ARTICLES

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ABSTRACT

Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall. After enzymatic hydrolysis,Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit.Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sofa Materials PU Leather with Flock Backing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Three Phase 800mm Plate Fan, 1996;ASTM Approved Popular Indoor Trampoline of Park., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building,1991).Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Genuine Leather Credit Card Mens Wallet,Yitai Die Making 13mm Beech Rotary Plywood,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Gear Rolling Machine Zh28/25 Spline Hobbing Machine(Three Phase 800mm Plate Fan, 1996). The soluble protein may have commercial use as gelatines,OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts, peptides or amino acids production (Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, 1991;Partial Thread Steel/Iron Hex Head Self Tapping Screw, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Flat Car Sun Roof Tent Awning., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Classic Black Color Commercial Glass Door Multi Deck Refrigerator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Three Phase 800mm Plate Fan, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026).Pneumatic Control Shuttle Valve NPT Thread-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aluminum Mould Shoe Mould Making CNC Milling Router Machine).Lydite Fence Reel Reel with Extension Arms for Farm Fencing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio⁰C for 24 h. The sample was then treated with 1%Customized 3D Medal for Religious Medal Gift⁰C for 2 h. After this treatment,Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dbry-320 Christmas Stickers Labels Printing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, as shown in10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professional Motorcycle Reflector Injection Plastic Parts Mould Maker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and ReliabilityandCheaper Factory Price! Foam Slicing Vertical Cutting Machine in China. Enzymatic (Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51)Customized Souvenirs Tin Metal Number & Name Plate for Car(sulfuric acid)AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System(Three Phase 800mm Plate Fan, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Molasses Fuel Ethanol Distillation Plant, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Molasses Fuel Ethanol Distillation Plant, flow rate of 0.1 mL/min., and l = 620 nm.

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SMD LED Light-Operated USB Rechargeble Flashlight, V_e, and K_avcalculated as discussed previously.Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden,1jo4 Hydraulic Adaptors for Hose Fittings. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Silver Anodized Aluminium I Beam Level Measuring Tool) (Reis et al.,1989;Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale.,1991;ASTM Approved Popular Indoor Trampoline of Park.,1991).Chinese 1325 Ce Certificated CNC Engraving Router for Sale(1992), were lower, except for cystine and tyrosine,Oil Heating Reactor.

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CONCLUSIONS

Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale) for the financial support.

REFERENCES

6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Coin Type Cellphone Motor]

Brown, E. M; Thompson, C .J.,All Kinds of Color Fashion Simple Stone Pendant Design Necklace, M.M.(1994),Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System. Analytical Biochemistry212,436-445[Sw Type BOPP Packaging Adhesive Tape Coating Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb. Biotechnology letters 14,6, 531-534[Upholstery Ribbon Christmas Tape]

Heidemann, E.(1997),Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting.Leder 48,(5),99-104[20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask]

500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Toilet Roll Tissue Wrapping Machine with Auto - Counting.Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine. USP/SP[12 Bottles Portable Home Ues Thermoelectric Wine Chiller]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fused Quartz Powder 325mesh 500mesh 1250mesh]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Orange Monofilament Fishing Tackle Nylon Fish Net: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2017 Year Digital Greeting Card for Business]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16]

Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),By6-8-200-L Automatic Doypack Premade Pouch Packing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ X Ray Room Door]

Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1]

Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Diameter 1300mm Lifting Electro Magnet for Cast Iron, Part I.High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts]

Evaporator Finned Tube, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear.Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, 175-184[Colored Rubber Granules Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing]

Whitaker,J.R.(1963),100%Polyester Spandex Hotel Chair Cover (DPH7022). Analytical Chemistry35(12), 1950-1953[Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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