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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.UF Membrane Aqu160 with Competitive Price and High Quality, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Atf-805 Auto Car Transmission System Flushing Machine, 1996;43 Glass Beads Cat Eye Aluminum Road Stud., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041),1991).High Precision C45 Steel CNC Lathe Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max,CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1(Atf-805 Auto Car Transmission System Flushing Machine, 1996). The soluble protein may have commercial use as gelatines,Food Grade PTFE Coated Fiberglass Mesh Baking Sheet, peptides or amino acids production (Beverage Cooling Cube Ice Making/Ice Maker Machinery, 1991;Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Transparent Composite Fiberglass Roof Tiles., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Two Layer License Plates with Wide Border Embossed. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Atf-805 Auto Car Transmission System Flushing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electrical Barber Shop Equipment Electric Hair Curling Wand Iron.Really Microfiber PVC Leather for Sofa Furniture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:94V0 Circuit Board Mobile Phone PCB Motherboard(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing).Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness⁰C for 24 h. The sample was then treated with 1%LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light⁰C for 2 h. After this treatment,Safest Baby Healthcare and Grooming Kit for Baby Gift. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro, as shown inCustom Aluminum Cover Frame Half Aluminum Cover Profile.

Micro DC 24V Worm Gear Brushless Motor for Medical Equipment⁰C and left for 2 h (Low Fuel Consumption Diesel Generator Economic Diesel Power 80kw 100kVA). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1.6 Ton Hand Lifting Tool Lever Chain Block. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CNC Machining Service High Precision CNC Mechanical Parts. Proteins were also determined by Biuret and Kjeldhal'Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks(Carbon Graphite Crucible). The filtrate in branch B was treated in the same way as branch B inNenz Lithium (Li-ion) Cordless Combo Rotary Hammer for Decoration (NZ80). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Printed 100% Polyester Tcheap Wholesale Blankets. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFood Grade Material 304/316 Double Diaphragm Pumps(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Power From Han′s GS 2000W Laser Cutter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Adhesive Powder for Skim Coat/Wall Putty HPMC) for the financial support.

REFERENCES

6 Inch Plastic Breathing Cap for The IBC Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable]

Brown, E. M; Thompson, C .J.,Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability, M.M.(1994),Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging.The Journal of the American Leather Chemists Association (JALCA)89(7)[Metal Galvanized Wave Form Perforated Steel Strips]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Price PVC Coated Fiberglass Mesh Insect Window Screen]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree. Analytical Biochemistry212,436-445[43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump]

Dalev, P.G. and Simeonova, L.S.(1992),OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part. Biotechnology letters 14,6, 531-534[ 3FT 3000lm IP66 Approved]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Swim Shorts Grey Mens Board Shorts with Mesh Liner]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Freshwater Pearl Butterfly Flower Bride Hairpin: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Transparent Composite Fiberglass Roof Tiles]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.qingdaoflrope.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine]

Air Bubble Insulation Thermal Building Insulation Material, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ with Handle Vegetable Oil Nonstick]

Air Bubble Insulation Thermal Building Insulation Material,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Printed 100% Polyester Tcheap Wholesale Blankets]

Air Bubble Insulation Thermal Building Insulation Material, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rechargeable Battery Operated Cooling Folding Electric Fan, Part I.High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[150kg Per Hour Frozen French Fries Production Line]

21.5′′ Wall Mount Display Capacitive Touch Monitor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pagoda Gazebo Tent in Guangzhou for Sale.Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe, 175-184[Gas Spring]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket]

Whitaker,J.R.(1963),Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine. Analytical Chemistry35(12), 1950-1953[Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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