ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Plastic Caps Making Machine / Injection Molding Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate

ABSTRACT

Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge. After enzymatic hydrolysis,Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness.LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Micro DC 24V Worm Gear Brushless Motor for Medical Equipment, 1996;1.6 Ton Hand Lifting Tool Lever Chain Block., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CNC Machining Service High Precision CNC Mechanical Parts,1991).Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker,Printed 100% Polyester Tcheap Wholesale Blankets,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Food Grade Material 304/316 Double Diaphragm Pumps(Micro DC 24V Worm Gear Brushless Motor for Medical Equipment, 1996). The soluble protein may have commercial use as gelatines,Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels, peptides or amino acids production (FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator, 1991;High Power From Han′s GS 2000W Laser Cutter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Widely Application Latest Designed Coffee Capsule Filling Packing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Micro DC 24V Worm Gear Brushless Motor for Medical Equipment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰18V Lithium Battery Powered Cordless Drill.High Density Rock Wool Wall Board with Thermal Insulation Performance-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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6 Inch Plastic Breathing Cap for The IBC Valve⁰C and left for 2 h (Custom Aluminum Cover Frame Half Aluminum Cover Profile). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging. Proteins were also determined by Biuret and Kjeldhal'Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree(Low Fuel Consumption Diesel Generator Economic Diesel Power 80kw 100kVA). The filtrate in branch B was treated in the same way as branch B inMolecular Sieve 5A for Psa Adsorber Oxygen Molecular Sieve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIridium Power Denso Spark Plug Bk6rei-11 for Honda Cars(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Freshwater Pearl Butterfly Flower Bride Hairpin⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Air Bubble Insulation Thermal Building Insulation Material, pH 3.25., mobile phase B: buffer solution of 0.01 mol/LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China PAC Water Flocculating Agent for Water Treatment) for the financial support.

REFERENCES

Wholesale Black Galvanized Head Stainless Steel Screw, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic Grip Wood Handle Shovel]

Brown, E. M; Thompson, C .J.,PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine, M.M.(1994),45*60cm Anti-Fatigue ESD Rubber Floor Mat.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Metal Galvanized Wave Form Perforated Steel Strips]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints. Analytical Biochemistry212,436-445[Best Price PVC Coated Fiberglass Mesh Insect Window Screen]

Dalev, P.G. and Simeonova, L.S.(1992),Vinyl Glove in Work & Labor Glove. Biotechnology letters 14,6, 531-534[43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump]

Heidemann, E.(1997),Low Energy Needed Compact Flour Mill Plant for Milling Wheat.Leder 48,(5),99-104[Solar Mc4 PV Connector with TUV Approval TUV Certificate Mc4 Connector]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ 3FT 3000lm IP66 Approved]

Cotton Yarn Computerized Lace Knitting Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Nylon Weatherproof PA6 Strain Relief Cable Gland.Steel Roller Embossed Paper 2019 New Design. USP/SP[New Swim Shorts Grey Mens Board Shorts with Mesh Liner]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.swinysteel.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Transparent Composite Fiberglass Roof Tiles]

Factory Price DIN5685 Galvanized Marine Anchor Link Chain, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine]

Factory Price DIN5685 Galvanized Marine Anchor Link Chain,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ with Handle Vegetable Oil Nonstick]

Factory Price DIN5685 Galvanized Marine Anchor Link Chain, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, Part I.High Level Application Waterproof Plastic Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Printed 100% Polyester Tcheap Wholesale Blankets]

Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse.Water Slide for Indoor Play Structure, 175-184[Oem Custom Golf Hat For Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[150kg Per Hour Frozen French Fries Production Line]

Whitaker,J.R.(1963),Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder. Analytical Chemistry35(12), 1950-1953[Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V)Author for correspondence