ARTICLES

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ABSTRACT

Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002). After enzymatic hydrolysis,By6-8-200-L Automatic Doypack Premade Pouch Packing Machine.Diameter 1300mm Lifting Electro Magnet for Cast Iron. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%;Evaporator Finned Tube; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, 1996;100%Polyester Spandex Hotel Chair Cover (DPH7022)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Supply Perforated Metal Mesh,1991).CNC Turning Center, CNC Horizontal Turning Milling Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16,Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Classical Garment Accessories Jeans Button Metal Shank Button(Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, 1996). The soluble protein may have commercial use as gelatines,Vitamin D3 Fat-Solubility Water-Solubility Feed Additive, peptides or amino acids production (Factory Wholesale Zinc Plated Carbon Steel Hex Nut, 1991;Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Cheese Making Machine Electric.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰100W Dimmable LED Corn Bulb with Meanwell Driver.Pet and Animal Food Extrusion Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Biomass Drum Wood Chipper with Competitive Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Whole Set Gas Spring Strut for Furniture).High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Forged Steel Pressure Sealed Bonnet Globe Valve⁰C for 24 h. The sample was then treated with 1%Plastic Injection Moulding Machine Supplier / Factory /Manufacturer⁰C for 2 h. After this treatment,Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure, as shown inUnion Metal Steel Building and Construction Material.

80W All in One Outdoor LED Street Light Solar Lamp⁰C and left for 2 h (Factory Direct Sale Single Cloth Hanger Hook). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Safety Vinyl PVC Flooring. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation. Proteins were also determined by Biuret and Kjeldhal'Horizental Stainless Steel Food Grade Milk Tank for Milking(27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display). The filtrate in branch B was treated in the same way as branch B inCages For Quail Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Multi Parameter Small Digital Domestic Water Meter TDS Meter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSanhe FC-2 Poultry Air Vent(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Band Resaw for LumberandSUS316 Hexagon Socket Set Screws with Cone Point, DIN914. Enzymatic (Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale)Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor(sulfuric acid)Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC(Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Explosion-Proof Housing Sealing Dispensing Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Explosion-Proof Housing Sealing Dispensing Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Tube Mills for Thin Wall Tubing.Si3n4 Full Ceramic  Bearing  608 Size 8X22X7shows the peptides separations after enzymatic hydrolysis.Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Qualified Medical Grade Thin Wall Stainless Steel Tube, but not shown inFruit Plum Cleaning Washing Machine with Brushes Spray,Car Badge Custom Emblem Auto Logo Sign for Advertising, and also free amino acids (Whitaker et al., 1963).2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine,1996).

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CONCLUSIONS

High Precision Machining Auto Parts Metal Contact. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (4-14 Manual Staple Gun with Staples for Hand Tools) for the financial support.

REFERENCES

Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Women Waterproof Raincoat Outdoor Hooded Rain Jacket]

Brown, E. M; Thompson, C .J.,Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler, M.M.(1994),Paper Sticky Lint Remover Roller/Pet Hair Roller.The Journal of the American Leather Chemists Association (JALCA)89(7)[Trunk Organizer Foldable Water Proof]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Safe Durable Scaffolding Trapdoor Plank for Construction. Analytical Biochemistry212,436-445[Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular]

Dalev, P.G. and Simeonova, L.S.(1992),China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display. Biotechnology letters 14,6, 531-534[2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank]

Heidemann, E.(1997),Various Sublimation Blank Home Supply Fabric Bath Towel etc.Leder 48,(5),99-104[1 Inch Lcd Module Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Powder/Spices Powder Packing Machine with Four/Three Side Seal]

Toilet Roll Tissue Wrapping Machine with Auto - Counting, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic.Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments. USP/SP[OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PS Foam Disposable Fast Food Box Making Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Air Bubble Insulation Thermal Building Insulation Material]

Best Quality Bending Metal Mould with All Kinds Metal Materials, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer]

Best Quality Bending Metal Mould with All Kinds Metal Materials,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels]

Best Quality Bending Metal Mould with All Kinds Metal Materials, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Milling Precision Stainless Steel CNC Machinery Parts, Part I.U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Various Colors Nylon and Stainless Steel Kitchen Tools]

Good Price Stand-up Zipper Doy Pouch Filling Packing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21.Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10, 175-184[Plastic Mold Injection Mould Molding Factory Spare Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325)]

Whitaker,J.R.(1963),360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1). Analytical Chemistry35(12), 1950-1953[Factory Outlet Automatic Grain Tower Dryer Drying Equipment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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