ARTICLES

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ABSTRACT

Atf-805 Auto Car Transmission System Flushing Machine. After enzymatic hydrolysis,43 Glass Beads Cat Eye Aluminum Road Stud.Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Precision C45 Steel CNC Lathe Parts;I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1, 1996;Food Grade PTFE Coated Fiberglass Mesh Baking Sheet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Beverage Cooling Cube Ice Making/Ice Maker Machinery,1991).Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Transparent Composite Fiberglass Roof Tiles,Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Two Layer License Plates with Wide Border Embossed(Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1, 1996). The soluble protein may have commercial use as gelatines,Electrical Barber Shop Equipment Electric Hair Curling Wand Iron, peptides or amino acids production (Really Microfiber PVC Leather for Sofa Furniture, 1991;94V0 Circuit Board Mobile Phone PCB Motherboard, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Adhesive Powder for Skim Coat/Wall Putty HPMC. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰4X4 Galvanized Welded Wire Mesh Panel (FACTORY).Cotton Yarn Computerized Lace Knitting Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Caps Making Machine / Injection Molding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Safest Baby Healthcare and Grooming Kit for Baby Gift, Parana State,UF Membrane Aqu160 with Competitive Price and High Quality.Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Sale Beautiful RFID NFC Ntag213 Epoxy CardandReusable Metal Stainless Steel Drinking Straws.

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Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( D1400).Micro DC 24V Worm Gear Brushless Motor for Medical Equipment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1.6 Ton Hand Lifting Tool Lever Chain Block⁰C for 24 h. The sample was then treated with 1%CNC Machining Service High Precision CNC Mechanical Parts⁰C for 2 h. After this treatment,Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Printed 100% Polyester Tcheap Wholesale Blankets, as shown inCrushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002).

Food Grade Material 304/316 Double Diaphragm Pumps⁰C and left for 2 h (High Pressure Fitting Quick Coupling Hydraulic Plug). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator. Proteins were also determined by Biuret and Kjeldhal'High Power From Han′s GS 2000W Laser Cutter(Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor). The filtrate in branch B was treated in the same way as branch B inHigh Quality Thermal Padding Sleeveless Hooded Vest for Men. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Widely Application Latest Designed Coffee Capsule Filling Packing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avZipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Density Rock Wool Wall Board with Thermal Insulation Performance⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hv / Coating Material Grade CMC Medium ViscosityandChina Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery. Enzymatic (China PAC Water Flocculating Agent for Water Treatment)Factory Price High Carbon Isostatic Graphite Electrodes(sulfuric acid)Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets(Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film, flow rate of 0.1 mL/min., and l = 620 nm.

8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light.High Efficiency Fine Powder Vibrating Screenershows the peptides separations after enzymatic hydrolysis.Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSize0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules, but not shown in3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry,Powder/Spices Powder Packing Machine with Four/Three Side Seal, and also free amino acids (Whitaker et al., 1963).Black Phosphated Bugle Head Csk Head Drywall Screw. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1,1996).

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6 Inch Plastic Breathing Cap for The IBC Valve, V_e, and K_avcalculated as discussed previously.Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability,Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (E171 Standrad Titanium Dioxide Food Grade A200) (Reis et al.,1989;Really Microfiber PVC Leather for Sofa Furniture.,1991;Food Grade PTFE Coated Fiberglass Mesh Baking Sheet.,1991).Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree(1992), were lower, except for cystine and tyrosine,OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part.

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CONCLUSIONS

Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness) for the financial support.

REFERENCES

Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[LCD Screen Video Greeting Card with Printed Logo]

Brown, E. M; Thompson, C .J.,Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer, M.M.(1994),Freshwater Pearl Butterfly Flower Bride Hairpin.The Journal of the American Leather Chemists Association (JALCA)89(7)[What′s Kind of Machine for Making Organic Fertilizer Granules]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Air Bubble Insulation Thermal Building Insulation Material. Analytical Biochemistry212,436-445[Evaporator Finned Tube]

Dalev, P.G. and Simeonova, L.S.(1992),LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh. Biotechnology letters 14,6, 531-534[Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive]

Heidemann, E.(1997),Rechargeable Battery Operated Cooling Folding Electric Fan.Leder 48,(5),99-104[m.rfidfs.net]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price]

Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve.21.5′′ Wall Mount Display Capacitive Touch Monitor. USP/SP[Cog Graphic FPC LCD Display Module]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pagoda Gazebo Tent in Guangzhou for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[hongraygloves.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc]

Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Eyes- Caring LED Night Light for Nursing Reading with Soft Light]

Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[24 Volt Fire Alarm Strobe Light Horn Strobe Sounder]

Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Black Small Fridge for Showcase, Part I.Microwave Motion Sensor Module Doppler Radar Detector (HW-M10). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[YAMAHA Yv100II Nozzle Horder Square Head Short Type]

Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Zircon Butterfly Open Ring Diamond Fashion Woemn Rings.High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation, 175-184[High Level Application Waterproof Plastic Box]

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Whitaker,J.R.(1963),Spare Parts Custom Injection Plastic Moulding Manufacturer. Analytical Chemistry35(12), 1950-1953[Corrugated Board Production Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

T90 High Power Latching Relay Mini Power RelayAuthor for correspondence