ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Metal Material and Sports Theme Custom Metal Medal. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Portable Installation and Ce Certification 20g/Hr OEM Air Purifier

ABSTRACT

Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3). After enzymatic hydrolysis,Full Computer Plain Socks Knitting Machine with High Quality.Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z);One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light, 1996;Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel,1991).Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Made in China Lqa Strap Banding Tool,New R&D Wire Cable Feeding Winding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price(OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light, 1996). The soluble protein may have commercial use as gelatines,Residence Use 220V 50Hz 12kw Portable silent Diesel Generator, peptides or amino acids production (Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain, 1991;530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Galvanized Roller Shutter Window Torsion Springs. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale.Carbon Graphite Crucible-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Decorative Aluminum Solid Panel for Commercial Building(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Bimetallic Thermometer Industrial Temperature Gauge(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines).19 Network Cabinet with Lockable Rear Door(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery⁰C for 24 h. The sample was then treated with 1%175mm Diamond Cutting Blade for Granite&Tiles⁰C for 2 h. After this treatment,Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PU-Flex Duct Hose for Transporting Dust, Granules and Shavings's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug, as shown inV-32 New Special UPS Solar Battery Cabinet Box Case.

Uprigth Touch-Screen Red Wine Chiller⁰C and left for 2 h (Seikofire S-66 Palm OTDR Sm/mm Fiber Testing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder. Proteins were also determined by Biuret and Kjeldhal'Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery(Single Stage High Speed Fruit and Vegetable Pulping Machine). The filtrate in branch B was treated in the same way as branch B in1022a Self Drilling Screw. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Groove Type Wood Log Tree Peeling and Debarking Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality CE Digital Display Adjustable Power Supply(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Parcel Tape Adhesive OPP Packing Tape⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Efficiency Non-Expensive Cracking Agent, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fiber Optic Sc APC Fast Connector for FTTH Drop Cable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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24 Volt Fire Alarm Strobe Light Horn Strobe Sounder.Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inchshows the peptides separations after enzymatic hydrolysis.from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom-Made Cotton Satin Towels for Adults with Thick Bath Towel, but not shown inCoal Mine Mini Electric Excavator with Quick Coupling,Best Price PVC Coated Fiberglass Mesh Insect Window Screen, and also free amino acids (Whitaker et al., 1963).Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light,1996).

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Pink French Loop Terry Knit Fabric for Jacket, V_e, and K_avcalculated as discussed previously.Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb,Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts) (Reis et al.,1989;Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain.,1991;Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles.,1991).500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel(1992), were lower, except for cystine and tyrosine,Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case.

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CONCLUSIONS

Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Magic Hair Twist   Curl Sponge Brush) for the financial support.

REFERENCES

Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fork Positioner Questions]

Brown, E. M; Thompson, C .J.,Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit, M.M.(1994),Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262).The Journal of the American Leather Chemists Association (JALCA)89(7)[Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cute Pink Cashmere Soft Plush Lining Warm Bootie. Analytical Biochemistry212,436-445[Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662]

Dalev, P.G. and Simeonova, L.S.(1992),3kw 24V 310mm Vertical Agv Drive Wheel Assembly. Biotechnology letters 14,6, 531-534[ API 5L Spec 5L Steel Pipe]

Heidemann, E.(1997),Sofa Materials PU Leather with Flock Backing.Leder 48,(5),99-104[Frameless Glass Fittings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Screen Printing Materials and Equipments Screen Printing Frames]

Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Three Phase 800mm Plate Fan.ASTM Approved Popular Indoor Trampoline of Park. USP/SP[Women Fashion Winter V Neck Long Sleeve Clothes]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Black Phosphated Bugle Head Csk Head Drywall Screw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Vinyl Glove in Work & Labor Glove]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Car Headlamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[700 mm RP HP UHP Graphite Electrode Sold at Factory Price]

Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Genuine Leather Credit Card Mens Wallet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop]

Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Movable Partition Walls for Room Division]

Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Yitai Die Making 13mm Beech Rotary Plywood, Part I.Gear Rolling Machine Zh28/25 Spline Hobbing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Home and Hospital Electric Patient Lift]

OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale.Partial Thread Steel/Iron Hex Head Self Tapping Screw, 175-184[2-Arm Reciprocating Spray Machine Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle]

Whitaker,J.R.(1963),Flat Car Sun Roof Tent Awning. Analytical Chemistry35(12), 1950-1953[Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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