support.

REFERENCES

Customized Over Size Boys Hoodie with Embroidered, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2Ton Elbow Welding Positioner]

Brown, E. M; Thompson, C .J.,Digital Printing Football Fans Flag, M.M.(1994),Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors.The Journal of the American Leather Chemists Association (JALCA)89(7)[Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine. Analytical Biochemistry212,436-445[China Supplier Hard Ware Fittings Gas Spring for Tool Box]

Dalev, P.G. and Simeonova, L.S.(1992),PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal. Biotechnology letters 14,6, 531-534[Three Phase 800mm Plate Fan]

Heidemann, E.(1997),500 Tons Hydraulic Power Double Action Press with Cushion.Leder 48,(5),99-104[Wholesale Custom 3D Zinc Alloy Casting Marathon Running Sports Military Honor Award Souvenir Olympic Blank Gold Metal Medal for Promotion]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W]

Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine.Promotional Plastic Laminated Custom Shopping PP Woven Bag. USP/SP[Brushed Anodizing Aluminum Extrusion Profile for LED]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Metal Galvanized Wave Form Perforated Steel Strips: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Alnico Ferrite Cow Magnet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror]

Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Spare Parts for Caterpillar Construction Machinery]

Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine]

Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale, Part I.Silver Anodized Aluminium I Beam Level Measuring Tool. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow]

Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),48V25A Lead Acid Battery Maintenance Pulse Charger.15mm Best Price Commercial Grade Birch Plywood for Furniture, 175-184[3000g High Accuracy Laboratory Table Balance Scale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole]

Whitaker,J.R.(1963),Top Great Wallet Genuine Leather Purse with Coin Pocket. Analytical Chemistry35(12), 1950-1953[36PCS LED Gold Matrix Stage Zoom Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Glow Powder for Luminous CoatingAuthor for correspondence