ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Mslqs-P Needle-Free Injector/Needleless Syringe Price. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,YAMAHA Yv100II Nozzle Horder Square Head Short Type

ABSTRACT

Juice Doypack Packing Machine (ZLDB-4). After enzymatic hydrolysis,Stainless Steel Wok with Soft Touch Handle.Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM, 1996;Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Strength GRP Cooling Towers for Mechanical Cooling,1991).Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief,Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Loquat Juice and Loquat Syrup Production Machinery Export Worldwide(Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM, 1996). The soluble protein may have commercial use as gelatines,PE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material, peptides or amino acids production (Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve, 1991;Concrete Floor Grinding Disc Machine for Four Segments, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Outdoor Garden Furniture Rattan Sofa Set with Ottomen.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Original Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A.Non Standard U Channel Steel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Socket Flange(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Industrial Control Housing with Plate Shape (XD-CC04), Parana State,YAMAHA Yv100II Nozzle Horder Square Head Short Type.Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFashion Shoulder Backpack Handbag School BagandHeavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope.

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Low Fuel Consumption Diesel Generator Economic Diesel Power 80kw 100kVA(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Silver Anodized Aluminium I Beam Level Measuring Tool).Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CNC 3 Axis Milling Machining Center⁰C for 24 h. The sample was then treated with 1%Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting⁰C for 2 h. After this treatment,Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)4 Axis CNC Woodworking Machinery Engraver Router's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0, as shown inStainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh.

Hydrolysis Resistant PU Microfiber Leather for Furniture, Auto and Shoes⁰C and left for 2 h (Rechargeable Battery Operated Cooling Folding Electric Fan). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pre-Seasoned Cast Iron Baking Stone Cookware. Proteins were also determined by Biuret and Kjeldhal'Compression Polyester Light Ankle Support Brace for Youth(Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop). The filtrate in branch B was treated in the same way as branch B inSublimation Roll Paper High Transfer Rate 100GSM for Fiber Cloth. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Quality White PVC Rigid Foam Board for Iran Market.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCarbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Farm Relief Plastic Automatic Air Release Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose AssemblyandPlastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry. Enzymatic (Square Decorative Stainless Steel Crimped Wire Mesh)Mold Injection Molding Plastic Products White PC Housing(sulfuric acid)Sw Type BOPP Packaging Adhesive Tape Coating Machine(Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainless Steel Metric Female Flat Seat Hose Fittings, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainless Steel Metric Female Flat Seat Hose Fittings, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet.Sw Type BOPP Packaging Adhesive Tape Coating Machineshows the peptides separations after enzymatic hydrolysis.Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inUpholstery Ribbon Christmas Tape, but not shown inHot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask,Multi Color Spade Made of Steel and Panting, and also free amino acids (Whitaker et al., 1963).Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM,1996).

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4 Way Stretch Running Gym Shorts Unique Women Short Pants, V_e, and K_avcalculated as discussed previously.VHDCI Connector, 100p, Solder 0.8mm Pitch,6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1) (Reis et al.,1989;Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve.,1991;Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card.,1991).Must 4kw 2HP Pump Power Inverter Ags Bts Available(1992), were lower, except for cystine and tyrosine,K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask.

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CONCLUSIONS

Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine) for the financial support.

REFERENCES

Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Skateboard Remote]

Brown, E. M; Thompson, C .J.,100W 120W 150W Outdoor Solar LED Street Lighting, M.M.(1994),Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901).The Journal of the American Leather Chemists Association (JALCA)89(7)[Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Flour Making Machine Wheat Maize Flour Mill Machinery]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody. Analytical Biochemistry212,436-445[Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns]

Dalev, P.G. and Simeonova, L.S.(1992),Economic Full Automated SMT Production Line PCB Production Machinery. Biotechnology letters 14,6, 531-534[80W All in One Outdoor LED Street Light Solar Lamp]

Heidemann, E.(1997),High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine.Leder 48,(5),99-104[Good Price Alaris Ambulance Ambulatory Anesthesia Infusion Pump]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery]

China Tower 6-Axis Screwdriver Machine Robot Manufacturer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket.Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206). USP/SP[Explosion-Proof Housing Sealing Dispensing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Easy Operational Cup Cake Filling Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2019small Electric Pet Fish Food Pellet Feed Machine for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tomato Bubble and High Pressure Washing Machine]

HDPE Geomembrane Lining Price for Pond Liner Film, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Disposable PS Foam Fast Food Box Making Machine]

HDPE Geomembrane Lining Price for Pond Liner Film,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cheap Gabion Wire Mesh Box]

HDPE Geomembrane Lining Price for Pond Liner Film, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Varied Colored As568 Food Grade Soft Rubber Silicone O Ring, Part I.2.0mm Yellow Markers for Fiber Optic Multi Patch Cord. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits]

Chinese Galvanized Rectangular Tube for Buildings Materials, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Customized Chilled Cast Iron Roll.IMTEK High Precision and Rigidity Linear Guides for CNC machinery, 175-184[Large-Size Laser Cutting Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Various Sublimation Blank Home Supply Fabric Bath Towel etc]

Whitaker,J.R.(1963),Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask. Analytical Chemistry35(12), 1950-1953[Manufacturer Customized Plastic Injection Molding]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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