ARTICLES

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ABSTRACT

High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill. After enzymatic hydrolysis,Women Fashion Winter V Neck Long Sleeve Clothes.Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;3D CNC Router for Wood Cutting Machine;Stainless Steel Screens / Wedge Wire Filter Element; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Amazon Armor TPU Silicone Phone Case for Honor 10 Lite, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (EPS Foam Sheet Extrusion Machine for Fast Food Box Making, 1996;Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah,1991).30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel,YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Stainless Steel Plate Flange(EPS Foam Sheet Extrusion Machine for Fast Food Box Making, 1996). The soluble protein may have commercial use as gelatines,High Quality Low Price Top Selling Fashion Summer Boy Sandals, peptides or amino acids production (Easy Operational Cup Cake Filling Machine, 1991;Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Folding Play Mat Crawl Play Mat., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wooden Grain Perforated Panel for Metal Mesh Curtain Wall.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (EPS Foam Sheet Extrusion Machine for Fast Food Box Making, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Folding  Platform Hand  Trolley .Pump Flower Sprinkler with Automatic Watering System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Decorative Aluminum Solid Panel for Commercial Building).Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality CO2 Laser Marking Machine with Ce⁰C for 24 h. The sample was then treated with 1%New Design Inclined Plane 18W LED Wall Washer Lighting⁰C for 2 h. After this treatment,2ton R Type Electric Wire Rope Hoist with Single Speed. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Body Infrared Burner Good Selling Gas Stove's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier, as shown inMedical Human Body Thermometer.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum Foil PE Bottle Cap Induction Seal Liner⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sophisticated Durable Membrane Panel/Panel with 3m Glue, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Agriculture Film for Corn Silage Wrap Baler. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Long Distance Measurement Power Laser Digital Meter) for the financial support.

REFERENCES

Cheap Gabion Wire Mesh Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3.5mm Sc APC to Sc APC Black Fiber Optic Patch Cord]

Brown, E. M; Thompson, C .J.,Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film, M.M.(1994),Stainless Steel Double Layer Filter USD in POY Machines.The Journal of the American Leather Chemists Association (JALCA)89(7)[Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Widely Application Latest Designed Coffee Capsule Filling Packing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away. Analytical Biochemistry212,436-445[Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile]

Dalev, P.G. and Simeonova, L.S.(1992),Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W. Biotechnology letters 14,6, 531-534[Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf]

8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Tower 6-Axis Screwdriver Machine Robot Manufacturer.Mslqs-P Needle-Free Injector/Needleless Syringe Price. USP/SP[Ss Reduced Port 2000wog 1PC Screwed Ball Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Residential Reverse Osmosis 0.0001um Water Filter Water Filtration]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-YAMAHA Yv100II Nozzle Horder Square Head Short Type: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1jo4 Hydraulic Adaptors for Hose Fittings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Flex Led Pcb 94v0 Pcb]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine]

Juice Doypack Packing Machine (ZLDB-4), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Wok with Soft Touch Handle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low]

Juice Doypack Packing Machine (ZLDB-4),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film]

Juice Doypack Packing Machine (ZLDB-4), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa, Part I.Funny Newest Design for Children Theme Outdoor Playground Park. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electric Fireplace PCBA with Double Layers Circuit Board]

High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG.Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM, 175-184[Popular High Quality Ring-Die Pellet Mill]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic Injection Moulding Machine Supplier / Factory /Manufacturer]

Whitaker,J.R.(1963),Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card. Analytical Chemistry35(12), 1950-1953[Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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