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ABSTRACT

Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker. After enzymatic hydrolysis,Corrugated Board Production Line.Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hex Thin Head Nut, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2, 1996;Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Spare Parts for Caterpillar Construction Machinery,1991).Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,50 Grams Ozone Producing Device for Makeup Toner Producing Plant,Flat Pack Temperary Container House 20FT 40FT,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sweat Suit for Women Three Piece Tracksuit(Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2, 1996). The soluble protein may have commercial use as gelatines,Cold Rolled Stainless Steel Strip /Coils 410/430/409, peptides or amino acids production (FRP Water Tank GRP Fishing Tanks From Factory Supply, 1991;Decorative House Stone Coated Metal Roof Tile, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine.PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Blue Steel Packing Strip(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio).Home Products 16′ Perforated Flex Pipe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1220 Fork Length 3-Wheel Electric Forklift Truck⁰C for 24 h. The sample was then treated with 1%Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits⁰C for 2 h. After this treatment,FM USB Radio Audio Power Amplifier for Home Bar Club. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sell Mechanical Bar Screen for Water Treatment, as shown in34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts.

Glow Powder for Luminous Coating⁰C and left for 2 h (CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.700 mm RP HP UHP Graphite Electrode Sold at Factory Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck. Proteins were also determined by Biuret and Kjeldhal'Union Metal Steel Building and Construction Material(Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay). The filtrate in branch B was treated in the same way as branch B inSmall Volume Super Thin Hose Crimper (JKL500). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%450ml Sublimation Stainless Steel Milk Bottle (Colorful). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLower Noise Colled Rolled Ball Screw(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3.0kw Spindle CNC Router with Certificate for Woodworking⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/800W Electric Motor Price Lawn Mower Motor Concrete Mixer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Air Cooled Split Type Ducted Air Conditioner AC Unit, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Density Celuca Kitchen Cabinets PVC Foam Board. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality) for the financial support.

REFERENCES

2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Permanent Formwork For Concrete]

Brown, E. M; Thompson, C .J.,E171 Standrad Titanium Dioxide Food Grade A200, M.M.(1994),Elbow Forming Machine Gorelocker HVAC Duct Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Professional Factory Supply Design Cat Tree Pet Product]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Barbecue Net BBQ Grill Grates Wire Mesh. Analytical Biochemistry212,436-445[PRO Audio Loudspeaker PA Speaker System Line Array Speaker]

Dalev, P.G. and Simeonova, L.S.(1992),Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses. Biotechnology letters 14,6, 531-534[Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder]

Heidemann, E.(1997),L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture.Leder 48,(5),99-104[For Iphone 5 Oca Glass Lcd]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Made Colorful Sublimation Womens Yoga Pants Wholesale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Groove Type Wood Log Tree Peeling and Debarking Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Change/Upgrade 620W/650W/1000W YAG Laser Cutting Machine to Fiber Laser Cutting Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hex Thin Head Nut]

Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture]

Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails]

Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Fashion PVC Sequins Adjustable Women Brace Suspenders, Part I.Solid Flex-Drain Pipe Length 40′. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet]

Whitaker,J.R.(1963),W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/. Analytical Chemistry35(12), 1950-1953[Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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