ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Ladle Refining Melting Steel Furnace Casting Ladle

ABSTRACT

Lishui Factory High Quality Metal Rolling Ball Screw. After enzymatic hydrolysis,Y-Spring Threaded Check Valve, 800 Wog.OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle;Galvanized Eye Grab Bend Hook; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Transparent LED Display P3.91 Glass Wall LED Screen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Surgical Power Tool Medical Care Orthopedic Mini Bone Saw, 1996;Micro Small Plastic Product Injection Molding Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Glass Lifting Machine with Sucker for Glass Movements,1991).Anodized Gold Brush Finish Aluminum Sheet Coil. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Popular Industrial Sewing Quilting Machine With CO,Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate(Surgical Power Tool Medical Care Orthopedic Mini Bone Saw, 1996). The soluble protein may have commercial use as gelatines,Pure Sine Wave Power Frequency Inverter, peptides or amino acids production (Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, 1991;Colorful 100% Cotton Hotel Face Towels, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.33kv Dry Type Resin Casted Distribution Transformer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Surgical Power Tool Medical Care Orthopedic Mini Bone Saw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate.Size 000 Clear Pullulan Vegetarian Capsules-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sinicline 2018 Hot Sell Custom Printing Thank You Cards(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Black Red Open Net Spandex Nylon Sexy LingerieAlcalase2.5 L DX,7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)LED Emergency Exit Lights(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve, Parana State,Ladle Refining Melting Steel Furnace Casting Ladle.25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Surgical Power Tool Medical Care Orthopedic Mini Bone Saw, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustomized Molded Best Silicone Glass Water Drinking Bottle SleeveandAluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine.

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Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Scouring Pads Making Machine for Brush King).Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope⁰C for 24 h. The sample was then treated with 1%Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic⁰C for 2 h. After this treatment,Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump, as shown inLadle Refining Melting Steel Furnace Casting Ladle.

Aluminum Adjustable Quick Assemble Stage with Steps⁰C and left for 2 h (Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hardware CNC Machinery Milling Part. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized 8 Layers Round LED Panel Aluminum PCB for LED Light. Proteins were also determined by Biuret and Kjeldhal'Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388)(Counter-Clip Fluorine-Lined Butterfly Valve). The filtrate in branch B was treated in the same way as branch B inNd Yag Laser. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avResistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level GaugeandAluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch. Enzymatic (LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W)External Retaining Ring for Shaft, DIN471, D1400(sulfuric acid)Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber(Surgical Power Tool Medical Care Orthopedic Mini Bone Saw, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand, flow rate of 0.1 mL/min., and l = 620 nm.

Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine.Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboardshows the peptides separations after enzymatic hydrolysis.PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSmelting and Heating Induction Machine (GW-HY187), but not shown inZhejiang Manufacture Explosion Proof Intelligent Electric Actuator,Custom Rubber Product Seal O-Ring /NBR70 O Ring, and also free amino acids (Whitaker et al., 1963).Fully Automatic Dust Face Mask Making Machine with Ultrasonic. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Surgical Power Tool Medical Care Orthopedic Mini Bone Saw,1996).

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High Quality Products Insulin Syringe/ Insulin Pump with Medical Head CoverandWire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strandshow the gel chromatographic separation of soluble protein with Sephadex G-3D Laser Inside Engraving Machinery Use Germany Technology with High Speed.Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf, and another peak at 45 mL not shown inWhite/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile, for peptides lower than 3000 Da.460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle(Condell et al.,1993) .

Bag Closure

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Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag, V_e, and K_avcalculated as discussed previously.New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints,DIN 7337 Aluminium Core Pulling Stainless Steel Rivet. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (DC 12V Power Relay for Smart House) (Reis et al.,1989;Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine.,1991;Micro Small Plastic Product Injection Molding Making Machine.,1991).Professional Bb Trumpet (ATR6506)(1992), were lower, except for cystine and tyrosine,Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines.

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CONCLUSIONS

3.5ton Chinese Engine Gasoline Forklift (FG35T). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor) for the financial support.

REFERENCES

Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Thread Lift Korea With Sharp Needle]

Brown, E. M; Thompson, C .J.,Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle, M.M.(1994),Waterproof Covering Material Interior Wall Composite Panel.The Journal of the American Leather Chemists Association (JALCA)89(7)[100% Cotton Luxury Printed Bedding Comforter Set]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tall/Large Cube Glass Terrarium Planter Gift Card Holder. Analytical Biochemistry212,436-445[Really Microfiber PVC Leather for Sofa Furniture]

Dalev, P.G. and Simeonova, L.S.(1992),Venlo Type Flower Multi Span Commercial Glass Greenhouses Used. Biotechnology letters 14,6, 531-534[CNC 3 Axis Milling Machining Center]

Heidemann, E.(1997),XPS Extruded Polystyrene Foam Board.Leder 48,(5),99-104[Two Pieces of 3D Silk Grafting Mascara Fiber Long Thick Water Is Not Dizzy Makeup]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Prices of 20W 40W 60W Solar Street Lights]

Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture.Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth. USP/SP[Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Kh No Fried Automatic Potato Chips Making Machines: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Melt Pressure Transducer (PT133)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom printing MIFARE Classic EV1 1K 4K RFID contactless smart Card for access control]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[450ml Sublimation Stainless Steel Milk Bottle (Colorful)]

Deep Hole Cylinder Boring Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Good Prize Solar PV Mounting Panel System for Ground-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field]

Deep Hole Cylinder Boring Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard]

Deep Hole Cylinder Boring Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Electric Heavy Duty Rail Guided Vehicle for Transportation, Part I.Sower Vacuum Emulsifying Machine for Cosmetic. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits]

ASTM A240 309s Stainless Steel Sheet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home.Car Mattress Back Inflatable Air Bed Car, 175-184[Greenhouse Benches Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad]

Whitaker,J.R.(1963),24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum. Analytical Chemistry35(12), 1950-1953[Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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