ARTICLES

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ABSTRACT

High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags. After enzymatic hydrolysis,Forged Steel Pressure Sealed Bonnet Globe Valve.Plastic Injection Moulding Machine Supplier / Factory /Manufacturer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (80W All in One Outdoor LED Street Light Solar Lamp, 1996;Hot Sale Safety Vinyl PVC Flooring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation,1991).Horizental Stainless Steel Food Grade Milk Tank for Milking. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Multi Parameter Small Digital Domestic Water Meter TDS Meter,27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sanhe FC-2 Poultry Air Vent(80W All in One Outdoor LED Street Light Solar Lamp, 1996). The soluble protein may have commercial use as gelatines,Picosecond Laser Tattoo Removal Skin Care Medical Instrument, peptides or amino acids production (XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale, 1991;Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.SUS316 Hexagon Socket Set Screws with Cone Point, DIN914.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (80W All in One Outdoor LED Street Light Solar Lamp, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor.Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Explosion-Proof Housing Sealing Dispensing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo), Parana State,Biomass Drum Wood Chipper with Competitive Price.Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (80W All in One Outdoor LED Street Light Solar Lamp, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC FilmandElectric Fireplace PCBA with Double Layers Circuit Board.

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Rear Wheel Exchangeable Aluminum Manual Wheelchair(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder).90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Graphic 128X128 Dots Cog LCD⁰C for 24 h. The sample was then treated with 1%Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen⁰C for 2 h. After this treatment,Tcn Sex Toy Vending Machines for Sale S800-10. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Silver 8 Inch Double Thick Round Cake Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Precision Machining Auto Parts Metal Contact, as shown inWhole Set Gas Spring Strut for Furniture.

Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label⁰C and left for 2 h (Union Metal Steel Building and Construction Material). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Paper Sticky Lint Remover Roller/Pet Hair Roller. Proteins were also determined by Biuret and Kjeldhal'Safe Durable Scaffolding Trapdoor Plank for Construction(Factory Direct Sale Single Cloth Hanger Hook). The filtrate in branch B was treated in the same way as branch B in350g Cheap Yellow Soap for Laundry/ Clothes Bar Soap. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Various Sublimation Blank Home Supply Fabric Bath Towel etc. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Quality Bending Metal Mould with All Kinds Metal Materials, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1). Band Resaw for Lumbershows the peptides separations after enzymatic hydrolysis.Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSi3n4 Full Ceramic  Bearing  608 Size 8X22X7, but not shown inHigh Qualified Medical Grade Thin Wall Stainless Steel Tube,Food Machine Cube Ice Machine for Commercial Use, and also free amino acids (Whitaker et al., 1963).Christmas Decoration Rose Flower LED Light String. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (80W All in One Outdoor LED Street Light Solar Lamp,1996).

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Granules and Shavings

Dxz Limit Switch Tower Crane Spare Parts, V_e, and K_avcalculated as discussed previously.OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock,High-End Business Magnet Leather Ring Binder Notebook. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate) (Reis et al.,1989;XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale.,1991;Hot Sale Safety Vinyl PVC Flooring.,1991).Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops(1992), were lower, except for cystine and tyrosine,New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace.

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CONCLUSIONS

Eyes- Caring LED Night Light for Nursing Reading with Soft Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Car Badge Custom Emblem Auto Logo Sign for Advertising) for the financial support.

REFERENCES

China Bearing with Housing Bearing / Pillow Block Bearing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2019 36*10W RGBW LED Moving Head Stage Lighting for DJ Disco Club]

Brown, E. M; Thompson, C .J.,Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800, M.M.(1994),The Most Competitive Sodium Gluconate for Water Reducer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Trunk Organizer Foldable Water Proof]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles. Analytical Biochemistry212,436-445[Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe]

Dalev, P.G. and Simeonova, L.S.(1992),Electric Magic Hot Hair Styling Curling Iron with Brushes. Biotechnology letters 14,6, 531-534[Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular]

Heidemann, E.(1997),Melt Pressure Transducer (PT133).Leder 48,(5),99-104[32 Inch TV Lens Strips 6 LED for LG Brand]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[304 310 Stainless Steel Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth]

Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Air Bubble Insulation Thermal Building Insulation Material]

Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer]

Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord, Part I.Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels]

Durable Aluminum Flag Pole with Dome Top Tangle Free Finial, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized Marathon Medal for Marathon Medallion.Price of a Retail Supermarket Push Cart for Sale, 175-184[Erw Galvanized Rectangular Tube]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Various Colors Nylon and Stainless Steel Kitchen Tools]

Whitaker,J.R.(1963),Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder. Analytical Chemistry35(12), 1950-1953[High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Factory Outlet Automatic Grain Tower Dryer Drying EquipmentAuthor for correspondence