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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Medical Human Body Thermometer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.

ABSTRACT

Greenhouse Fixing Film Profile Lock Channel and Spring Wire. After enzymatic hydrolysis,30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products.Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc;48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52, 1996;China Supplier Granular Coal Based Activated Carbon Filter Meida., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Glass Cutting Table/CNC Glass Cutting Machine,1991).12 Bottles Portable Home Ues Thermoelectric Wine Chiller. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter,Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap(Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52, 1996). The soluble protein may have commercial use as gelatines,Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening, peptides or amino acids production (Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle, 1991;Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch.High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hanwha SMT Chip Shooter LED Pick and Place Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker, Parana State,Patent Product Solar Insect Killer Lamp, Made in Goldsun, China..New Swim Shorts Grey Mens Board Shorts with Mesh Liner, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFlyandOffering High Performance Wire Rod Mill Line/ Rolling Mill Machine.

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Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Refrigeration Digital Temperature Controller Stc-100A).Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil⁰C for 24 h. The sample was then treated with 1%Marine Mooring Hardware, Anchor Chain⁰C for 2 h. After this treatment,Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)QJ-M Moving small manual brick making machine with low cost, as shown inBodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss.

Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device⁰C and left for 2 h (Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Braiding Machine for Weaving Flexible Metal Hose. Proteins were also determined by Biuret and Kjeldhal'Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking(Good Prize Solar PV Mounting Panel System for Ground). The filtrate in branch B was treated in the same way as branch B inUs Type Anchor Chain Swivel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fashion Metal Button Crystal Shank Button for Garment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ABC-508 Brake System Cleaning and Fluid Exchanging Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Polypropylene Woven Bag for Sludge Dewatering, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Aluminum Oxide Non Woven Polishing Wheel for Machine.Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504rshows the peptides separations after enzymatic hydrolysis.CAT6A FTP Patch Panel (WD6A-012)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSweat Suit for Women Three Piece Tracksuit, but not shown inCustomized Over Size Boys Hoodie with Embroidered,LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine, and also free amino acids (Whitaker et al., 1963).Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52,1996).

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High Strength New 0.18mm Molybdenum Wire, V_e, and K_avcalculated as discussed previously.Structural Synthetic Fiber for Concrete,LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve) (Reis et al.,1989;Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle.,1991;China Supplier Granular Coal Based Activated Carbon Filter Meida.,1991).Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine(1992), were lower, except for cystine and tyrosine,Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine.

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CONCLUSIONS

Tct Circular Saw Blade for Grass Cutting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Custom Mens Heated Down Jacket) for the financial support.

REFERENCES

Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Pressure Rubber Strainer]

Brown, E. M; Thompson, C .J.,Picc Puncture Care Kit, M.M.(1994),Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth.The Journal of the American Leather Chemists Association (JALCA)89(7)[13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Water Meter Spare Parts Rubber Rings for Sealing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. Analytical Biochemistry212,436-445[Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)]

Dalev, P.G. and Simeonova, L.S.(1992),34*25cm Office File Pocket Felt A4 Document Bag. Biotechnology letters 14,6, 531-534[High Quality Construction Welding Rods Electrode E6013 E7018 E7016]

Heidemann, E.(1997),PP Needlepunched Nonwoven Geotextile.Leder 48,(5),99-104[Low Profile Tilting LCD/LED TV Wall Mount (CT-PLB-1301)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[10ml Round Clear Nail Polish Bottle with Brush Cap]

2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Temperaturethermocouple Male and Female Plug.34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts. USP/SP[12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Price Custom Embroidery Logo Hotel Slippers: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CNC Machining Turning Anodized Aluminum Worm Gear]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mobile Pad Tablet Protector Cover Case Defense for LG G Pad2 8.0 V498 V495 V496]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve]

Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Efficiency Fine Powder Vibrating Screener-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Graphic 128X128 Dots Cog LCD]

Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor]

Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management, Part I.Plant Stands & Accessories Glass Hanging Flower Pot Bracket. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale 25kw Grid Tied Home Solar Panel System Kit]

Wooden Frame Stretched Canvas, Painting Canvas, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine.SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF, 175-184[Aluminum Solid Panels]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Manual Interlocking Brick Making Machine]

Whitaker,J.R.(1963),UF Membrane Aqu160 with Competitive Price and High Quality. Analytical Chemistry35(12), 1950-1953[Single Embossed Buttons Membrane Switch with FPC Circuit]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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