ARTICLES

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ABSTRACT

12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery. After enzymatic hydrolysis,Security Shopping Trolley Baby Seat for Supermarket Cart.Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use, 1996;Manual Foot Pedal Hydraulic Pump., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag,1991).Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CH Series Pid Intelligent Temperature Controller CH402,China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Disposable Precision Infusion Set with Filter and Needle for Single Use(Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use, 1996). The soluble protein may have commercial use as gelatines,Low Price Sweep Frequency Response Analyzer for Power Transformer Test, peptides or amino acids production (Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500, 1991;Stainless Steel Mechanic Parts for Lock Accessories with MIM Process, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mining Machinery Sand Vibrating Sieve for River Sand.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PRO Audio Loudspeaker PA Speaker System Line Array Speaker.Palet Coil Nail Welding Machine/Nail Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Uprigth Touch-Screen Red Wine Chiller).60ml 100ml Customized Glass Jar for Spice, Candle, Candy(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price⁰C for 24 h. The sample was then treated with 1%Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line⁰C for 2 h. After this treatment,Factory Outlet Automatic Grain Tower Dryer Drying Equipment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mesh Bag Packing 20kg Pure White Garlic's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Supreme 524 Mv Sea Kayak, as shown inCe Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain.

Trunk Organizer Foldable Water Proof⁰C and left for 2 h (Stainless Steel Clarifier Tank for Water Treatment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.100%Polyester Burnout Velvet Sofa Fabric (pineapple grid). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Diesel Generator Parts AVR Ki-Davr-95s for Kipor. Proteins were also determined by Biuret and Kjeldhal'Gld Brand Padlock Brass Popular in 5 Continents  (Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce). The filtrate in branch B was treated in the same way as branch B inWire Rope Pulley. The final soluble protein shown were used for peptides separations using gel permeation chromatography,No Lead Bronze Flange Manufacturer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSnsc Fd30 Side Shift New Diesel Forklift 3ton(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Laptop Bag College Men Backpack Leather Back Pack Bags, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pet Dog Grooming Rake Dematting Tool Shedding Comb, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Aluminum Sheet Shearing Machine, V_e, and K_avcalculated as discussed previously.Electrical PV DC Solar Cable,Tomato Bubble and High Pressure Washing Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side) (Reis et al.,1989;Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500.,1991;Manual Foot Pedal Hydraulic Pump.,1991).Upholstery Ribbon Christmas Tape(1992), were lower, except for cystine and tyrosine,Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate.

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CONCLUSIONS

2+1 Flavors Soft Ice Cream Machine with Pre-Cooling. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Supplier V Wedge Wire Johnson Water Well Screen) for the financial support.

REFERENCES

Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hydraulic 22t Gasoline Log Splitter]

Brown, E. M; Thompson, C .J.,40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse, M.M.(1994),Best Sale Low Price Household Air freshener Spray.The Journal of the American Leather Chemists Association (JALCA)89(7)[Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Supplied High Strength Marine Anchor Chain. Analytical Biochemistry212,436-445[Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF]

Dalev, P.G. and Simeonova, L.S.(1992),Humidity Refrigeration Digital Controller Jsd-100+. Biotechnology letters 14,6, 531-534[from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder]

Heidemann, E.(1997),Elegant Europe Dessign Pillow Top Mattress.Leder 48,(5),99-104[Hydraulic Drilling Jar]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Speed Cable Wire Winding Braiding Machine]

Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8).Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts. USP/SP[Custom Size Christmas Gift Personalized Watch Boxes for Men]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[IMTEK High Precision and Rigidity Linear Guides for CNC machinery]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Modern 4 Person Workstation with Computer Desk Modular Office Furniture: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[18V Lithium Battery Powered Cordless Drill]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Most Fashionable and High Quality Comfortable Single Seater Office Chair with Solid Chromed Castors]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Monocular Hunting Wargame Mini Scope Digital Night Vision]

High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Women Fashion Winter V Neck Long Sleeve Clothes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Forged Steel Pressure Sealed Bonnet Globe Valve]

High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness]

High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line, Part I.3D CNC Router for Wood Cutting Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Power From Han′s GS 2000W Laser Cutter]

Stainless Steel Screens / Wedge Wire Filter Element, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Amazon Armor TPU Silicone Phone Case for Honor 10 Lite.EPS Foam Sheet Extrusion Machine for Fast Food Box Making, 175-184[30mm Si Laser Reflection Mirrors Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Titanium Dioxide Anatase for Paper Making]

Whitaker,J.R.(1963),Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe. Analytical Chemistry35(12), 1950-1953[PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aluminum Mould Shoe Mould Making CNC Milling Router MachineAuthor for correspondence