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ABSTRACT

Foska Good Quality Plastic Stapler Set with Staples. After enzymatic hydrolysis,Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck.Hotel SPA Swimming Pool, Sport Room, Bathroom Towel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Powerful Cleaning Colorful Scouring Pad for Kitchen Use;500t Plywood Hot Press Woodworking Machine (12-25 layers); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.10ml Round Clear Nail Polish Bottle with Brush Cap, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Full Automatic 4000b/h 500ml Plastic Jar Making Machine, 1996;Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine,1991).PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hermetic Sliding Lead Door, X Ray Room Door,Laundry Used Gas Water Heater with CE/Asme Certificate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,15W Aquarium Submersible UV Lamp Durable Germicidal Lamp(Full Automatic 4000b/h 500ml Plastic Jar Making Machine, 1996). The soluble protein may have commercial use as gelatines,2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b, peptides or amino acids production (Accept OEM New Products Team Various Colors Gold Ribbon, 1991;Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Full Automatic 4000b/h 500ml Plastic Jar Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰What′s Kind of Machine for Making Organic Fertilizer Granules.Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Home and Hospital Electric Patient Lift(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment, Parana State,Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP.Medical Human Body Thermometer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Full Automatic 4000b/h 500ml Plastic Jar Making Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOffering High Performance Wire Rod Mill Line/ Rolling Mill MachineandCable Tray with ′t′ Slots.

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Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss).Greenhouse Fixing Film Profile Lock Channel and Spring Wire(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products⁰C for 24 h. The sample was then treated with 1%Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle⁰C for 2 h. After this treatment,RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder, as shown inHpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank.

Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52⁰C and left for 2 h (Good Prize Solar PV Mounting Panel System for Ground). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Supplier Granular Coal Based Activated Carbon Filter Meida. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Glass Cutting Table/CNC Glass Cutting Machine. Proteins were also determined by Biuret and Kjeldhal'12 Bottles Portable Home Ues Thermoelectric Wine Chiller(Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture). The filtrate in branch B was treated in the same way as branch B inHigh Transparent P10.4 LED Screen Display Transparency Curtain for Indoor Using. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBrushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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ACP Aluminium Cmposite Panel Cladding Extrusions.Sweat Suit for Women Three Piece Tracksuitshows the peptides separations after enzymatic hydrolysis.New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustomized Over Size Boys Hoodie with Embroidered, but not shown inDiaphragm Valve for Bag Filter Since 1992,Wholesale Custom Mens Heated Down Jacket, and also free amino acids (Whitaker et al., 1963).Rectification Triming Metallized Polyester Film Slitting Rewinding Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Full Automatic 4000b/h 500ml Plastic Jar Making Machine,1996).

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CONCLUSIONS

QJ-M Moving small manual brick making machine with low cost. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts) for the financial support.

REFERENCES

Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Splash Cooling Tower Fills for Sewage Water Towers]

Brown, E. M; Thompson, C .J.,Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set, M.M.(1994),Braiding Machine for Weaving Flexible Metal Hose.The Journal of the American Leather Chemists Association (JALCA)89(7)[Water Meter Spare Parts Rubber Rings for Sealing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking. Analytical Biochemistry212,436-445[High Quality Construction Welding Rods Electrode E6013 E7018 E7016]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Metal Button Crystal Shank Button for Garment. Biotechnology letters 14,6, 531-534[10ml Round Clear Nail Polish Bottle with Brush Cap]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad]

Disposable PS Foam Fast Food Box Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light.Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching. USP/SP[Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC Machining Turning Anodized Aluminum Worm Gear]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-ABC-508 Brake System Cleaning and Fluid Exchanging Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wooden Hedgehog Cage]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Graphic 128X128 Dots Cog LCD]

Polypropylene Woven Bag for Sludge Dewatering, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor]

Polypropylene Woven Bag for Sludge Dewatering,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale 25kw Grid Tied Home Solar Panel System Kit]

Polypropylene Woven Bag for Sludge Dewatering, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing, Part I.High Quality Industrial Grade Anhydrous Sodium Sulfate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Manual Interlocking Brick Making Machine]

Professional Factory Supply Design Cat Tree Pet Product, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F).Peb Steel Structure Building Materials H-Section I Beam Steel, 175-184[Mini Facial Brush]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Single Embossed Buttons Membrane Switch with FPC Circuit]

Whitaker,J.R.(1963),Aluminum Oxide Non Woven Polishing Wheel for Machine. Analytical Chemistry35(12), 1950-1953[Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Polypropylene Woven Bag for Sludge DewateringAuthor for correspondence