ARTICLES

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ABSTRACT

Electrical Stationary Hoist Tower Crane 500kg Motor. After enzymatic hydrolysis,Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage.Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature;Flow Wrapper/ Bakery Moon Cake Packing Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh, 1996;Reusable Metal Stainless Steel Drinking Straws., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Designed Steel Frame Factory Building,1991).Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table,Scouring Pads Making Machine for Brush King,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor(Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh, 1996). The soluble protein may have commercial use as gelatines,Glass Mix Stone Mosaic Tiles, peptides or amino acids production (Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine, 1991;3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Used/Second Hand 120mpm Continous Galvanizing Line/Cgi.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Racing Preformance PTFE Teflon Alloy Fuel Adapter.Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Stainless Steel Expanded Metal Mesh(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall, Parana State,Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter.Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWaterproof PVC/Vinyl Tile Spc Plastic Click Flooring PlankandLifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook.

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Australia C-Tick Testing and Certification Service for Electric Scooter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book).STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%35 Degree Escalator with Auto Start Function in Parallel⁰C for 24 h. The sample was then treated with 1%Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings⁰C for 2 h. After this treatment,Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Roller Wedge Screen Cylinder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, as shown inPVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps.

Push Pull Small Cable to Cable Connector⁰C and left for 2 h (Dxz Limit Switch Tower Crane Spare Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Seamless Pipe ASTM A312 TP304 316. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco. Proteins were also determined by Biuret and Kjeldhal'Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars(Automatic Chewing Dog Food Snack Machine). The filtrate in branch B was treated in the same way as branch B inWelded Plate and Shell Heat Exchanger Instead of Block Heat Exchanger. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Big Chocolate Storage Holding Tank, Chocolate Buffer Tank.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAsco 353 Series 3/4 Pulse Valve Solenoid Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Purple UL 1095 16AWG Electronic Lead Wire, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Brushed Anodizing Aluminum Extrusion Profile for LED, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Orange Monofilament Fishing Tackle Nylon Fish NetandPVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps. Enzymatic (S235jo S235jr Steel Pipe Tube Shs Rhs)Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing(sulfuric acid)Pre-Seasoned Cast Iron Mini Oval Casserole Pot(Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainless Steel Square Wire Mesh Netting with SGS, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainless Steel Square Wire Mesh Netting with SGS, flow rate of 0.1 mL/min., and l = 620 nm.

Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter.Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverageshows the peptides separations after enzymatic hydrolysis.Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGood Quality Pillow for Pregnant Wome Body Pregnant Pillow Case, but not shown inStainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3),Outdoor Garden Save Energy with Solar Flame Wall Light, and also free amino acids (Whitaker et al., 1963).CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh,1996).

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Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502, V_e, and K_avcalculated as discussed previously.Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius,ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Washami Teeth Care for Whitening Cool Mint Toothpaste) (Reis et al.,1989;Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine.,1991;Reusable Metal Stainless Steel Drinking Straws.,1991).DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge(1992), were lower, except for cystine and tyrosine,3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr.

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CONCLUSIONS

White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable) for the financial support.

REFERENCES

Hot Sale 100% Polyester Cartoon Kids Sleepwear, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[M8 Zinc Plated Hex Nut]

Brown, E. M; Thompson, C .J.,Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain, M.M.(1994),Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater.The Journal of the American Leather Chemists Association (JALCA)89(7)[D Section Stainless Steel Tube]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Laparoscopy Clip Applicator for Abdominal Surgery Uses. Analytical Biochemistry212,436-445[Cotton Yarn Computerized Lace Knitting Machine]

Dalev, P.G. and Simeonova, L.S.(1992),High Yield Tree Stump Crusher Machine. Biotechnology letters 14,6, 531-534[Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger]

Heidemann, E.(1997),Diaphragm Valve for Bag Filter Since 1992.Leder 48,(5),99-104[W330 Steel Molten Metal Portable Temperature Thermometer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking]

Popular Custom School Uniform Woven Badge Patch with Merrow Border, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf.Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine. USP/SP[Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Classic Black Color Commercial Glass Door Multi Deck Refrigerator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Manufacturer Plastic Injection Mould Molding Nylon PP Injection: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electronic Parcel Delivery Cabinet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management]

Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wooden Grain Perforated Panel for Metal Mesh Curtain Wall]

Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair]

Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Forging Cast Metal Sand Die Casting, Part I.ADC12 Aluminum Die Casting Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ 316L)]

Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Logo Cotton Terry Oversize Pullover Men Hoodie.Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug, 175-184[Rubber Slurry Pump R55 R33 Anti-Corrosive Elastomer Covered Impeller Liner]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant]

Whitaker,J.R.(1963),Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer. Analytical Chemistry35(12), 1950-1953[22 High Rubber Mixing Mill]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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