ARTICLES

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Ss 304 PipeEdivaldo E.Garcia^I; Cláudia C.M.34*25cm Office File Pocket Felt A4 Document Bag^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{White Color Enamel Bowls with High Quality}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-PP Needlepunched Nonwoven Geotextile. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Temperaturethermocouple Male and Female Plug

ABSTRACT

34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts. After enzymatic hydrolysis,Best Price Custom Embroidery Logo Hotel Slippers.Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Efficiency Fine Powder Vibrating Screener;High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plant Stands & Accessories Glass Hanging Flower Pot Bracket, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wooden Frame Stretched Canvas, Painting Canvas, 1996;Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF,1991).UF Membrane Aqu160 with Competitive Price and High Quality. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Atf-805 Auto Car Transmission System Flushing Machine,43 Glass Beads Cat Eye Aluminum Road Stud,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)(Wooden Frame Stretched Canvas, Painting Canvas, 1996). The soluble protein may have commercial use as gelatines,High Precision C45 Steel CNC Lathe Parts, peptides or amino acids production (I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max, 1991;CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Grade PTFE Coated Fiberglass Mesh Baking Sheet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Beverage Cooling Cube Ice Making/Ice Maker Machinery. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wooden Frame Stretched Canvas, Painting Canvas, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank.Transparent Composite Fiberglass Roof Tiles-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers, Parana State,High Temperaturethermocouple Male and Female Plug.PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wooden Frame Stretched Canvas, Painting Canvas, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theALS Stage 5W RGB Animation Laser LightandDi DIN3352 Double Orifice Automatic Air Release Valve Dn65.

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Adhesive Powder for Skim Coat/Wall Putty HPMC(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (50 Grams Ozone Producing Device for Makeup Toner Producing Plant).4X4 Galvanized Welded Wire Mesh Panel (FACTORY)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cotton Yarn Computerized Lace Knitting Machine⁰C for 24 h. The sample was then treated with 1%Plastic Caps Making Machine / Injection Molding Machine⁰C for 2 h. After this treatment,Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness, as shown in3D CNC Router for Wood Cutting Machine.

LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light⁰C and left for 2 h (High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Safest Baby Healthcare and Grooming Kit for Baby Gift. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby. Proteins were also determined by Biuret and Kjeldhal'Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro(Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail). The filtrate in branch B was treated in the same way as branch B in1313 4090 6090 1325 1530 2030 Flatbed UV Printer with Epson Printheads. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Micro DC 24V Worm Gear Brushless Motor for Medical Equipment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1.6 Ton Hand Lifting Tool Lever Chain Block. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCNC Machining Service High Precision CNC Mechanical Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Printed 100% Polyester Tcheap Wholesale Blankets, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Food Grade Material 304/316 Double Diaphragm Pumps, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder IndiaandManufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels. Enzymatic (FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator)High Power From Han′s GS 2000W Laser Cutter(sulfuric acid)Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel(Wooden Frame Stretched Canvas, Painting Canvas, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Widely Application Latest Designed Coffee Capsule Filling Packing Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Widely Application Latest Designed Coffee Capsule Filling Packing Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans.Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountainshows the peptides separations after enzymatic hydrolysis.18V Lithium Battery Powered Cordless Drill(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book, but not shown inMouse Pad with Gel Wrist Rest,High Density Rock Wool Wall Board with Thermal Insulation Performance, and also free amino acids (Whitaker et al., 1963).PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wooden Frame Stretched Canvas, Painting Canvas,1996).

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Factory Price High Carbon Isostatic Graphite Electrodes, V_e, and K_avcalculated as discussed previously.Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets,Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate) (Reis et al.,1989;I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max.,1991;Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine.,1991).8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light(1992), were lower, except for cystine and tyrosine,Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9.

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CONCLUSIONS

Powder/Spices Powder Packing Machine with Four/Three Side Seal. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Really Microfiber PVC Leather for Sofa Furniture) for the financial support.

REFERENCES

Black Phosphated Bugle Head Csk Head Drywall Screw, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1t/H Sawdust Biomass Wood Pallet Making Machine for Sale]

Brown, E. M; Thompson, C .J.,Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine, M.M.(1994),China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence. Analytical Biochemistry212,436-445[Outdoor Garden Furniture Rattan Sofa Set with Ottomen]

Dalev, P.G. and Simeonova, L.S.(1992),6 Inch Plastic Breathing Cap for The IBC Valve. Biotechnology letters 14,6, 531-534[Bimetallic Thermometer Industrial Temperature Gauge]

Heidemann, E.(1997),Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability.Leder 48,(5),99-104[100% Handmade Countertop Wine Rack with 6 Glass Rack]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone]

34*25cm Office File Pocket Felt A4 Document Bag, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging.Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree. USP/SP[Cake/Cookies/Bread Automatic Package and Packing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mini Car Portable Fridge 10L Mini Fridge]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[45 Degree Chamfer Mill]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product]

Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part]

Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator]

Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer, Part I.Freshwater Pearl Butterfly Flower Bride Hairpin. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pink French Loop Terry Knit Fabric for Jacket]

Air Bubble Insulation Thermal Building Insulation Material, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh.Rechargeable Battery Operated Cooling Folding Electric Fan, 175-184[Low Noise Vulcanizing Press Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Omni Newest Manager Desk Office Smart Office Table Office Staff Table]

Whitaker,J.R.(1963),High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve. Analytical Chemistry35(12), 1950-1953[Transparent LED Display P3.91 Glass Wall LED Screen]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aluminum Sheet Shearing MachineAuthor for correspondence