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ABSTRACT

Flat Pack Temperary Container House 20FT 40FT. After enzymatic hydrolysis,Sweat Suit for Women Three Piece Tracksuit.Cold Rolled Stainless Steel Strip /Coils 410/430/409. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;FRP Water Tank GRP Fishing Tanks From Factory Supply;Decorative House Stone Coated Metal Roof Tile; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder, 1996;Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine,1991).PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Blue Steel Packing Strip,High Quality Powder and Wax Brush with Wooden Handle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cheap Price Paper Straws Individually Wrapped Drinking Straw(6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder, 1996). The soluble protein may have commercial use as gelatines,ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality, peptides or amino acids production (Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF, 1991;Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Smelting and Heating Induction Machine (GW-HY187)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Home Products 16′ Perforated Flex Pipe. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1220 Fork Length 3-Wheel Electric Forklift Truck.Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:FM USB Radio Audio Power Amplifier for Home Bar Club(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck, Parana State,50 Grams Ozone Producing Device for Makeup Toner Producing Plant.Union Metal Steel Building and Construction Material, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2and100% Cotton Luxury Printed Bedding Comforter Set.

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Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Really Microfiber PVC Leather for Sofa Furniture).450ml Sublimation Stainless Steel Milk Bottle (Colorful)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Lower Noise Colled Rolled Ball Screw⁰C for 24 h. The sample was then treated with 1%Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls⁰C for 2 h. After this treatment,3.0kw Spindle CNC Router with Certificate for Woodworking. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)800W Electric Motor Price Lawn Mower Motor Concrete Mixer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Air Cooled Split Type Ducted Air Conditioner AC Unit, as shown inCNC 3 Axis Milling Machining Center.

Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric⁰C and left for 2 h (Wholesale Prices of 20W 40W 60W Solar Street Lights). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Gym Fitness Equipment Adjustable Weight Bench. Proteins were also determined by Biuret and Kjeldhal'Factory Sell P7.8 Transparent LED Display Screen(Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine). The filtrate in branch B was treated in the same way as branch B inShantui Official Manufacturer Hzs25se mobile Concrete Mixing Plant. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Original Weichai Engine Truck Spare Parts Valve Cap 61500050105. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSliding Panel Saw Heavy Duty CNC Band Saw for Furniture Ban Resaw Machine for Lumber, Round Log Slinding Sawmill, Band Resaw for Lumber(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Charms for Jewelry Making (Sold Per Bag 10PCS Inside)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fused Quartz Powder 325mesh 500mesh 1250mesh, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Various Types of Plastic Hooks Mould From Manufacturers, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire ConnectorandNew Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle. Enzymatic (10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower)Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift(sulfuric acid)Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India(6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Soft Baby Suites Dinosaure Pijamas Kigurumi, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Soft Baby Suites Dinosaure Pijamas Kigurumi, flow rate of 0.1 mL/min., and l = 620 nm.

Ghrp-2 Building Muscle Pharmaceutical Peptide 5mg/Vial 99.80% High Purity From Lab Supply.Melt Pressure Transducer (PT133)shows the peptides separations after enzymatic hydrolysis.High Density Celuca Kitchen Cabinets PVC Foam Board(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in450ml Sublimation Stainless Steel Milk Bottle (Colorful), but not shown inSecurity RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field,2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc, and also free amino acids (Whitaker et al., 1963).E171 Standrad Titanium Dioxide Food Grade A200. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder,1996).

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CONCLUSIONS

Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Glow Powder for Luminous Coating) for the financial support.

REFERENCES

New Fashion PVC Sequins Adjustable Women Brace Suspenders, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dimmable Study Lamp]

Brown, E. M; Thompson, C .J.,Solid Flex-Drain Pipe Length 40′, M.M.(1994),ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator.The Journal of the American Leather Chemists Association (JALCA)89(7)[4X4 Galvanized Welded Wire Mesh Panel (FACTORY)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sinicline 2018 Hot Sell Custom Printing Thank You Cards]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case. Analytical Biochemistry212,436-445[Metal Material and Sports Theme Custom Metal Medal]

Dalev, P.G. and Simeonova, L.S.(1992),Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500. Biotechnology letters 14,6, 531-534[34*25cm Office File Pocket Felt A4 Document Bag]

Heidemann, E.(1997),W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/.Leder 48,(5),99-104[Tactical Airsoft Combat Uniform Emerson Frog Suit Cp Camo]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8)]

Spare Parts for Caterpillar Construction Machinery, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cake/Cookies/Bread Automatic Package and Packing Machine.Customize Milky Extrusion PC Cover. USP/SP[Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ 800 Wog]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-European Good Quality Bathroom Simple Sliding Glass Shower Cabina: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Spare Parts Custom Injection Plastic Moulding Manufacturer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Full Color PVC Celuka Crust Rigid Foam Board Supplier From China]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality]

UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fruit Plum Cleaning Washing Machine with Brushes Spray-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Food Grade PTFE Coated Fiberglass Mesh Baking Sheet]

UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Road Safety Warning Barricade LED Solar Powered Blinking Light]

UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Manufacture Electromagnetic Impulse Solenoid Valve, Pulse Operated Solenoid Valve for Dust Collector, Part I.11.1V 2000mAh Lithium Polymer Battery Pack Customized Size. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Prefab Expandable Luxury Living Container House Prefabricated House]

Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Jh21 Series Pneumatic Power Press CNC Punching Machine.Coal Fried Steam Boiler for Industry with Double Drum, 175-184[High Quality 29424 Spherical Roller Thrust Bearings]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck]

Whitaker,J.R.(1963),Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers. Analytical Chemistry35(12), 1950-1953[Deep Hole Cylinder Boring Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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