ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Asco 353 Series 3/4 Pulse Valve Solenoid Valve. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material

ABSTRACT

Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle. After enzymatic hydrolysis,Purple UL 1095 16AWG Electronic Lead Wire.Brushed Anodizing Aluminum Extrusion Profile for LED. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps;S235jo S235jr Steel Pipe Tube Shs Rhs; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pre-Seasoned Cast Iron Mini Oval Casserole Pot, 1996;Stainless Steel Square Wire Mesh Netting with SGS., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter,1991).Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Outdoor Garden Save Energy with Solar Flame Wall Light,CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag(Pre-Seasoned Cast Iron Mini Oval Casserole Pot, 1996). The soluble protein may have commercial use as gelatines,Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length, peptides or amino acids production (Non Stick Silicone Coated Fiberglass Cloth, 1991;Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pre-Seasoned Cast Iron Mini Oval Casserole Pot, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr.White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale 100% Polyester Cartoon Kids Sleepwear(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Diaphragm Valve for Bag Filter Since 1992, Parana State,China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material.4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pre-Seasoned Cast Iron Mini Oval Casserole Pot, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLow Pressure PU Filter Gasket Sandal Making MachineandPortable Telescopic Straw Metal Drinking Straw with Brush Case.

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Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain).Manufacturer Plastic Injection Mould Molding Nylon PP Injection(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe⁰C for 24 h. The sample was then treated with 1%High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack⁰C for 2 h. After this treatment,Aluminum Forging Cast Metal Sand Die Casting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)ADC12 Aluminum Die Casting Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale, as shown inMetal Galvanized Wave Form Perforated Steel Strips.

Custom Logo Cotton Terry Oversize Pullover Men Hoodie⁰C and left for 2 h (Best Price PVC Coated Fiberglass Mesh Insect Window Screen). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer. Proteins were also determined by Biuret and Kjeldhal'Seikofire S-66 Palm OTDR Sm/mm Fiber Testing(43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump). The filtrate in branch B was treated in the same way as branch B inAuto Engine Part 4y Cylinder Head for Toyota Engine 11101-73020. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PVC Fabric Rapid Roller Shutter for Clearn Room.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fiberglass Animal Poultry Insulation Grain Tower. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCraft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale 375ml Dark Green Glass Ice Wine Bottles⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

3FT 3000lm IP66 ApprovedandCog Graphic FPC LCD Display Module. Enzymatic (Power Fitting- Hot DIP Galvanizing Bolt with Nut)Slewing Gear for Tower Crane Slewing Winch Reducer(sulfuric acid)Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine(Pre-Seasoned Cast Iron Mini Oval Casserole Pot, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes.New Swim Shorts Grey Mens Board Shorts with Mesh Linershows the peptides separations after enzymatic hydrolysis.Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPetrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800, but not shown in6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube,Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8), and also free amino acids (Whitaker et al., 1963).Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pre-Seasoned Cast Iron Mini Oval Casserole Pot,1996).

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Type Of Firework

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CONCLUSIONS

High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Laparoscopy Clip Applicator for Abdominal Surgery Uses) for the financial support.

REFERENCES

Precision Customized OEM Auto Hardware Lathe CNC Machining Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Digital Fireproof Safe Box]

Brown, E. M; Thompson, C .J.,White Color Enamel Bowls with High Quality, M.M.(1994),Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plant Stands & Accessories Glass Hanging Flower Pot Bracket]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Electric Magic Hot Hair Styling Curling Iron with Brushes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line. Analytical Biochemistry212,436-445[21.5′′ Wall Mount Display Capacitive Touch Monitor]

Dalev, P.G. and Simeonova, L.S.(1992),Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board. Biotechnology letters 14,6, 531-534[Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125]

Heidemann, E.(1997),Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration.Leder 48,(5),99-104[Coated Fiberglass Cloth Silver Gray Silicon]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer]

Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Southeast Asia Healthy Soft Integrated Latex Foam Mattress.12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad. USP/SP[Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PVC Cabinet Construction Board]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Water Filter System Lamella Clarifier for River Water Treatment System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Small Cross Body Bags]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Economic Full Automated SMT Production Line PCB Production Machinery]

Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cabinet Shelf Support Pin Furniture Hardware Fittings-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine]

Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle]

Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Reasonable Price High Quality Nylon Cable Gland, Part I.Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality CO2 Laser Marking Machine with Ce]

Disposable Anesthesia Breathing Circuit Tube, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type.Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element, 175-184[pulpmask.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel HK Series Needle Roller Bearing HK0408]

Whitaker,J.R.(1963),New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126. Analytical Chemistry35(12), 1950-1953[Original Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light FittingAuthor for correspondence