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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Two Roll Rubber & Plastic Mixing Mills Tire Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes

ABSTRACT

Hot Sale 25kw Grid Tied Home Solar Panel System Kit. After enzymatic hydrolysis,Mini Car Portable Fridge 10L Mini Fridge.Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Mini Small Garden Excavator Digging Work;Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Offshore Drilling Platform Air Winch for Marine Oilfields, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mass Stock Yl10.2 Carbide Rods for Milling 45HRC, 1996;Heavy Industry Electric Transport Vehicle on Rails., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Manual Interlocking Brick Making Machine,1991).A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing,Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile(Mass Stock Yl10.2 Carbide Rods for Milling 45HRC, 1996). The soluble protein may have commercial use as gelatines,Metal Gold Marathon Running Sport Medal for Promotion Gift, peptides or amino acids production (12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery, 1991;Security Shopping Trolley Baby Seat for Supermarket Cart, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mass Stock Yl10.2 Carbide Rods for Milling 45HRC, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine.Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Manual Foot Pedal Hydraulic Pump(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CH Series Pid Intelligent Temperature Controller CH402).Stainless Steel Mechanic Parts for Lock Accessories with MIM Process(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT⁰C for 24 h. The sample was then treated with 1%Mining Machinery Sand Vibrating Sieve for River Sand⁰C for 2 h. After this treatment,Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PRO Audio Loudspeaker PA Speaker System Line Array Speaker's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Palet Coil Nail Welding Machine/Nail Making Machine, as shown inFashion Metal Button Crystal Shank Button for Garment.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%10A MPPT Solar Controllers PV 60V for off-Grid. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.60ml 100ml Customized Glass Jar for Spice, Candle, Candy⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Diesel Generator Parts AVR Ki-Davr-95s for Kipor.Galvanized Eye Grab Bend Hookshows the peptides separations after enzymatic hydrolysis.Gld Brand Padlock Brass Popular in 5 Continents  (Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChinese Galvanized Rectangular Tube for Buildings Materials, but not shown inUF Membrane Aqu160 with Competitive Price and High Quality,No Lead Bronze Flange Manufacturer, and also free amino acids (Whitaker et al., 1963).Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Mass Stock Yl10.2 Carbide Rods for Milling 45HRC,1996).

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Laptop Bag College Men Backpack Leather Back Pack Bags, V_e, and K_avcalculated as discussed previously.Pet Dog Grooming Rake Dematting Tool Shedding Comb,Custom Apparel Applique Round Patch School Club Embroidery Collar Patch. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose) (Reis et al.,1989;12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery.,1991;Heavy Industry Electric Transport Vehicle on Rails.,1991).Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery(1992), were lower, except for cystine and tyrosine,CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058.

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CONCLUSIONS

Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CH Series Pid Intelligent Temperature Controller CH402) for the financial support.

REFERENCES

Automatic Food Metal Detector Machine for Production Line, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Truck Parts Cylinder Block for Caterpillar 3306 1n3576 4p623 3304/3066/S6K/320/3116]

Brown, E. M; Thompson, C .J.,Multi-Functional Backpack Manual 52cc Brush Cutter, M.M.(1994),Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa.The Journal of the American Leather Chemists Association (JALCA)89(7)[Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine. Analytical Biochemistry212,436-445[Ss Reduced Port 2000wog 1PC Screwed Ball Valve]

Dalev, P.G. and Simeonova, L.S.(1992),Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm. Biotechnology letters 14,6, 531-534[Residential Reverse Osmosis 0.0001um Water Filter Water Filtration]

Heidemann, E.(1997),White Cotton Satin Hotel Duvet Cover (DPFB8055).Leder 48,(5),99-104[Sports Travel Outdoor Shoulder Duffel Hand Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1jo4 Hydraulic Adaptors for Hose Fittings]

79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Small Cleaning Brush ESD Anti Static Brush ESD Brush Black.PAC /Poly Aluminium Chloride for Water Treatment. USP/SP[Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminum Sheet Shearing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Event woven cloth ICODE SLIX RFID stretch elastic wrist band]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electric Fireplace PCBA with Double Layers Circuit Board]

Electrical PV DC Solar Cable, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Tomato Bubble and High Pressure Washing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Plastic Injection Moulding Machine Supplier / Factory /Manufacturer]

Electrical PV DC Solar Cable,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder]

Electrical PV DC Solar Cable, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Upholstery Ribbon Christmas Tape, Part I.Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Whole Set Gas Spring Strut for Furniture]

2+1 Flavors Soft Ice Cream Machine with Pre-Cooling, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver.40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse, 175-184[Stainless Steel Forging Ring]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Union Metal Steel Building and Construction Material]

Whitaker,J.R.(1963),Best Sale Low Price Household Air freshener Spray. Analytical Chemistry35(12), 1950-1953[Factory Direct Sale Single Cloth Hanger Hook]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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