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ABSTRACT

Disposable Sterile Twist Top Blood Lancet. After enzymatic hydrolysis,Industrial Control Housing with Plate Shape (XD-CC04).Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Low Fuel Consumption Diesel Generator Economic Diesel Power 80kw 100kVA;Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC 3 Axis Milling Machining Center, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting, 1996;Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (4 Axis CNC Woodworking Machinery Engraver Router,1991).Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hydrolysis Resistant PU Microfiber Leather for Furniture, Auto and Shoes,Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pre-Seasoned Cast Iron Baking Stone Cookware(Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting, 1996). The soluble protein may have commercial use as gelatines,Compression Polyester Light Ankle Support Brace for Youth, peptides or amino acids production (Good Quality White PVC Rigid Foam Board for Iran Market, 1991;Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Precision Piston Ring Good Shape Ability High Quality Surface.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Farm Relief Plastic Automatic Air Release Valve.60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Multi Color Spade Made of Steel and Panting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Compression Polyester Light Ankle Support Brace for Youth).Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine⁰C for 24 h. The sample was then treated with 1%New Condition Hot Sale Type Air Cooled Ice Cube Maker⁰C for 2 h. After this treatment,Stainless Steel Hinge Pin, Locking Hinge Pin. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)4 Way Stretch Running Gym Shorts Unique Women Short Pants's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)VHDCI Connector, 100p, Solder 0.8mm Pitch, as shown in 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube.

6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube⁰C and left for 2 h (Road Construction Fabric Non-Woven Geotextile 200GSM). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Must 4kw 2HP Pump Power Inverter Ags Bts Available. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask. Proteins were also determined by Biuret and Kjeldhal'Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling(Geyi Disposable Laparoscopic Visible Optical Trocar). The filtrate in branch B was treated in the same way as branch B inCo-Extrusion Board Machine Wholesaler Quotes/PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%100W 120W 150W Outdoor Solar LED Street Lighting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Economic Full Automated SMT Production Line PCB Production Machinery⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Advanced Unlocking Tool for LocksmithandBank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206). Enzymatic (Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600)HDPE Geomembrane Lining Price for Pond Liner Film(sulfuric acid)China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW(Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Varied Colored As568 Food Grade Soft Rubber Silicone O Ring, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Varied Colored As568 Food Grade Soft Rubber Silicone O Ring, flow rate of 0.1 mL/min., and l = 620 nm.

2.0mm Yellow Markers for Fiber Optic Multi Patch Cord.Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in Chinashows the peptides separations after enzymatic hydrolysis.Chinese Galvanized Rectangular Tube for Buildings Materials(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTop Great Wallet Genuine Leather Purse with Coin Pocket, but not shown inPCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header,China Customized Chilled Cast Iron Roll, and also free amino acids (Whitaker et al., 1963).IMTEK High Precision and Rigidity Linear Guides for CNC machinery. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting,1996).

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Used/Second Hand 120mpm Continous Galvanizing Line/CgiandCabinet Shelf Support Pin Furniture Hardware Fittingsshow the gel chromatographic separation of soluble protein with Sephadex G-Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask.Yashi Touch Screen Smart Interactive Whiteboard for Office, and another peak at 45 mL not shown inIntegrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment, for peptides lower than 3000 Da.Electric Fireplace PCBA with Double Layers Circuit Board(Condell et al.,1993) .

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China Shipping Logistics to Stockholm, V_e, and K_avcalculated as discussed previously.Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting,Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile) (Reis et al.,1989;Good Quality White PVC Rigid Foam Board for Iran Market.,1991;Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter.,1991).Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#(1992), were lower, except for cystine and tyrosine,Animal Agricultural Damping Horse Stall Rubber Cow Mat.

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CONCLUSIONS

CNC Automatic Laser Wood Cutting Machine Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sw Type BOPP Packaging Adhesive Tape Coating Machine) for the financial support.

REFERENCES

Yc Pure White Nylon Powder for 3D Printing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Carbon Steel Plate Pn16 Pipe Flange]

Brown, E. M; Thompson, C .J.,Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats, M.M.(1994),Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM.The Journal of the American Leather Chemists Association (JALCA)89(7)[1220*2440mm Concrete Formwork black film faced plywood]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ JIS G 3461 ASTM A179 Seamless Boiler Pipe]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Liquid Spouted Stand up Pouch with Custom Logo. Analytical Biochemistry212,436-445[Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug]

Dalev, P.G. and Simeonova, L.S.(1992),Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container. Biotechnology letters 14,6, 531-534[Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening]

Heidemann, E.(1997),Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel.Leder 48,(5),99-104[Water Heat Pump]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container]

Dn32 (11/4) BSPT Thread Air Actuated Piston Valves, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Modern Pharmacy Vacuum Filter Press.100% Cotton Luxury Printed Bedding Comforter Set. USP/SP[Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mslqs-P Needle-Free Injector/Needleless Syringe Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mouse Pad with Gel Wrist Rest: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Natural Hemp Shower Bath Sponge Pad]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Temperature Alternative Stainless Steel Band in Different Package]

Custom Size Christmas Gift Personalized Watch Boxes for Men, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Best Selling Quality Aluminum Acetylene Gas Cylinder-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels]

Custom Size Christmas Gift Personalized Watch Boxes for Men,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC]

Custom Size Christmas Gift Personalized Watch Boxes for Men, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Coal Mine Mini Electric Excavator with Quick Coupling, Part I.2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder]

Food Grade Transparent Cookie Airtight Plastic Container Jars, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory.Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women, 175-184[Chinese Truck Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Heavy Industry Electric Transport Vehicle on Rails]

Whitaker,J.R.(1963),Factory Direct Sale Single Cloth Hanger Hook. Analytical Chemistry35(12), 1950-1953[CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Juice Doypack Packing Machine (ZLDB-4)Author for correspondence