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ABSTRACT

Stainless Steel Screens / Wedge Wire Filter Element. After enzymatic hydrolysis,Amazon Armor TPU Silicone Phone Case for Honor 10 Lite.EPS Foam Sheet Extrusion Machine for Fast Food Box Making. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe;Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel, 1996;YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Stainless Steel Plate Flange,1991).High Quality Low Price Top Selling Fashion Summer Boy Sandals. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Easy Operational Cup Cake Filling Machine,Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Folding Play Mat Crawl Play Mat(Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel, 1996). The soluble protein may have commercial use as gelatines,Wooden Grain Perforated Panel for Metal Mesh Curtain Wall, peptides or amino acids production (Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1, 1991;Folding  Platform Hand  Trolley , 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pump Flower Sprinkler with Automatic Watering System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Low Carbon Heavy Galvanized Steel Wire.Long Distance Measurement Power Laser Digital Meter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality CO2 Laser Marking Machine with Ce, Parana State,3D CNC Router for Wood Cutting Machine.New Design Inclined Plane 18W LED Wall Washer Lighting, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable DeviceandEPS Foam Sheet Extrusion Machine for Fast Food Box Making.

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2ton R Type Electric Wire Rope Hoist with Single Speed(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S)).Stainless Steel Body Infrared Burner Good Selling Gas Stove(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier⁰C for 24 h. The sample was then treated with 1%Adhesive CAS 9003-35-4 Phenolic Resin⁰C for 2 h. After this treatment,Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Manufacture Best Choice Liquid Flocculant for Ink Removal's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Dipped Galvanized Oval Wire Brazil, as shown inAutomatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08).

Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys⁰C and left for 2 h (Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit. Proteins were also determined by Biuret and Kjeldhal'Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage, Drink Silicone PVC Coaster Beer Drink Coaster Cup Coaster (YB-CC-1)(Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence). The filtrate in branch B was treated in the same way as branch B inPaint Brush Fabric Industrial_Knitting_Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Foil PE Bottle Cap Induction Seal Liner.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSophisticated Durable Membrane Panel/Panel with 3m Glue(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.HDPE Polyethylene PE Pipe for Water Supply⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Portable Mini Fridge with Light Box, V_e, and K_avcalculated as discussed previously.Agriculture Film for Corn Silage Wrap Baler,Cheap Gabion Wire Mesh Box. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery) (Reis et al.,1989;Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1.,1991;YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine.,1991).Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film(1992), were lower, except for cystine and tyrosine,Stainless Steel Double Layer Filter USD in POY Machines.

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CONCLUSIONS

8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key) for the financial support.

REFERENCES

Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Colored Polycarbonate Twin Wall Sheet]

Brown, E. M; Thompson, C .J.,Graphite Ring Good Lubrication, M.M.(1994),China Tower 6-Axis Screwdriver Machine Robot Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Folding Round Picnic Caravan Table RV Picnic Table Camping Table]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Walking Long Distance Surveying Instrument Meter Measuring Wheel]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mslqs-P Needle-Free Injector/Needleless Syringe Price. Analytical Biochemistry212,436-445[Micro DC 24V Worm Gear Brushless Motor for Medical Equipment]

Dalev, P.G. and Simeonova, L.S.(1992),YAMAHA Yv100II Nozzle Horder Square Head Short Type. Biotechnology letters 14,6, 531-534[Carboxymethyl Cellulose CAS No. 9004-32-4]

Heidemann, E.(1997),Juice Doypack Packing Machine (ZLDB-4).Leder 48,(5),99-104[Commercial Jungle Indoor Playground]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Funny Newest Design for Children Theme Outdoor Playground Park: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Table Hard Ice Cream Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover]

High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service]

High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Air Cooled Split Type Ducted Air Conditioner AC Unit]

High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM, Part I.Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Full Automatic 4000b/h 500ml Plastic Jar Making Machine]

Good Strength GRP Cooling Towers for Mechanical Cooling, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash.Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief, 175-184[Fastener Water Heater Expansion Anchor Bolt with L Shape Hook Yellow Galvanized]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Made in China Lqa Strap Banding Tool]

Whitaker,J.R.(1963),Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts. Analytical Chemistry35(12), 1950-1953[Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Modern Pharmacy Vacuum Filter PressAuthor for correspondence