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ABSTRACT

3HP Industrial Cooling System Water Cooled Chiller. After enzymatic hydrolysis,OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress.Wood Veneer Peeling and Clipping Combined Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Si3n4 Full Ceramic  Bearing  608 Size 8X22X7, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall, 1996;Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Corrugated Board Production Line,1991).Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Car Air Conditional Compressor Magetic Clutch 5pk,High Quality Engieered Veneer Faced Commericial Plywood,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hex Thin Head Nut(32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall, 1996). The soluble protein may have commercial use as gelatines,Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2, peptides or amino acids production (Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap, 1991;Spare Parts for Caterpillar Construction Machinery, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.50 Grams Ozone Producing Device for Makeup Toner Producing Plant.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Flat Pack Temperary Container House 20FT 40FT. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sweat Suit for Women Three Piece Tracksuit.Cold Rolled Stainless Steel Strip /Coils 410/430/409-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:FRP Water Tank GRP Fishing Tanks From Factory Supply(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Blue Steel Packing Strip(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale 25kw Grid Tied Home Solar Panel System Kit).High Quality Powder and Wax Brush with Wooden Handle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cheap Price Paper Straws Individually Wrapped Drinking Straw⁰C for 24 h. The sample was then treated with 1%ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality⁰C for 2 h. After this treatment,Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Smelting and Heating Induction Machine (GW-HY187), as shown inManual Interlocking Brick Making Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sell Mechanical Bar Screen for Water Treatment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.700 mm RP HP UHP Graphite Electrode Sold at Factory Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Union Metal Steel Building and Construction Material, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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800W Electric Motor Price Lawn Mower Motor Concrete Mixer.Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machineshows the peptides separations after enzymatic hydrolysis.Air Cooled Split Type Ducted Air Conditioner AC Unit(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFolding  Platform Hand  Trolley , but not shown inSophisticated Durable Membrane Panel/Panel with 3m Glue,Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric, and also free amino acids (Whitaker et al., 1963).High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall,1996).

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CONCLUSIONS

Various Types of Plastic Hooks Mould From Manufacturers. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder) for the financial support.

REFERENCES

New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Customized Casting Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower, M.M.(1994),Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift.The Journal of the American Leather Chemists Association (JALCA)89(7)[Lower Noise Colled Rolled Ball Screw]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Galvanized Eye Grab Bend Hook]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India. Analytical Biochemistry212,436-445[Chinese Galvanized Rectangular Tube for Buildings Materials]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PVC Connector Sleeve Soft Plastic Battery Terminal Covers]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-E171 Standrad Titanium Dioxide Food Grade A200: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Supplier Steel Machine Bellows Cover Telescopic Shield Cover]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Best Quality 12V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla]

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Elbow Forming Machine Gorelocker HVAC Duct Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Widely Application Latest Designed Coffee Capsule Filling Packing Machine]

Elbow Forming Machine Gorelocker HVAC Duct Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses, Part I.L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem]

Whitaker,J.R.(1963),Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter. Analytical Chemistry35(12), 1950-1953[Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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