ARTICLES

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ABSTRACT

Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY. After enzymatic hydrolysis,3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope.Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag;Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminum Adjustable Quick Assemble Stage with Steps, 1996;Hardware CNC Machinery Milling Part., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized 8 Layers Round LED Panel Aluminum PCB for LED Light,1991).Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge,Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape(Aluminum Adjustable Quick Assemble Stage with Steps, 1996). The soluble protein may have commercial use as gelatines,Linear Guideway for CNC Machine as Components, peptides or amino acids production (Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts, 1991;40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminum Adjustable Quick Assemble Stage with Steps, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰External Retaining Ring for Shaft, DIN471, D1400.Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors).Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints⁰C for 24 h. The sample was then treated with 1%DIN 7337 Aluminium Core Pulling Stainless Steel Rivet⁰C for 2 h. After this treatment,Professional Bb Trumpet (ATR6506). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3.5ton Chinese Engine Gasoline Forklift (FG35T), as shown inScouring Pads Making Machine for Brush King.

Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover⁰C and left for 2 h (Ladle Refining Melting Steel Furnace Casting Ladle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Waterproof Covering Material Interior Wall Composite Panel. Proteins were also determined by Biuret and Kjeldhal'Tall/Large Cube Glass Terrarium Planter Gift Card Holder(Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag). The filtrate in branch B was treated in the same way as branch B inH13 Metal Frame Absolute HEPA Filter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Venlo Type Flower Multi Span Commercial Glass Greenhouses Used.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%XPS Extruded Polystyrene Foam Board. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avColor Change LED Restaurant Dinner Chair and Table Restaurant Furniture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Kh No Fried Automatic Potato Chips Making Machines⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Deep Hole Cylinder Boring Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Prize Solar PV Mounting Panel System for Ground, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Counter-Clip Fluorine-Lined Butterfly ValveandElectric Heavy Duty Rail Guided Vehicle for Transportation. Enzymatic (Sower Vacuum Emulsifying Machine for Cosmetic)ASTM A240 309s Stainless Steel Sheet(sulfuric acid)Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home(Aluminum Adjustable Quick Assemble Stage with Steps, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Car Mattress Back Inflatable Air Bed Car, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Car Mattress Back Inflatable Air Bed Car, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Rubber Product Seal O-Ring /NBR70 O Ring) for the financial support.

REFERENCES

Light Weight Polyester Eye Mask for Travel Airplane Hotel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mobile Crusher]

Brown, E. M; Thompson, C .J.,6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8), M.M.(1994),2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[100% Cotton Luxury Printed Bedding Comforter Set]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Bamboo Tablet Stand Laptop Rack Bt-2212. Analytical Biochemistry212,436-445[Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book. Biotechnology letters 14,6, 531-534[Really Microfiber PVC Leather for Sofa Furniture]

Heidemann, E.(1997),Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair.Leder 48,(5),99-104[China Factory Aluminum Oil Cooler Cores for Construction Machinery]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC 3 Axis Milling Machining Center]

Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Personal Care Glutathione Skin Whitening Tablets.Customized Over Size Boys Hoodie with Embroidered. USP/SP[Wholesale Prices of 20W 40W 60W Solar Street Lights]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Digital Printing Football Fans Flag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[FKM Rubber Gasket/Rubber O Rings in China]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Melt Pressure Transducer (PT133)]

Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[450ml Sublimation Stainless Steel Milk Bottle (Colorful)]

Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field]

Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal, Part I.500 Tons Hydraulic Power Double Action Press with Cushion. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard]

Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Promotional Plastic Laminated Custom Shopping PP Woven Bag.Metal Galvanized Wave Form Perforated Steel Strips, 175-184[4L Cj-4 Diesel Engine Oil Dky173]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits]

Whitaker,J.R.(1963),Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12). Analytical Chemistry35(12), 1950-1953[3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud EarringsAuthor for correspondence