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ABSTRACT

90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow. After enzymatic hydrolysis,Graphic 128X128 Dots Cog LCD.Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Precision Machining Auto Parts Metal Contact, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label, 1996;Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Paper Sticky Lint Remover Roller/Pet Hair Roller,1991).Safe Durable Scaffolding Trapdoor Plank for Construction. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display,Various Sublimation Blank Home Supply Fabric Bath Towel etc,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic(Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label, 1996). The soluble protein may have commercial use as gelatines,Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments, peptides or amino acids production (Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, 1991;Best Quality Bending Metal Mould with All Kinds Metal Materials, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Milling Precision Stainless Steel CNC Machinery Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Good Price Stand-up Zipper Doy Pouch Filling Packing Machine.Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

D Section Stainless Steel Tube, Parana State,Rear Wheel Exchangeable Aluminum Manual Wheelchair.Mint Flavor 50ml Home Foam Toothpaste, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBelt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors LowandStainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film.

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125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plastic Injection Moulding Machine Supplier / Factory /Manufacturer).Dxz Limit Switch Tower Crane Spare Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock⁰C for 24 h. The sample was then treated with 1%High-End Business Magnet Leather Ring Binder Notebook⁰C for 2 h. After this treatment,Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Eyes- Caring LED Night Light for Nursing Reading with Soft Light, as shown inAutomatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder.

China Bearing with Housing Bearing / Pillow Block Bearing⁰C and left for 2 h (Whole Set Gas Spring Strut for Furniture). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,The Most Competitive Sodium Gluconate for Water Reducer. Proteins were also determined by Biuret and Kjeldhal'Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles(Union Metal Steel Building and Construction Material). The filtrate in branch B was treated in the same way as branch B inProfessional Body Tightening Machine Skin Care Radar V-Max Hifu Face Lifting Beauty. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electric Magic Hot Hair Styling Curling Iron with Brushes.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Melt Pressure Transducer (PT133). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGalvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder.27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Displayshows the peptides separations after enzymatic hydrolysis.Lady Kids Child Yoga Clothes Gym Fitness Sports Wear(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Band Resaw for Lumber, but not shown inSi3n4 Full Ceramic  Bearing  608 Size 8X22X7,Hotel Steel Digital Room Safes with Large LED Display, and also free amino acids (Whitaker et al., 1963).Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label,1996).

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Fruit Plum Cleaning Washing Machine with Brushes SprayandPlastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storageshow the gel chromatographic separation of soluble protein with Sephadex G-Biogas/Methane Purification System/Desulfurization/Scrubber Tower.Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line, and another peak at 45 mL not shown inPromotional Plastic Laminated Custom Shopping PP Woven Bag, for peptides lower than 3000 Da.Popular Custom School Uniform Woven Badge Patch with Merrow Border(Condell et al.,1993) .

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1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor, V_e, and K_avcalculated as discussed previously.Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter,Electrical Stationary Hoist Tower Crane 500kg Motor. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Granules and Shavings) (Reis et al.,1989;Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube.,1991;Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler.,1991).Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage(1992), were lower, except for cystine and tyrosine,Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange.

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CONCLUSIONS

Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Food Machine Cube Ice Machine for Commercial Use) for the financial support.

REFERENCES

Flow Wrapper/ Bakery Moon Cake Packing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Products Suppliers Camlock Coupling Quick Couplings Female]

Brown, E. M; Thompson, C .J.,Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine, M.M.(1994),Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reusable Metal Stainless Steel Drinking Straws. Analytical Biochemistry212,436-445[Trunk Organizer Foldable Water Proof]

Dalev, P.G. and Simeonova, L.S.(1992),Designed Steel Frame Factory Building. Biotechnology letters 14,6, 531-534[Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe]

Heidemann, E.(1997),Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate.Leder 48,(5),99-104[Professional Manufacturer of Stainless Steel304/316 Wire Rope]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular]

Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table.Scouring Pads Making Machine for Brush King. USP/SP[2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Powder/Spices Powder Packing Machine with Four/Three Side Seal]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Auto Key Cutters]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine]

Glass Mix Stone Mosaic Tiles, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth]

Glass Mix Stone Mosaic Tiles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Air Bubble Insulation Thermal Building Insulation Material]

Glass Mix Stone Mosaic Tiles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, Part I.Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer]

Used/Second Hand 120mpm Continous Galvanizing Line/Cgi, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie.Racing Preformance PTFE Teflon Alloy Fuel Adapter, 175-184[insharevape.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels]

Whitaker,J.R.(1963),Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170. Analytical Chemistry35(12), 1950-1953[Various Colors Nylon and Stainless Steel Kitchen Tools]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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