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ABSTRACT

Wholesale 375ml Dark Green Glass Ice Wine Bottles. After enzymatic hydrolysis,Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle.Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cog Graphic FPC LCD Display Module;Power Fitting- Hot DIP Galvanizing Bolt with Nut; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Slewing Gear for Tower Crane Slewing Winch Reducer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine, 1996;7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes,1991).Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8),Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved(Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine, 1996). The soluble protein may have commercial use as gelatines,Single Screw Extruder for Plastic PP/PE Pipe Production Line, peptides or amino acids production (Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck, 1991;Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Gold Star Square Glass Candle Holder for Wedding Decoration., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.11kg 24L Low Pressure LPG Gas Cylinder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Commercial Use Click Lock Standard Spc Vinyl Plank Flooring. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop.High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Precision Customized OEM Auto Hardware Lathe CNC Machining Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 New Portable Mini Dental X-ray Machine).Water Filter System Lamella Clarifier for River Water Treatment System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C)⁰C for 24 h. The sample was then treated with 1%Cabinet Shelf Support Pin Furniture Hardware Fittings⁰C for 2 h. After this treatment,Reasonable Price High Quality Nylon Cable Gland. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Disposable Anesthesia Breathing Circuit Tube, as shown inFull-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line.

High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type⁰C and left for 2 h (Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126. Proteins were also determined by Biuret and Kjeldhal'Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks(Vulcanizing Machine /Rubber Tiles Vulcanzing Press). The filtrate in branch B was treated in the same way as branch B in3D Printer & CNC 8mm Hardened Induction Hard Chrome Steel Bearing Linear Shaft. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Counter-Clip Fluorine-Lined Butterfly Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%DC Brushless Motor for CNC Machine with ISO9001. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Manufacturer Customized Plastic Injection Molding⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12)andTest Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine. Enzymatic (60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light)Solar Energy Storad Power Generation System(sulfuric acid)110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K)(Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Aluminum Cover Frame Half Aluminum Cover Profile, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Aluminum Cover Frame Half Aluminum Cover Profile, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line) for the financial support.

REFERENCES

Lab Equipment Sr Paper Pulp Beating Freeness Test Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tool Suitcase]

Brown, E. M; Thompson, C .J.,CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy, M.M.(1994),Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Bar Sink]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Extruded Alloy Aluminum Tube 1060 1070 3A21]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001). Analytical Biochemistry212,436-445[Hairs Comb for Pet Dog Cats]

Dalev, P.G. and Simeonova, L.S.(1992),Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making. Biotechnology letters 14,6, 531-534[DIN 7337 Aluminium Core Pulling Stainless Steel Rivet]

Heidemann, E.(1997),Double Roller Rotary Wood Peeling Machine with CE for Sale.Leder 48,(5),99-104[Knitted Toque]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Popular Lovely Animal Design Inflatable Slide for Kids]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Avocat Nutrition Sport]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mushroom Head Stud Sert]

Ladle Refining Melting Steel Furnace Casting Ladle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Lishui Factory High Quality Metal Rolling Ball Screw-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light]

Ladle Refining Melting Steel Furnace Casting Ladle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part]

Ladle Refining Melting Steel Furnace Casting Ladle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Y-Spring Threaded Check Valve, 800 Wog, Part I.OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls]

Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Galvanized Eye Grab Bend Hook.Transparent LED Display P3.91 Glass Wall LED Screen, 175-184[Ro Bw Membrane]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Push Pull Small Cable to Cable Connector]

Whitaker,J.R.(1963),Surgical Power Tool Medical Care Orthopedic Mini Bone Saw. Analytical Chemistry35(12), 1950-1953[Elegant Europe Dessign Pillow Top Mattress]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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