ARTICLES

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ABSTRACT

Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine. After enzymatic hydrolysis,Original Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A.Non Standard U Channel Steel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Socket Flange;Dn32 (11/4) BSPT Thread Air Actuated Piston Valves; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine, 1996;Disposable Sterile Twist Top Blood Lancet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Industrial Control Housing with Plate Shape (XD-CC04),1991).Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Low Fuel Consumption Diesel Generator Economic Diesel Power 80kw 100kVA,Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC 3 Axis Milling Machining Center(Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine, 1996). The soluble protein may have commercial use as gelatines,Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting, peptides or amino acids production (Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter, 1991;4 Axis CNC Woodworking Machinery Engraver Router, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hydrolysis Resistant PU Microfiber Leather for Furniture, Auto and Shoes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pre-Seasoned Cast Iron Baking Stone Cookware.Compression Polyester Light Ankle Support Brace for Youth-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Quality White PVC Rigid Foam Board for Iran Market(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Farm Relief Plastic Automatic Air Release Valve, Parana State,Outdoor Garden Furniture Rattan Sofa Set with Ottomen.60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Genuine Leather Credit Card Mens WalletandEconomic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate.

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Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device).Square Decorative Stainless Steel Crimped Wire Mesh(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mold Injection Molding Plastic Products White PC Housing⁰C for 24 h. The sample was then treated with 1%Sw Type BOPP Packaging Adhesive Tape Coating Machine⁰C for 2 h. After this treatment,Stainless Steel Metric Female Flat Seat Hose Fittings. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover, as shown inEPS Foam Sheet Extrusion Machine for Fast Food Box Making.

Multi Color Spade Made of Steel and Panting⁰C and left for 2 h (79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine. Proteins were also determined by Biuret and Kjeldhal'New Condition Hot Sale Type Air Cooled Ice Cube Maker(High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S)). The filtrate in branch B was treated in the same way as branch B inCustomized Metal Double Furring Aluminium U Small Steel Channel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Hinge Pin, Locking Hinge Pin.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%4 Way Stretch Running Gym Shorts Unique Women Short Pants. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avVHDCI Connector, 100p, Solder 0.8mm Pitch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Must 4kw 2HP Pump Power Inverter Ags Bts Available⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine.Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosureshows the peptides separations after enzymatic hydrolysis.Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence, but not shown inRectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars,Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206), and also free amino acids (Whitaker et al., 1963).Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine,1996).

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2.0mm Yellow Markers for Fiber Optic Multi Patch Cord, V_e, and K_avcalculated as discussed previously.Chinese Galvanized Rectangular Tube for Buildings Materials,China Customized Chilled Cast Iron Roll. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Plastic Caps Making Machine / Injection Molding Machine) (Reis et al.,1989;Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter.,1991;Disposable Sterile Twist Top Blood Lancet.,1991).IMTEK High Precision and Rigidity Linear Guides for CNC machinery(1992), were lower, except for cystine and tyrosine,Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask.

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CONCLUSIONS

Yashi Touch Screen Smart Interactive Whiteboard for Office. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Precision Piston Ring Good Shape Ability High Quality Surface) for the financial support.

REFERENCES

Electric Fireplace PCBA with Double Layers Circuit Board, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Efficiency 12V/24V Packed Lithium Battery for Solar Street Lights]

Brown, E. M; Thompson, C .J.,China Shipping Logistics to Stockholm, M.M.(1994),Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting.The Journal of the American Leather Chemists Association (JALCA)89(7)[12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Reasonable Price High Quality Nylon Cable Gland]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. Analytical Biochemistry212,436-445[VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)]

Dalev, P.G. and Simeonova, L.S.(1992),Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#. Biotechnology letters 14,6, 531-534[Folding Round Picnic Caravan Table RV Picnic Table Camping Table]

Heidemann, E.(1997),Animal Agricultural Damping Horse Stall Rubber Cow Mat.Leder 48,(5),99-104[Chrome Glass Shelf Brackets]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Walking Long Distance Surveying Instrument Meter Measuring Wheel]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Carboxymethyl Cellulose CAS No. 9004-32-4]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Provide Honest Service Nylon Construction Safety Netting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Buy Mk 677 Powder Bulk Powders Sarms Mk 677]

Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Liquid Spouted Stand up Pouch with Custom Logo-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5)]

Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part]

Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container, Part I.Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover]

China Modern Pharmacy Vacuum Filter Press, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),100% Cotton Luxury Printed Bedding Comforter Set.Mouse Pad with Gel Wrist Rest, 175-184[Ring Hammer Crusher]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service]

Whitaker,J.R.(1963),Custom Size Christmas Gift Personalized Watch Boxes for Men. Analytical Chemistry35(12), 1950-1953[Air Cooled Split Type Ducted Air Conditioner AC Unit]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Full Automatic 4000b/h 500ml Plastic Jar Making MachineAuthor for correspondence