ARTICLES

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ABSTRACT

Home Machine 2019 New Commercial Treadmill Gym Equipment. After enzymatic hydrolysis,Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card.Residential Reverse Osmosis 0.0001um Water Filter Water Filtration. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Folding Round Picnic Caravan Table RV Picnic Table Camping Table, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits, 1996;3HP Industrial Cooling System Water Cooled Chiller., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress,1991).Wood Veneer Peeling and Clipping Combined Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone,Wholesale High Quality Decoration Oil Painting, Home Decoration Painting, Art Painting (abstract sailing oil painting),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Si3n4 Full Ceramic  Bearing  608 Size 8X22X7(Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits, 1996). The soluble protein may have commercial use as gelatines,32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall, peptides or amino acids production (Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker, 1991;Corrugated Board Production Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Car Air Conditional Compressor Magetic Clutch 5pk.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Engieered Veneer Faced Commericial Plywood. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hex Thin Head Nut.Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Sweat Suit for Women Three Piece Tracksuit, Parana State,Water Cooled Low Temperature Screw Chiller for Industrial Workshop.Cold Rolled Stainless Steel Strip /Coils 410/430/409, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGalvanized Solar System Mounting Bracket ConnectorandChina Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes.

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FRP Water Tank GRP Fishing Tanks From Factory Supply(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine).Decorative House Stone Coated Metal Roof Tile(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig⁰C for 24 h. The sample was then treated with 1%6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder⁰C for 2 h. After this treatment,Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use, as shown inMining Machinery Sand Vibrating Sieve for River Sand.

Blue Steel Packing Strip⁰C and left for 2 h (Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Powder and Wax Brush with Wooden Handle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cheap Price Paper Straws Individually Wrapped Drinking Straw. Proteins were also determined by Biuret and Kjeldhal'ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality(S235jo S235jr Steel Pipe Tube Shs Rhs). The filtrate in branch B was treated in the same way as branch B inwww.miracle-leather-bag.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSmelting and Heating Induction Machine (GW-HY187)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Home Products 16′ Perforated Flex Pipe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1220 Fork Length 3-Wheel Electric Forklift Truck, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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800W Electric Motor Price Lawn Mower Motor Concrete Mixer, V_e, and K_avcalculated as discussed previously.Air Cooled Split Type Ducted Air Conditioner AC Unit,Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Gym Fitness Equipment Adjustable Weight Bench) (Reis et al.,1989;Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker.,1991;3HP Industrial Cooling System Water Cooled Chiller.,1991).High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet(1992), were lower, except for cystine and tyrosine,Gym Fitness Equipment Adjustable Weight Bench.

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CONCLUSIONS

Factory Sell P7.8 Transparent LED Display Screen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (50 Grams Ozone Producing Device for Makeup Toner Producing Plant) for the financial support.

REFERENCES

Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Half Finger Bicycle Gloves]

Brown, E. M; Thompson, C .J.,Original Weichai Engine Truck Spare Parts Valve Cap 61500050105, M.M.(1994),Sliding Panel Saw Heavy Duty CNC Band Saw for Furniture Ban Resaw Machine for Lumber, Round Log Slinding Sawmill, Band Resaw for Lumber.The Journal of the American Leather Chemists Association (JALCA)89(7)[Reusable Metal Stainless Steel Drinking Straws]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim. Analytical Biochemistry212,436-445[ D1400]

Dalev, P.G. and Simeonova, L.S.(1992),Charms for Jewelry Making (Sold Per Bag 10PCS Inside). Biotechnology letters 14,6, 531-534[Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)]

Heidemann, E.(1997),Fused Quartz Powder 325mesh 500mesh 1250mesh.Leder 48,(5),99-104[Gbr-PT6000-7000 Sky Tracker Light Outdoor Serach Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Pressure Fitting Quick Coupling Hydraulic Plug]

22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Various Types of Plastic Hooks Mould From Manufacturers.New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle. USP/SP[Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Hv / Coating Material Grade CMC Medium Viscosity]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Efficiency Fine Powder Vibrating Screener]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Cnc Auto Car Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules]

Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry]

Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank]

Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Soft Baby Suites Dinosaure Pijamas Kigurumi, Part I.Ghrp-2 Building Muscle Pharmaceutical Peptide 5mg/Vial 99.80% High Purity From Lab Supply. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Double Roller Rotary Wood Peeling Machine with CE for Sale]

High Density Celuca Kitchen Cabinets PVC Foam Board, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc.E171 Standrad Titanium Dioxide Food Grade A200, 175-184[Deep Bowl Kitchen Sink]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter]

Whitaker,J.R.(1963),Elbow Forming Machine Gorelocker HVAC Duct Making Machine. Analytical Chemistry35(12), 1950-1953[Factory Price High Carbon Isostatic Graphite Electrodes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball ValveAuthor for correspondence