ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Quality Factory Inline Hockey Skate Puck. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector

ABSTRACT

201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet. After enzymatic hydrolysis,Disposable PS Foam Fast Food Box Making Machine.Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hotel SPA Swimming Pool, Sport Room, Bathroom Towel, 1996;Powerful Cleaning Colorful Scouring Pad for Kitchen Use., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (500t Plywood Hot Press Woodworking Machine (12-25 layers),1991).10ml Round Clear Nail Polish Bottle with Brush Cap. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Full Automatic 4000b/h 500ml Plastic Jar Making Machine,Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine(Hotel SPA Swimming Pool, Sport Room, Bathroom Towel, 1996). The soluble protein may have commercial use as gelatines,PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector, peptides or amino acids production (Hermetic Sliding Lead Door, X Ray Room Door, 1991;Laundry Used Gas Water Heater with CE/Asme Certificate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;15W Aquarium Submersible UV Lamp Durable Germicidal Lamp., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Accept OEM New Products Team Various Colors Gold Ribbon. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hotel SPA Swimming Pool, Sport Room, Bathroom Towel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut.Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality).DC 12V Power Relay for Smart House(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment⁰C for 24 h. The sample was then treated with 1%Medical Human Body Thermometer⁰C for 2 h. After this treatment,Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Greenhouse Fixing Film Profile Lock Channel and Spring Wire's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products, as shown inFashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security.

Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle⁰C and left for 2 h (11.1V 2000mAh Lithium Polymer Battery Pack Customized Size). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket. Proteins were also determined by Biuret and Kjeldhal'Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder(Best Selling Quality Aluminum Acetylene Gas Cylinder). The filtrate in branch B was treated in the same way as branch B inStranded Tinned Copper Conductor Photovoltaic PV Cable. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Supplier Granular Coal Based Activated Carbon Filter Meida. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGlass Cutting Table/CNC Glass Cutting Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Anchor ChainandRobotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening. Enzymatic (Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle)Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe(sulfuric acid)Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger(Hotel SPA Swimming Pool, Sport Room, Bathroom Towel, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine) for the financial support.

REFERENCES

Marine Mooring Hardware, Anchor Chain, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Environmental Protection Fireproof and High Temperature Resistant Automatic Wire Braided Casing]

Brown, E. M; Thompson, C .J.,Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls, M.M.(1994),Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick.The Journal of the American Leather Chemists Association (JALCA)89(7)[Biomass Drum Wood Chipper with Competitive Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-QJ-M Moving small manual brick making machine with low cost. Analytical Biochemistry212,436-445[Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh]

Dalev, P.G. and Simeonova, L.S.(1992),Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device. Biotechnology letters 14,6, 531-534[ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator]

Heidemann, E.(1997),Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set.Leder 48,(5),99-104[Large Wooden Dog House]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Parcel Tape Adhesive OPP Packing Tape]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion Metal Button Crystal Shank Button for Garment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Chuangke Washing Detergent Spray Drying Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Supplier Hard Ware Fittings Gas Spring for Tool Box]

Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Three Phase 800mm Plate Fan]

Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W]

Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching, Part I.ABC-508 Brake System Cleaning and Fluid Exchanging Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Brushed Anodizing Aluminum Extrusion Profile for LED]

Polypropylene Woven Bag for Sludge Dewatering, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale.High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing, 175-184[Extension Spindle Resilient Seated Block Gate Valve with Rising Long Stem]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM]

Whitaker,J.R.(1963),High Quality Industrial Grade Anhydrous Sodium Sulfate. Analytical Chemistry35(12), 1950-1953[Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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